Evaluation of different scarification methods to remove hardseededness in Trifolium subterraneum and Medicago polymorpha accessions of the Spanish base genebank

Martin, Ian; Cuadra, Carlos De la

doi:10.15258/sst.2004.32.3.03

Cited by 20 publications

(14 citation statements)

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“…Sensitivity of five alfalfa cultivars to sulphuric acid was also shown by Tomer and Maguire (1989), where 85% germination was obtained when seeds were treated for 30 minutes. Similarly, with results of our study, Martin and De La Cuadra (2004) reached up to 89% germination of Medicago polymorpha L. seeds acid treated for 15 minutes. Balouchi and Sanavy (2006) also reached 96 % germination of Medicago polymorpha and Medicago rigidula (L.) when seeds were soaked in acid for 10 minutes.…”

Section: Resultssupporting

confidence: 92%

Effect of seed coating on germination of Ciliate Medick [Medicago ciliaris (L.) All.] and Snail Medick [Medicago scutellata (L.) Mill.]

Kir

2019

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A study was conducted in the Seed Technology Laboratory of Department of Field Crops, Pasture and Forage Crops Section, Faculty of Agriculture of Ege University, Turkey. Aim of the study was to remove the seed coat hardness by various treatments of Ciliate and Snail Medick to enhance germination which is significant in terms of agronomical handicaps of sowing small seeded legumes under field conditions. Pre-sowing seed treatments (Control, scarification by sandpaper, acid treatment with 95.0-98.0% H2SO4 for 5-10-15 minutes and soaking in water for 24-48 hours) were practiced on these small seeded legumes. All observations and tests were conducted following ISTA regulations and notifications. Germination of tested seeds highly increased by acid treatment for 10-15 minutes duration, while scarification treatment provided favorable germination rates.

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Section: Resultssupporting

confidence: 92%

Effect of seed coating on germination of Ciliate Medick [Medicago ciliaris (L.) All.] and Snail Medick [Medicago scutellata (L.) Mill.]

Kir

2019

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“…Mechanical processing is also frequently used to interrupt seed dormancy. However, such methods may prove troublesome for production on a large scale; examples include nicking lupin seed coats with a razor blade [48], manually rubbing burclover Medicago polymorpha seeds with sand paper, and shaking seeds of clover Trifolium subterraneum with sand in a laboratory shaker [49]. Germination rates may also be improved by a treatment involving the stimulation of seeds with a magnetic field, however, these techniques are rather costly and not as effective as other known methods of seed scarification [50,51].…”

Section: Resultsmentioning

confidence: 99%

“…Dormancy was successfully broken in seeds of burclover, Medicago polimorpha, and clover, Trifolium subterraneum, following a short-term heat treatment with a blowlamp. As a result, seed germination rate increased from 10 to 79% for burclover, and from 46 to 95% for clover [49]. Thermal scarification may also be performed by immersing seeds in hot water or treating them with liquid nitrogen.…”

Section: Resultsmentioning

confidence: 99%

Biochar as a Stimulator for Germination Capacity in Seeds of Virginia Mallow (Sida hermaphrodita (L.) Rusby)

et al. 2019

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This article presents the findings of a laboratory study investigating the stimulation and conditioning of seeds with biochar and the effects observed in the germination and emergence of Virginia mallow (Sida hermaphrodita (L.) Rusby) seedlings. The study shows that biochar, applied as a conditioner added to water in the process of seed hydration, improves their germination capacity. When the processed plant material was added to water at a rate of 5 g (approx. 1250 seeds) per 100 mL, the rate of germination increased to 45.3%, and was 23.3% higher when compared to the control group, and 7.3% higher than in the seeds hydrated without biochar. The beneficial effects of biochar application were also reflected in the increased mass of Virginia mallow seedlings. The mass of seedlings increased by 73.5% compared to the control sample and by 25.9% compared to the seeds hydrated without biochar. Given the low cost of charcoal applied during the hydro-conditioning process, the material can be recommended as a conditioner in large-scale production of Virginia mallow.

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“…2) Heat scarification by soaking the seeds in a hot-water bath at 100 °C for four minutes and then gradually placing them into cold water overnight (Uzun & Aydin 2004;Can et al 2009). 3) Acid scarification by soaking in sulfuric acid (96 %) for four, eight, 10, 15, 20 and 30 minutes, then soaking overnight in tap water (Martin & Cuadra 2004;Kim et al 2008;Alderete-Chavez et al 2010). 4) Soaking the seeds in tap water for 24 hours.…”

Section: Scarification Methodsmentioning

confidence: 99%

Germination responses of Lycium humile, an extreme halophytic Solanaceae: understanding its distribution in saline mudflats of the southern Puna

et al. 2020

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Although knowledge about halophytic Solanaceae is scarce, it is known that several species within genus Lycium tolerate salinity. Lycium humile grows in highly saline soils in mudflats near saline Andean lakes. This study evaluated the germination responses of L. humile under different scarification methods, photoperiods, temperatures and saline conditions and, simultaneously, tested seedling survival under different iso-osmotic conditions. Dormancy and germination were found to be regulated by interactions with different factors, with the highest germination percentages being obtained by immersion in sulfuric acid, with a temperature of 25 °C and a temperature regime of 5/25 °C, under which seeds were neutrally photoblastic. As osmotic potential of saline solutions decreased, germination also decreased drastically but the seedling survival percentage was higher than 30 % at 600 mM NaCl. No seeds germinated in any of the polyethylene glycol (PEG) solutions and no seedling survival was observed from-1.2 MPa PEG solutions. More than 90 % of seeds incubated in NaCl were able to recover germination after being transferred to distilled water, independently of NaCl treatments. We concluded that the effects of extreme environmental conditions on germination responses and seed tolerance to salinity may determine the occurrence and restricted distribution of L. humile.

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Evaluation of different scarification methods to remove hardseededness in Trifolium subterraneum and Medicago polymorpha accessions of the Spanish base genebank

Cited by 20 publications

References 0 publications

Effect of seed coating on germination of Ciliate Medick [Medicago ciliaris (L.) All.] and Snail Medick [Medicago scutellata (L.) Mill.]

Effect of seed coating on germination of Ciliate Medick [Medicago ciliaris (L.) All.] and Snail Medick [Medicago scutellata (L.) Mill.]

Biochar as a Stimulator for Germination Capacity in Seeds of Virginia Mallow (Sida hermaphrodita (L.) Rusby)

Germination responses of Lycium humile, an extreme halophytic Solanaceae: understanding its distribution in saline mudflats of the southern Puna

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