Evaluation of Dri-Dot OMPs Salmonella Typhi in Suspected Typhoid Fever Patients as an Immunodiagnostic Kit

Abstract

Background. Several studies have identified a protein of OMP S. Typhi. The protein is highly immunogenic and can be recognize whole cells of S. Typhi. The aims of the study was to evaluate the Dri-dot OMPs immunoreactivity in typhoid fever suspected patients. Method. Samples obtained from Dr. Wahidin Sudirohusodo Hospital, lbnu Sina Hospital, and Haji Hospital of Makassar from February to November 2014. Their sensitivity and specificity were evaluated against blood culture method as the gold standard. For each of the two tests, sensitivity, specificity, positive and negative predictive values were calculated using standard formulae. Results. A total of 15 of suspected typhoid fever samples examined for culture, lateral flow and Dri-dot OMPs. Examination of blood culture method showed that 3 of 15 (20%) patients had a blood culture positive for S. typhi. Dri-dot OMPs were positive in 13 (86.7%) serum samples. Fourteen (93.3%) serum samples were positive for lateral flow examination. Thirteen samples were positive for both Dri-dot and lateral flow. One sample was negative for both lateral flow and Dri-dot. One sample was positive for Dri-dot but negative for lateral flow. Conclusion. We conclude that there was no difference between Dri-dot OMPs, lateral flow and widal tests. Dri-dot could be of use for the diagnosis of typhoid fever in patients who have clinical typhoid fever.

TELAAH METODE DIAGNOSIS CEPAT DAN PENGOBATAN INFEKSI <em>Salmonella typhi</em>

“…Jumlah kasus 22.000.000 per tahun di dunia dan mengakibatkan 216.000-600.000 orang menderita demam tifoid. Pada tahun 2002, terjadi 408.837 kasus demam tifoid di Afrika (Gunn et al 2014;Keddy et al 2011 Siba et al 2012;Mangarengi et al 2015;Alba et al 2016;Lamas et al 2018;Purba et al 2016;Yasin et al 2018). Sebanyak 1 sampai 5% orang yang terinfeksi menjadi karier kronis karena menyimpan S. thypi di kandung empedu walaupun telah diobati dengan antibiotik (Huang dan Dupont 2005).…”

“…Demam tifoid merupakan penyakit umum di negara berkembang sehingga diperlukan medote diagnosis yang memadai (Mangarengi et al 2015). Pada umumnya diagnosis awal demam tifoid berdasarkan hasil evaluasi tanda dan gejala klinis (Andrew dan Ryan 2015).…”

“…Gejala demam tifoid berupa demam sebagaimana gejala penyakit infeksi pada umumnya, seperti infeksi malaria, demam berdarah dengue, dan leptosirosis sehingga diagnosis yang tepat menjadi sangat penting. Gejala lain demam tifoid adalah: sakit kepala, sakit otot, menggigil, gangguan pernafasan, sakit perut dan konstipasi atau diare (Tam et al 2008;Mangarengi et al 2015;Li et al 2018;Johnson et al 2018…”