Evaluation of DNA-binding and DNA-photocleavage ability of tetra-cationic porphyrins containing peripheral [Ru(bpy)2Cl]+ complexes: Insights for photodynamic therapy agents

Oliveira, Vanessa A.; Terenzi, Hernán; Menezes, Lucas B.; Chaves, Otávio Augusto; Iglesias, Bernardo A.

doi:10.1016/j.jphotobiol.2020.111991

Cited by 33 publications

(14 citation statements)

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“…CD spectroscopy is an important tool to study the stereoselectivity of DNA-binding of a metal complex in solution. , The CD spectra of Δ -[Ru(bpy) 2 MBIP] 2+ and Λ -[Ru(bpy) 2 MBIP] 2+ in the absence and presence of different amounts of DNA were recorded respectively, and are presented in Figure .…”

Section: Resultsmentioning

confidence: 99%

Comparative Studies on DNA-Binding Mechanisms between Enantiomers of a Polypyridyl Ruthenium(II) Complex

et al. 2022

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A pair of ruthenium(II) complex enantiomers, Δ- and Λ-[Ru(bpy)2MBIP]2+ (bpy = 2,2′-bipyridine, MBIP = 2-(3-bromophenyl)imidazo[5,6-f]phenanthroline), were designed, synthesized, and characterized. Comparative studies between the enantiomers on their binding behaviors to calf thymus DNA (CT-DNA) were conducted using UV−visible, fluorescence, and circular dichroism spectroscopies, viscosity measurements, isothermal titration calorimetry, a photocleavage experiment, and molecular simulation. The experimental results indicated that both the enantiomers spontaneously bound to CT-DNA through intercalation stabilized by the van der Waals force or the hydrogen bond and driven by enthalpy and that Δ-[Ru(bpy)2MBIP]2+ intercalated into DNA more deeply than Λ-[Ru(bpy)2MBIP]2+ did and exhibited a better DNA photocleavage ability. Molecular simulation further indicated that Δ-[Ru(bpy)2MBIP]2+ more preferentially intercalated between the base pairs of CT-DNA to the major groove, and Λ-[Ru(bpy)2MBIP]2+ more favorably intercalated to the minor groove. These research findings should be very helpful to the understanding of the stereoselectivity mechanism of DNA-bindings of metal complexes, and be useful for the design of novel metal-complex-based antitumor drugs with higher efficacy and lower toxicity.

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Section: Resultsmentioning

confidence: 99%

Comparative Studies on DNA-Binding Mechanisms between Enantiomers of a Polypyridyl Ruthenium(II) Complex

et al. 2022

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“…The self-aggregation of porphyrins in solution is very common, mainly due to strong attractive interactions between conjugation structures, but it will reduce the activity of porphyrins . Therefore, we studied aggregation behavior of prepared gallium corroles by UV–vis spectra in DMF and DMF/Tris–HCl (pH 7.2) buffer mixture solution.…”

Section: Resultsmentioning

confidence: 99%

Halogenated Gallium Corroles:DNA Interaction and Photodynamic Antitumor Activity

et al. 2021

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A series of halogenated gallium corroles were synthesized and characterized by UV−vis, HRMS, NMR, and FT-IR. The interaction between these gallium corroles and calf thymus DNA had been investigated by spectroscopic methods. These gallium corroles would interact with CT-DNA via an outside binding mode. The photodynamic antitumor activity in vitro of these gallium corroles toward different cell lines had also been tested. 3-Ga displayed low cytotoxicity to normal cells under both light and dark conditions but high phototoxicity to liver cancer cells HepG2. The vitro experiment results showed that 3-Ga could be efficiently absorbed by tumor cells. After light illumination, it may induce reactive oxygen species (ROS) and cause destruction of the mitochondrial membrane potential, which may finally trigger tumor cell apoptosis. Flow cytometry results showed that HepG2 cells were mainly distributed in the sub-G0 phase, which corresponds to cells with highly fragmented DNA or dead cells generally. This suggests that 3-Ga could lead to tumor cell apoptosis after light illumination.

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“…DNA photocleavage involves production of ROS through activation of a photosensitizer (PS) by visible or near-infrared light [52] , [53] . Fig.…”

Section: Mechanism Of Nucleic Acids Cleavagementioning

confidence: 99%

“…AMSs as metal complexes are considered as ideal PS in PDT due to their in vitro effective DNA photocleavage [52] , [53] . The most important reason is that despite of organic PSs that just the ππ * transitions lead to PDT effects, in the electronic spectrum of metal complexes four types of transitions may be observed which depend on the type of metal and ligands.…”

Section: Therapeutic Applications Of Metalloscissorsmentioning

confidence: 99%

“…A rich library of DNA/RNA–cleaving metal complexes have been prepared and submitted to pharma studies as novel therapeutic candidates for the treatment of diseases such as cancer [13] , [14] , [34] , [35] , [36] , [37] , [38] , [39] , bacterial [13] , [14] , [39] , [40] , [41] , [42] , [43] , [44] , [45] and viral infections [13] , [14] , [46] , [47] , [48] , [49] , and gene therapy [50] , [51] . Moreover, metal complexes are considered as suitable photosensitizers for photodynamic therapy (PDT) of cancer and microbial infections due to their efficient in vitro DNA photocleavage [52] , [53] , [54] , [55] . Meaningful examples will be further discussed in Section 5 .…”

Section: Introductionmentioning

confidence: 99%

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Nuclease-like metalloscissors: Biomimetic candidates for cancer and bacterial and viral infections therapy

Anjomshoa

Amirheidari

2022

Coordination Chemistry Reviews

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Despite the extensive and rapid discovery of modern drugs for treatment of cancer, microbial infections, and viral illnesses; these diseases are still among major global health concerns. To take inspiration from natural nucleases and also the therapeutic potential of metallopeptide antibiotics such as the bleomycin family, artificial metallonucleases with the ability of promoting DNA/RNA cleavage and eventually affecting cellular biological processes can be introduced as a new class of therapeutic candidates. Metal complexes can be considered as one of the main categories of artificial metalloscissors, which can prompt nucleic acid strand scission. Accordingly, biologists, inorganic chemists, and medicinal inorganic chemists worldwide have been designing, synthesizing and evaluating the biological properties of metal complexes as artificial metalloscissors. In this review, we try to highlight the recent studies conducted on the nuclease-like metalloscissors and their potential therapeutic applications. Under the light of the concurrent Covid-19 pandemic, the human need for new therapeutics was highlighted much more than ever before. The nuclease-like metalloscissors with the potential of RNA cleavage of invading viral pathogens hence deserve prime attention.

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Evaluation of DNA-binding and DNA-photocleavage ability of tetra-cationic porphyrins containing peripheral [Ru(bpy)2Cl]+ complexes: Insights for photodynamic therapy agents

Cited by 33 publications

References 100 publications

Comparative Studies on DNA-Binding Mechanisms between Enantiomers of a Polypyridyl Ruthenium(II) Complex

Comparative Studies on DNA-Binding Mechanisms between Enantiomers of a Polypyridyl Ruthenium(II) Complex

Halogenated Gallium Corroles:DNA Interaction and Photodynamic Antitumor Activity

Nuclease-like metalloscissors: Biomimetic candidates for cancer and bacterial and viral infections therapy

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