Evaluation of di‐potassium and tri‐potassium EDTA evacuated tubes for routine haematological testing

Abstract

Background The influence of blood collection devices on laboratory tests is often overlooked. We have discussed here how blood collection materials and devices can alter haematological test results, with an emphasis on blood collection tube (BCT) additives. We have compared K2 & K3‐EDTA vacuum tubes of same manufacturer IMPROVACUTER ® for CBC analysis. Methods Blood was collected in both tubes simultaneously. Samples were analysed immediately after collection (<15 minutes) and after 4 hours by MEK−6318J/K Haematology Analyzer. Paired student's t‐test was applied for statistical analysis. Significant differences among results and biases were compared with current quality specifications. Results Significant differences were found in Neutrophyls (GR) and mean platelets volume (MPV) when compared. K2‐EDTA vs K3‐EDTA(1st time) after< 15 minutes of sample collection, for Monocytes counts (MO), MCV, MPV, and RDW when comparing K2‐EDTA (first time) vs K2‐EDTA(second time, after 4 hours); and for Monocytes counts (MO), MCV, MPV, PCT, and RDW when comparing K3‐EDTA(1st time) vs K3‐EDTA(2nd time) in order to check the stability and reproducibility of vacutainers. Conclusions This study assesses the use of K2& K3‐EDTA vacuum tubes whether or not they represent a bias in haematological testing (CBC). The use of K2‐EDTA and K3‐EDTA vacuum tubes represent a clinically relevant source of variation for MCV, MPV, RDW, and PCT.

Evaluation and Comparison of Stability and Reliability of CBC Parameters Determined by Using Automatic Celltac G MEK-9100 Hematology Analyzer during Extended Storage at 4°C

“…However, error aspect for studies in the pre analytical phase requires considerable attention on procedures like blood sample collection, identification of patients, selection of proper blood collection vials, accurate blood suction, specimen labeling, clerical error and storage. Work by many others shows that 93% of errors does not relate to the high standard analytical method [2][3][4]. Central laboratories have been established in many countries in addition to local initiative hospital laboratories.…”

Correction of spurious low platelet counts by optical fluorescence platelet counting of BC-6800 hematology analyzer in EDTA-dependent pseudo thrombocytopenia patients

“…EDTA is a widely used anticoagulant for whole blood routine hematologic examination ( 10 ), proposed by the International Council for Standardization in Hematology (ICSH). Since Shreiner and Bell first defined a phenomenon of platelet aggregation directly attributed to EDTA anticoagulant ( 1 ), numerous cases of EDTA-PTCP have been reported in patients with immune thrombocytopenic purpura ( 11 ), dengue infection ( 12 ), scrub typhus infection ( 13 ), sepsis ( 14 ), gastrectomy ( 15 ), neuroendocrine carcinoma ( 16 ), etc.…”

The Effect of LRP-1 Gene and Mir-205 on Cardiovascular Disease in Iraqi Patients with Familial Hypercholesterolemia

“…From each participant,2 ml of whole blood was needed to be collected from the venous blood directly into an EDTA containing tube, this procedure was done under sterile conditions [19].…”