EVALUATION OF DIVERSITY AND GENETIC STRUCTURE AS STRATEGIES FOR CONSERVATION OF NATURAL POPULATIONS OF Dalbergia nigra (Vell.) Allemão ex Benth.

Genetic characterization of remaining populations of paratecoma peroba, an endangered and endemic species of the atlantic forest

“…Compared to another endangered and endemic species of the atlantic forest, called Dalbergia nigra (Silva Júnior et al, 2020), evaluated in forest fragments close to or similar to those in this study, the number of total bands and the percentage of polymorphism were lower to those found for P. peroba. This comparison can also be made with other forest species that do not qualify as vulnerable (Vieira et al, 2018;Vieira et al, 2022), however, which have values similar to those found, showing that the individuals evaluated in the populations of P. peroba maintain high genetic variability.…”

“…Regarding the informativeness of ISSR markers, the analysis of polymorphic information content (PIC) has been performed in several studies with forest species (Silva Júnior et al, 2020;Santos et al, 2021), as they represent the capacity of a marker detect genetic variability among individuals (Preczenhak, 2013). According to Tatikonda et al (2009) the maximum PIC value for dominant markers is 0.5, representing high informativeness, however, there is a variation rate where values from 0 to 0.25 indicate low informativeness, above 0.25 to 0.45 for moderately informative and above 0.45 are already considered highly informative. By this classification, the ISSR markers used in this study are characterized as moderately informative when submitted to individual populations and to joint data (Table 2), showing reliability in the data obtained.…”

“…Among the molecular markers, inter simple sequence repeats (ISSR) stand out, being widely used in genetic studies of forest species (Rajasekharan et al, 2017;Silva Júnior et al, 2020). As the first molecular and population genetics characterization of P. peroba, ISSR markers are useful as no knowledge on the genome sequence of the species is required.…”

Environmental and Plant Variables Influence Dalbergia nigra (Fabaceae) Phenology - Implications for Seed Production

“…The alternation of flowering among plants in the same subpopulation in different years can produce seeds with genetically distinct constitutions, with different parental contributions to gene flow that could avoid endogamic depression. D. nigra individuals in small remnant populations show high genetic variation (Ribeiro et al, 2005;Silva-Júnior et al, 2020), so that the intra-and inter-subpopulation variations of flowering and fructification may be considered strategies to avoid endogamic depression, which will be important for seed production by native Brazilian forests species, especially within a scenario of global climate change. The strategy of alternating flowering in different individuals within a subpopulation must be considered in seed production programs, as harvesting from different plants during different periods could improve access to the genetic diversity of those subpopulations (Huenneke, 1991;Gaudinier & Blackman, 2020).…”

Morphological, anatomical and genetic diversity of Nanhaia speciosa (Champ. ex Benth.) J.Compton & Schrire from Northern Vietnam

“…The amount of alleles present at different genomic locations relies on various environmental conditions. Evaluation of genetic diversity structure helps conserve and construct strategies for exploiting genetic resources (da Silva Júnior et al 2020). Inter-Simple Sequence Repeat (ISSR) and Random Amplified Polymorphic DNA (RAPD) are two molecular biology techniques commonly used separately or combined to assess genetic diversity within or between species, such as Enhalusacoroides (Lf) Royle (Pharmawati et al 2021), Clerodendrum species of North East India (Gogoi et al 2020), Lathyrus species (Osman and Ali 2020), Embelia ribes Burm f. (Kamble et al 2023).…”