Evaluation of different algal species sensitivity to mercury and metolachlor by PAM-fluorometry

Juneau, Philippe; Dewez, David; Matsui, Saburo; Kim, Sang-Gil; Popovic, Radovan

doi:10.1016/s0045-6535(01)00034-0

Cited by 106 publications

(75 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The toxicity, measured as the decrease of in the yield of Photosystem II, Y (II), indicates that levulinates affect the electron flow. This effect is commonly induced by a wide range of chemicals such as Zn (Corcoll 2011, Corcoll et al 2011, Hg (Juneau et al 2001) and Cu (Juneau et al 2002), volatile compounds , herbicides Frank 1998, Conrad et al 1993, Dorigo and Leboulanger 2001) and ultraviolet radiation (UVR) (Navarro et al 2008). Even if the underlying processes explaining the levulinate toxicity are unknown, the behavior of minimal fluorescence (F) after 2 hours of exposure (data not shown) seems to point to 12 an attenuation of photosynthetic pigments, and particularly a decrease in chlorophyll a.…”

Section: Levulinate Toxicity In Algaementioning

confidence: 99%

Ecotoxicity studies of the levulinate ester series

et al. 2014

View full text Add to dashboard Cite

Abstract:The increasing interest in the development of novel green solvents has led to the synthesis of benign alternative products with minimized environmental impacts.However, most of published studies on green solvents focus primarily on their physicochemical properties, with limited emphasis on absence of ecotoxicological assessment. In this study, we evaluated the acute ecotoxicity of four levulinates (levulinic acid, methyl levulinate, ethyl levulinate and butyl levulinate) on freshwater algae (Chlamydomonas reinhardtii), bacteria (Vibrio fischeri), daphnids (Daphnia magna) and earthworms (Eisenia foetida) using various dose-response tests. As a general trend, the toxicity of levulinate esters in aquatic exposure (assessed as the EC 50 ) increased as a function of increasing alkyl chain length; accordingly, the most toxic compound for the aquatic organisms was butyl levulinate, followed by ethyl levulinate and methyl levulinate. The most toxic compound for Eisenia foetida (terrestrial exposure) was methyl levulinate, followed by ethyl levulinate, butyl levulinate and levulinic acid; in this case, we observed an inverse relationship between toxicity and alkyl chain length. Based on both the lowest EC 50 found in the aquatic media and the ratio between predicted environmental concentration (PEC) and the predicted no-effect concentration (PNEC), we have estimated the maximum allowable values in the 2 environment for these chemicals to be 1.093 mg L -1 for levulinic acid, 2.761 mg L -1 for methyl levulinate, 0.982 mg L -1 for ethyl levulinate and 0.151 mg L -1 for butyl levulinate.

show abstract

Section: Levulinate Toxicity In Algaementioning

confidence: 99%

Ecotoxicity studies of the levulinate ester series

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Podola and Melkonian (2003) also reported that a methanol-induced inhibition of photosynthesis was clearly not the same among different algal species or strains. Juneau et al (2001Juneau et al ( , 2002 observed that the sensitivity of algal species to mercury, cupper and to the herbicide metolachlor also varied largely, according to the used species and toxicants. These authors suggested that morphological features might affect the algal sensibility to chemical substances.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, when using PAM fluorometry the choice of the best parameter to describe the response is also important. Juneau et al (2001Juneau et al ( , 2002 considered the operational PSII quantum yield (Φ' M ) as a useful parameter for plant and phytoplankton bioassays. From our results, it appears that Φ' M and ETR were both sensitive parameters for measuring the toxic effect of DCMU on algae.…”

Section: Discussionmentioning

confidence: 99%

Photosynthetic capacity of three phytoplanktonic species measured by a pulse amplitude fluorometric method

Figueredo

Giani

Filho

2009

Braz. J. Plant Physiol.

View full text Add to dashboard Cite

During photosynthesis, absorbed energy that is not used in photochemical reactions dissipates as fluorescence. Fluorescence provides important information on the physiological conditions of the studied organisms and its measurement is widely used by plant physiologists and can be valuable in phytoplankton studies. We describe a method adapting a plant fluorometric equipment to measure the photosynthetic capacity of microalgae. Unialgal cultures of three planktonic chlorophytes were exposed to 3(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), an inhibitor of photosystem II, at concentrations of 0.1, 1.0 and 10.0 µmol.L -1. Estimates were made of photosynthetic parameters, including operational and potential photosystem II quantum yield and electron transport rate between photosystems, using algal cells concentrated on glass-fiber filters. The technique allowed reliable measurements of fluorescence, and detection of distinct levels of inhibition. Physiological or morphological characteristics of the selected species might provide an explanation for the observed results: differences on the surface/volume ratio of the cells and colony morphology, for example, were associated with contrasting resistance to the toxicant. To characterize inhibition on phytoplanktonic photosynthesis, we suggest operational quantum yield and electron transport rate as best parameters, once they were more sensitive to the DCMU toxicity.Key words: chlorophytes photosynthesis, Electron Transport Rate, phytoplankton. resUMoDurante os eventos fotossintéticos, a energia absorvida que não é usada em reações fotoquímicas pode ser dissipada como fluorescência. Sua medida fornece importantes informações acerca das condições fisiológicas dos organismos, sendo amplamente utilizada na fisiologia vegetal e podendo ser valiosa nos estudos do fitoplâncton. Este estudo descreve um método no qual um equipamento elaborado para a realização de medidas de fluorescência em plantas é utilizado para medir a capacidade fotossintética de microalgas. Culturas unialgais de três clorófitas planctônicas foram expostas a três concentrações de 3(3,4-diclorofenil)-1,1-dimetiluréia (DCMU), um inibidor do fotossistema II. Foram quantificados alguns parâmetros fotossintéticos, como os rendimentos quânticos efetivo e potencial do fotossistema II e a taxa de transporte de elétrons entre os fotossistemas. Para tal, as células algais foram concentradas em filtros de fibra de vidro. Esta técnica permitiu a quantificação da fluorescência proveniente do aparato fotossintético destas microalgas, possibilitando a detecção de diferentes níveis de inibição, segundo a espécie. Características fisiológicas ou morfológicas das espécies poderiam justificar os resultados observados: diferenças na razão superfície/volume das células e na forma das colônias, por exemplo, foram associadas aos níveis distintos de resistência ao DCMU. Para a avaliação da inibição da fotossíntese fitoplanctônica, nós sugerimos o rendimento quântico efetivo e a taxa de transporte de elétrons como os parâmet...

show abstract

“…[56] Hg II was also shown to substitute the Mg in chlorophyll molecules [57] and to inhibit the dark reduction of plastoquinone. [58] Nanomolar concentrations of Hg II were found to affect the photosystem of six microalgal species, [59] suggesting that alteration of photosynthesis machinery might be a plausible mechanism of Hg toxicity. However its significance for CH 3 Hg is still to be proved.…”

Section: Mercury Intracellular Transformationsmentioning

confidence: 99%

Towards Mechanistic Understanding of Mercury Availability and Toxicity to Aquatic Primary Producers

Dranguet¹,

Flück²,

Regier³

et al. 2014

Chimia

View full text Add to dashboard Cite

The present article reviews current knowledge and recent progress on the bioavailability and toxicity of mercury to aquatic primary producers. Mercury is a ubiquitous toxic trace element of global concern. At the base of the food web, primary producers are central for mercury incorporation into the food web. Here, the emphasis is on key, but still poorly understood, processes governing the interactions between mercury species and phytoplankton, and macrophytes, two representatives of primary producers. Mass transfer to biota surface, adsorption to cell wall, internalization and release from cells, as well as underlying toxicity mechanisms of both inorganic mercury and methylmercury are discussed critically. In addition, the intracellular distribution and transformation processes, their importance for mercury toxicity, species-sensitivity differences and trophic transfer are presented. The mini-review is illustrated with examples of our own research.

show abstract

Evaluation of different algal species sensitivity to mercury and metolachlor by PAM-fluorometry

Cited by 106 publications

References 25 publications

Ecotoxicity studies of the levulinate ester series

Ecotoxicity studies of the levulinate ester series

Photosynthetic capacity of three phytoplanktonic species measured by a pulse amplitude fluorometric method

Towards Mechanistic Understanding of Mercury Availability and Toxicity to Aquatic Primary Producers

Contact Info

Product

Resources

About