Evaluation of different dietary n ‐3 lc‐pufa on the growth, intestinal health and microbiota profile of golden pompano ( Trachinotus ovatus )

Abstract

The omega‐3 long‐chain polyunsaturated fatty acids (n‐3 LC‐PUFA), such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), are essential fatty acids for marine fish. This study aimed to evaluate different dietary n‐3 LC‐PUFA on the growth, intestinal health and microbiome in carnivorous marine fish Trachinotus ovatus. Three diets, respectively, supplemented with fish oil, EPA‐enriched oil and DHA‐enriched oil were fed juvenile T. ovatus for 10 weeks. The results showed that the three groups showed no difference in the growth, histology and digestive enzyme activities of anterior intestine. Moreover, the intestinal ACP and ALP activities and tnfɑ mRNA level were increased in the EPA‐enriched group, whereas the serum diamine oxidase activity and intestinal lysozyme activity and malondialdehyde content were decreased in both EPA‐ and DHA‐enriched groups. Meanwhile, these two diets also down‐regulated the mRNA expression of Mucin 2, Mucin 13, hes1‐b, Krueppel‐like factor 4 and Occludin, and up‐regulated the zonula occludens‐1 mRNA levels in intestine. Moreover, the two groups significantly decreased the intestinal Vibrio abundance and increased the probiotic proportions, especially in the EPA‐enriched group. These results indicate dietary n‐3 LC‐PUFA would facilitate normal intestinal function and probiotics profiles of T. ovatus, and the EPA‐enriched group appears to be more positive.

Fishmeal Protein Replacement by Defatted and Full-Fat Black Soldier Fly Larvae Meal in Juvenile Turbot Diet: Effects on the Growth Performance and Intestinal Microbiota

“…According to previous studies, insufficient DHA and EPA could impair the development of fish [ 46 – 49 ]. In addition, it has been reported that n-3 long-chain-polyunsaturated fatty acids (mainly DHA and EPA) could enhance intestinal immune functions and positively shape the host microbial ecosystem [ 50 , 51 ]. The DHA and EPA contents in the DF group were higher compared to the FF group in this study.…”

Aurantiochytrium sp. Meal Improved Body Fatty Acid Profile and Morphophysiology in Nile Tilapia Reared at Low Temperature

“…However, in fish fed the highest AM level (40AM), there was a reduction in the number of goblet cells. A previous study evaluated different dietary sources of n-3 LC-PUFA (fish oil, EPA-enriched oil, and DHA-enriched oil) for the carnivorous marine fish pompano, Trachinotus ovatus, raised at an optimal temperature and reported that high dietary EPA or DHA levels caused a depressed expression of Muc13 mRNA in the intestines [54]. Mucins are the major constituent of the mucous layer and are produced by goblet cells [55].…”