Evaluation of DNA and nuclear protein features for their use in describing normal and malignant endometrium

Oud, Peter S.; Katzko, Michael W.; Pahlplatz, M. M. M.; Vooijs, G. P.

doi:10.1002/cyto.990080504

Cited by 11 publications

(8 citation statements)

References 17 publications

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“…In short, it includes selection of the CIN I11 and INV lesions from 50 pm "thick" paraffinembedded tissue sections, using a dissecting microscope. After incubation with 0.1% protease of 37°C for 60 min and centrifugation, the cell suspension is placed on slides and stained with a DNA specific thionin-Feulgen stain (23).…”

Section: Cell Materialsmentioning

confidence: 99%

“…Digitized images offer information on nuclear features such as geometry, density, and texture, which are not perceived on light microscopic evaluation even by well-trained human observers. Cytophotometric analysis was proved to be of value in the study of cervical intermediate cells present in "normal," dysplastic, and carcinoma in situ specimens, in studies of normal and malignant endometrium, in the differential diagnosis in pleural effusions, and in the discrimination between classic and variant type small cell lung cancer cell lines (5,6,17,23,24).…”

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confidence: 99%

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DNA ploidy and cytophotometric analysis of cervical intraepithelial neoplasia grade III and invasive squamous cell carcinoma

1990

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Cervical intraepithelial neoplasia grade I11 (CIN 111) and squamous cell carcinoma (INV) were examined using DNA ploidy and cytophotometric analysis. Based on hysterectomy, exconisation, and biopsy material from 69 patients in two age categories, analysis was performed in nuclei isolated from selected areas of paraffin-embedded tissue. High percentages of DNA-diploidy in INV lesions were found mainly in the group of patients age 45 years or younger. CIN I11 lesions in women age 46 or older demonstrated high percentages of DNAaneuploidy. DNA-polyploidy was most frequent in CIN I11 lesions in the younger age category. The results of cytophotometric analysis indicated that the overall mean values of 16 nuclear photometric features discriminated significantly between the whole groups of CIN I11 (n = 37) and INV (n = 32). On an individual patient level, however, the mean feature values showed a large overlap. Based on the results of a stepwise linear discriminant analysis of patient mean values, a combination of geometrical and runlength texture features was used to discriminate between CIN I11 and INV lesions. The correct classification rate was highest in the category of patients in the older age category. The results of this study indicate age related differences in CIN I11 and invasive squamous cell carcinoma, and they may be of help in assessing cytophotometric features in the study of progressive and non-progressive CIN lesions.Key terms: Age, paraffin-embedded sample, cytospin, progressive potential Cervical intraepithelial neoplasia grades I to I11 (CIN 1-111 = dysplasia and carcinoma in situ) are potentially precancerous lesions. They can progress to invasive squamous cell carcinoma, but can also persist or return to normal. Earlier follow-up studies of light microscopically detected CIN I and I1 lesions have shown varying frequencies of progression, persistence, and regression (8,9,12,13,19,20,26,28). The end point of most follow-up studies of CIN I and I1 was the development of CIN 111 ( = severe dysplasia and carcinoma in situ), at which time the patients were treated or left the study. Data on the follow-up of CIN 111 itself are less frequent but generally indicate that a relatively small number of CIN I11 lesions will eventually progress to a n invasive squamous cell carcinoma (9,12,13,18,19). Light microscopically, CIN I11 can not be subdivided into progressive, persistent, or regressive subtypes, and as a consequence all cases of CIN I11 are treated as essentially premalignant, potentially progressive lesions. An alternative for visual light microscopic evaluation of CIN I11 lesions is the study of nuclear DNA ploidy and cytophotometric analysis of structural nuclear changes. DNA ploidy analysis, using flow cytometric and histometric techniques, has indicated high percentages of DNA-aneuploidy in CIN 111 lesions (10,16). On this basis some authors concluded that DNA-aneuploidy is a marker for progression in CIN lesions (10). In a recent study on 39 patients with CIN 111, with and without synchronous i...

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Section: Cell Materialsmentioning

confidence: 99%

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confidence: 99%

DNA ploidy and cytophotometric analysis of cervical intraepithelial neoplasia grade III and invasive squamous cell carcinoma

1990

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“…When fluorescent reporter molecules are used, the measurements were either direct or delayed (timeresolved fluorescence imaging). There is strong evidence from some of these studies that chromatin shows different patterns according to the cell type (Gauvain et al, 1987;Cowden and Curtis, 1981), the state of cell proliferation (Stokke et al, 1988;Rabinovich et al, 1986;Moser et al, 1981), the state of cell differentiation (Giroud et al, 1988;Darzynkiewicz et al, 1984), the set of transcriptionally active genes (Bottone et al, 1989;Bottiroli et al, 1984), the effects of drugs (Block et al, 1987), and neoplasia (Brugal et al, 1986;Oud et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

Fluorescence image cytometry of nuclear DNA content versus chromatin pattern: a comparative study of ten fluorochromes.

Santisteban

Montmasson²,

Giroud³

et al. 1992

J Histochem Cytochem.

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This study is intended to be the first step of an in situ exploration of the intranuclear DNA distribution by image cytomeuy (SAMBA) with several fluorochromes. The nuclear DNA content and the chromatin pattern, revealed by ten fluorochromes (HO, DAPI, MA, CMA3, OM, QM, AO, EB, PI, and 7-AMD), were analyzed on mouse hepatocytes Fied by the Boehm-Sprenger procedure optimal for preserving the chromatin pattem. The question was whether fluotochromes specific to DNA make it possible to accurately quantitate the total nuclear DNA content when the chromatin pattern is preserved. Only HO and MA were found to provide satisfactory quantitation of nuclear DNA content, as assumed by both a small CV and a 4c to 2c ratio equal to 2. PI, EB, 7-AMD, and OM provided higher CV values, although the 4c to 2c ratio was stiU equal to 2. QM, AO, CMA3, and DAPI provided non-reproducible and non-stoichiometric nuclear DNA content measurements under the Fiation conditions used. The intranuclear and the intemuclear SD of the fluorescence intensities describing the fluorescence pattern of the

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“…To reduce the overall variation associated with mean values of the features, cells from the GO/G1 region of a DNA distribution were selected and used for analysis of variances (14).…”

Section: Discussionmentioning

confidence: 99%

Quantitative description of classic and variant small cell lung cancer cell lines by nuclear image cytometry

et al. 1988

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Six small cell lung cancer (SCLC) cell lines were examined using nuclear image analysis to find features characteristic of the classic and the variant type of SCLC. On the basis of their biochemical and biological properties three of these cell lines have been shown to represent the classic types, and three represent the variant type of SCLC. Using a combination of the image‐derived run length, density, and geometric features, it was possible to distinguish between the classic and variant SCLC cell lines.The results of this study may be of help in assessing photometric features for the separation of the classic and variant subtypes of SCLC in solid tumors. Because of differences in treatment and prognosis between these two subtypes, such a separation may be of clinical value.

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Evaluation of DNA and nuclear protein features for their use in describing normal and malignant endometrium

Cited by 11 publications

References 17 publications

DNA ploidy and cytophotometric analysis of cervical intraepithelial neoplasia grade III and invasive squamous cell carcinoma

DNA ploidy and cytophotometric analysis of cervical intraepithelial neoplasia grade III and invasive squamous cell carcinoma

Fluorescence image cytometry of nuclear DNA content versus chromatin pattern: a comparative study of ten fluorochromes.

Quantitative description of classic and variant small cell lung cancer cell lines by nuclear image cytometry

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