Evaluation of different strategies for determination of selenomethionine (SeMet) in selenized yeast by asymmetrical flow field flow fractionation coupled to inductively coupled plasma mass spectrometry (AF4-ICP-MS)

Abstract

<p>This manuscript exemplifies the prospective use of asymmetrical flow field flow fractionation (AF4) coupled to ICP-MS as a simple tool for chemical speciation of selemethionine in selenized yeast.</p>

Atomic Spectrometry Update: review of advances in elemental speciation

“…In this line, the use of AF 4 -ICP-MS is presented for rst time for the determination of SeMet in selenised yeast. 165 Selenium was released from samples by using different sample treatment protocols including: extraction with; 4 mol L À1 methane sulfonic acid with and without reux, formic acid, alkaline extraction using SDS, water and UAE (both probe and bath) with water. In order to achieve complete recoveries, the AF 4 membrane pore size and interactions between analyte and membrane were taken into consideration.…”

Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages

“…Three reviews addressing different topics that appear in later sections of this update relate to advances in single-cell ultra-trace analysis, 23 asymmetrical flow field flow coupled to fractionation ICP-MS 24 and consideration of blood Ti concentrations as a marker for orthopaedic implant wear. 25…”

“…The procedure for total As, determined by ICP-MS (with O 2 reaction gas) following microwave-assisted digestion (quartz vessels and 200 °C) with HNO 3 –H 2 O 2 , was validated by the analysis of the Nuclear Chemistry and Technology Institute (Warsaw, Poland) Control Material CS-M-3 (microelements in mushroom powder). Several sample preparation methods were evaluated 24 for the determination of SeMet in selenized yeast by asymmetric flow FFF ICP-MS. The seven procedures were extraction with methanesulfonic acid (with and without reflux step), room temperature extraction with HCOOH, alkaline extraction with SDS, and H 2 O extraction (both manual and ultrasound-assisted).…”