Evaluation of delphinidin as a storage medium for avulsed teeth

Nam, Ok Hyung; Ro, Sang Tae; Lee, Hyeon‐Woo; Jeong, Jaeki; Chae, Yong Kwon; Lee, Ko Eun; Choi, Sung Chul; Kang, Sang Wook

doi:10.1186/s12903-023-02713-9

Cited by 6 publications

(6 citation statements)

References 40 publications

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“…In cases where prolonged extra oral time is unavoidable, the tooth should be maintained in a suitable media, such as milk, Viaspan, Hanks balanced salt solution (HBSS), saline or individual saliva, contact lens solution, Save-a-tooth (Phoenix-Lazerus Inc., Shartlesville PA, USA), and EMT Tooth saver (SmartPractice.com, Phoenix, AZ, USA) until it is replanted by a pediatric dentist or General dentist. [28][29][30] The preferred storage medium recommended by The American Association of Endodontists is Hanks balanced salt solution (HBSS), because of its ability to preserve the vitality of periodontal ligament (PDL) cells for a longer duration. 31 HBSS preserves the vitality of fibroblast cells for 72 h, but is not commonly available in MI rooms.…”

Section: Discussionmentioning

confidence: 99%

Tooth avulsion and its consequences: Case series

Kapri,

Rana,

Jacob

et al. 2023

APRD

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On an average about 30% of all school children suffer traumatic dental injury in primary dentition, whereas 22% suffer in permanent dentition. Avulsion is a serious injury which causes damage to dental and supportive tissues, ranging from 0.5-16 % among dental injuries and it mostly occurs in maxillary incisors. Tooth avulsion can be described as the complete displacement of the entire tooth out of its alveolar socket as a result of force generated due to external injury. The accepted management of choice is immediate replantation of the tooth into its socket provided dental facilities are nearby available and if it is delayed, placement of the tooth in an appropriate storage media until dental treatment can be initiated. Replantation of avulsed incisors in children has shown successful prognosis. The replanted teeth with time may lead to ankylosis followed by inflammatory or replacement resorption, as immediate replantation is practically rare. This case series showcases management of an avulsed maxillary left central incisor which was kept in patient oral cavity from the moment of trauma until its replantation in a eight year old boy. The avulsed tooth was replanted back into the alveolar socket after completion of endodontic treatment extra orally in same visit and splinted with composite resin. The other 2 case reports are 01 & 1.5 years follow up of replantated avulsed central incisors. During the follow up of these cases it was found that the teeth reported in these cases have remained in a stable, functional position but revealed external root resorption & replacement resorption even after completion of endodontic treatment. The amount of damage to tooth and supporting structures like bone, gingiva, emergency management and follow up period play a vital role in the prognosis of the avulsed tooth. It can be recommended to keep the avulsed tooth in saline solution as it is easily available when more appropriate storage media like HBSS are not available immediately.

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Section: Discussionmentioning

confidence: 99%

Tooth avulsion and its consequences: Case series

Kapri,

Rana,

Jacob

et al. 2023

APRD

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“…For evaluating anti-inflammatory effects of AG and AGH, a NO assay was performed following the standard method with some modifications [23]. The cells (n = 4) were seeded To characterize the particles, AG and AG via hot-melt extrusion (AGH) samples were coated with platinum for 60 s using a sample-coating machine (EM ACE600, Leica, Germany) and photographed under a scanning electron microscope (SEM; S-4800, Hitachi, Japan).…”

Section: Nitric Oxide (No) Assaymentioning

confidence: 99%

“…For evaluating anti-inflammatory effects of AG and AGH, a NO assay was performed following the standard method with some modifications [23]. The cells (n = 4) were seeded…”

Section: Nitric Oxide (No) Assaymentioning

confidence: 99%

“…For evaluating anti-inflammatory effects of AG and AGH, a NO assay was performed following the standard method with some modifications [23]. The cells (n = 4) were seeded into 6-well plates with a density of 2 × 10 5 cells/well, incubated for 24 h, and treated with 10 µg/mL of LPS from Porphyromonas gingivalis (InvivoGen, San Diego, CA, USA) for 24 h. AGH solutions (125 µg/mL) were added into the wells 1 h before LPS treatment.…”

Section: Nitric Oxide (No) Assaymentioning

confidence: 99%

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Accelerated Oral Healing by Angelica gigas Nakai from Hot Melt Extrusion Technology: An In Vitro Study

Ye,

Lee,

Park

et al. 2023

Medicina

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Background and Objectives: In spite of the oral environment being healing-prone, its dynamic changes may affect wound healing. The purpose of this study was to assess the oral wound healing effect of Angelica gigas Nakai (AG) prepared by hot-melt extrusion. Materials and Methods: Human gingival fibroblast (HGF) cells were treated with AG or AG via hot-melt extrusion (AGH) for 24 h to determine the optimal concentration. For evaluating the anti-inflammatory effect of AG and AGH, a nitric oxide assay was performed under lipopolysaccharide (LPS) stimulation. The wound-healing effects of AG and AGH were evaluated using cell proliferation/migration assays and wound-healing marker expression through qRT-PCR. Results: Both AG and AGH showed no cytotoxicity on HGH cells. Regarding nitric oxide production, AGH significantly decreased LPS-induced nitric oxide production (p < 0.05). AGH showed a significantly positive result in the cell proliferation/cell migration assay compared with that in AG and the control. Regarding wound healing marker expression, AGH showed significantly greater VEGF and COL1α1 expression levels than those in the others (p < 0.05), whereas α-SMA expression was significantly different among the groups. Conclusions: Within the limits of this study, AGH accelerated oral wound healing in vitro.

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“…Another study evaluated the suitability of 10% propolis solution as a transport medium for avulsed teeth [12]. Delphinidin, a type of anthocyanidin found in blueberries, has also been reported to preserve the viability of PDL cells and exhibit anti-inflammatory effects [13].…”

Section: Introductionmentioning

confidence: 99%

Effect of Polydeoxyribonucleotide on Human Periodontal Ligament Cells as a Storage Medium for Avulsed Tooth

Ro,

Chae,

Lee

et al. 2023

J Korean Acad Pediatr Dent

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Objective: This study aimed to evaluate the suitability of polydeoxyribonucleotides (PDRN) as a storage medium for avulsed teeth.Materials and Methods: The viability of human periodontal ligament (PDL) cells stored in Hank’s balanced salt solution and PDRN solutions (concentrations, 10, 25, 50, and 100 μg/mL) and tap water was measured using the Cell Counting Kit-8 and Live/Dead assays. In addition, Nitric oxide detection and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to evaluate the anti-inflammatory effect of PDRN.Results: The viability of PDL cells stored in a 100 μg/mL PDRN solution was significantly higher than that of cells stored in the other solutions (p < 0.01). Furthermore, cells stored in 100 μg/mL PDRN solution demonstrated a significantly reduced NO production (p < 0.0001), and cells stored in 50 and 100 μg/mL PDRN solutions expressed significantly lower levels of tumor necrosis factor α, interleukin (IL) -4, IL-6, and IL-10 (p < 0.01) compared to cells stored in HBSS.Conclusion: The PDRN solution exhibited cell-preserving and anti-inflammatory effects on the PDL cells. The findings of this study can serve as a basis for further experiments directed at the development of an effective storage medium for avulsed teeth.

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Evaluation of delphinidin as a storage medium for avulsed teeth

Cited by 6 publications

References 40 publications

Tooth avulsion and its consequences: Case series

Tooth avulsion and its consequences: Case series

Accelerated Oral Healing by Angelica gigas Nakai from Hot Melt Extrusion Technology: An In Vitro Study

Effect of Polydeoxyribonucleotide on Human Periodontal Ligament Cells as a Storage Medium for Avulsed Tooth

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