Nowadays, three methods which target the evaluation of the characteristics of ecotoxicology tests according to aquatic trophic level are well-known, approved methods. In Turkey, the toxicity dilution factor (TDF) method, approved by Turkish Water Pollution and Control Regulation, and Lebistes reticulatus the actual method name that utilizes this animal which is a high level consumer, are used in the assessment of acute toxicity levels of waste waters with different compositions. However; it is not preferred by researchers except statutory obligations due to its disadvantages in implementation. The other method Daphnia magna, which belongs to secondary trophic group, is also widely used. Vibrio fischeri which is based on bioluminescent bacteria is mostly preferred due to ability of giving fast and precise results in acute toxicity assessment tests. In this study, specific to Turkey, it is aimed to compare issues such as experimental process, time, cost, efficiency, repeatability, etc. of tests that take place in the related legislation and standards. In light of all this information, it is determined the insufficient parts about legal regulations related to toxicity in Turkey. It can be suggested that bioluminescence bacteria (V. fischeri), defined all around the world recently with its precision, optimal cost, repeatability features be used in Legal Regulations of Turkey. It is aimed to contribute to scientific literature, this study being the first on suggesting an innovative approach about acute toxicity test in Turkey.