Evaluation of Diagnostic Performance of RAPIDEC CARBA NP Test for Carbapenemase-Producing<i>Enterobacteriaceae</i>

Song, Wonkeun; Yoo, Gilsung; Hwang, Gyu Yul; Uh, Young

doi:10.5145/acm.2016.19.3.59

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…The RAPIDEC CARBA NP test demonstrated an overall sensitivity of 69.3% in comparison to gold standard detection of meropenem and imipenem resistance by VITEK 2 system and E- test ( Table 2 and Table 3 ). Our results were less than that reported by other studies 22 , 29 , 30 which estimated the sensitivity of the RAPIDEC CARBA NP test for detecting CRE to be 89.8%, 93.9%, and 98.8%, respectively. The type of beta-lactamase enzymes carried by CRE isolates contributes to the variations in sensitivity and specificity of this test.…”

Section: Discussioncontrasting

confidence: 97%

Detection of Carbapenem-resistance in CRE by Comparative Assessment of RAPIDEC® CARBA NP and Xpert™Carba-R Assay

El-Tahlawi¹,

Jiman-Fatani²,

Gad³

et al. 2023

IDR

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Introduction Carbapenem-resistant Enterobacteriaceae (CRE) infections resist nearly most available antimicrobials, resulting in poor clinical outcomes. Saudi Arabia has a relatively high CRE prevalence. This study aims to evaluate the sensitivity of Rapidec Carba NP test and GeneXpert Carba-R assay compared with conventional manners for detection of carbapenemase-producing Enterobacteriaceae . Methods This is a cross-sectional study including a total of 90 CRE isolates examined at two tertiary hospitals in KSA from October 2020 to December 2021. Gram-negative Enterobacteriaceae were identified by using Vitek 2 system and were furtherly tested for imipenem and meropenem susceptibility by E- test strips, followed by Rapidec Carba NP test and the Xpert™Carba-R assay. Results Carbapenem-resistant K. pneumoniae (78.9%) and carbapenem-resistant E. coli (14.4%) were the two most common isolates species. Colistin (98.9%) and tigecycline (88.9%) were the most effective antibiotics against CRE isolates, followed by amikacin (52.2%), gentamicin (33.3%), cotrimoxazole (15.6%), and ciprofloxacin (8.9%). blaOXA-48 was the predominant carbapenemase gene (44.4%), followed by blaNDM (32.2%). blaKPC gene was not detected. The Rapidec Carba NP and the Xpert™Carba-R demonstrated an overall sensitivity of 69.3% and 88%, respectively, in comparison to gold standard detection of meropenem and imipenem resistance by Vitek 2 system and E- test strips. Discussion RAPIDEC ® CARBA NP may be a beneficial screening test for detecting CRE, but for confirmation of the results, Xpert Carba-R assay is more sensitive, significantly lowering the turnaround time compared to reference traditional methods. The information on carbapenemase genes may be used for epidemiologic purposes and outbreak management.

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Section: Discussioncontrasting

confidence: 97%

Detection of Carbapenem-resistance in CRE by Comparative Assessment of RAPIDEC® CARBA NP and Xpert™Carba-R Assay

El-Tahlawi¹,

Jiman-Fatani²,

Gad³

et al. 2023

IDR

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“…The probabilities of the presence of E. coli by VITEK 2 and MicroScan were 99% and 99.9%, respectively. To confirm species identification and antimicrobial susceptibilities, a modified Hodge test, carbapenemase inhibition test, XpertCarba-R assay (Cepheid, Sunnyvale, CA, USA), and REBA-EAC assay (a PCR-based reverse blot hybridization assay for detection of extended-spectrum β-lactamases, AmpC β-lactamases, and carbapenemases) were performed ( Table 1 ) [ 8 9 ]. PCR-amplified products of the bla NDM and bla OXA-48-like genes were sequenced using in-house primers NDM-290F (5′-GTT GGT CGA TAC CGC CTG GAC CGA T-3′) and NDM-516R (5′-AGTCAGGCTGTGTTGCGCCGCAAC-3′), OXA48-80F (5′-GTT GGA ATG CTC ACT TTA CTG-3′) and OXA48-279R (5′-AAG ACT TGG TGT TCA TCC TTA AC-3′), respectively.…”

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confidence: 99%

NDM-5 and OXA-48 Co-producing Uropathogenic Escherichia coli Isolate: First Case in Korea

Jhang

Wang²,

Yoo

et al. 2018

Ann Lab Med

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“…All stool swab specimens for CPE screening were inoculated in the selective chromogenic medium (CHROMagar KPC, Hangang, Gunpo, Korea). The CRE isolates obtained from the clinical specimen were tested by the Modified Hodge test, carbapenemase inhibition test, and CarbaNP test (bioMérieux, Durham, NC, USA) [6], and then, the CPE suspects were tested by XpertCarba-R assay (Cepheid, Sunnyvale, CA, USA) [5]. Each time a CPE was isolated, the subcultured colony was delivered to the Korea Centers for Disease and Prevention (KCDC) for the confirmation of the carbapenemase genotype, and KCDC gave feedback about the type of carbapenemase genes present.…”

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confidence: 99%

Nosocomial Outbreak Caused by NDM-5 and OXA-181 Carbapenemase Co-producing Escherichia coli

et al. 2019

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Carbapenemase-producing Enterobacteriaceae (CPE) is an important and increasing threat to global health. From July to September 2017, 20 inpatients at a tertiary care hospital in Korea were either colonized or infected with carbapenem-resistant Escherichia coli strains. All of E. coli isolates co-produced bla NDM-5 and bla OXA-181 carbapenemase genes and shared ≥88% clonal relatedness on the basis of a cladistic calculation of the distribution of pulsed-field gel electrophoresis patterns. Rapid detection of CPE is one of the most important factors to prevent CPE dissemination because it takes long time for CPE to become negative.

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Evaluation of Diagnostic Performance of RAPIDEC CARBA NP Test for Carbapenemase-ProducingEnterobacteriaceae

Cited by 6 publications

References 13 publications

Detection of Carbapenem-resistance in CRE by Comparative Assessment of RAPIDEC® CARBA NP and Xpert™Carba-R Assay

Detection of Carbapenem-resistance in CRE by Comparative Assessment of RAPIDEC® CARBA NP and Xpert™Carba-R Assay

NDM-5 and OXA-48 Co-producing Uropathogenic Escherichia coli Isolate: First Case in Korea

Nosocomial Outbreak Caused by NDM-5 and OXA-181 Carbapenemase Co-producing Escherichia coli

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