Evaluation of Dried Amniotic Membrane on Wound Healing at Split-Thickness Skin Graft Donor Sites: A Randomized, Placebo-Controlled, Double-blind Trial

Abstract

OBJECTIVE To evaluate the effect of amniotic membrane (AM) at split-thickness skin graft (STSG) donor sites. METHODS This double-blind randomized controlled trial was conducted on 35 eligible participants referred to the burn unit of Vasei Hospital of Sabzevar, Iran, during 2017 and 2018. Each STSG donor site was divided into two sides, and the respective halves were covered with either a dried AM or petrolatum gauze (control). Outcomes were evaluated on postprocedure days 10, 20, and 30 using the Vancouver Scar Scale. RESULTS The mean age of the patients was 39.4 ± 13.97 years, and 62.8% (n = 22) were male. There was no statistically significant difference in wound healing rate on day 10 (P = .261), 20 (P = .214), or 30 (P = .187) between groups. The intervention group had significantly better epithelialization than the control group on day 10 (investigator 1, 1.62 ± 0.59 vs 1.40 ± 0.88 [P = .009); investigator 2, 1.22 ± 0.84 vs 0.91 ± 0.85 [P = .003]), as well as pain reduction (P < .001 during the follow-up period). However, there was no statistically significant difference between groups in terms of pigmentation or vascularization (P > .05). CONCLUSIONS Findings suggest that the use of AM is not superior to petrolatum gauze in terms of STSG healing rate; however, AM achieved better pain reduction and epithelialization on day 10.

Comparison of the Effectiveness of Methods for Obtaining and Application the Stromal-Vascular Fraction of Adipose Tissue in an Experimental Model of a Donor Wound in Burns

“…В современной практике используются различные способы влияния на процессы репаративной регенерации в донорских ранах [20][21][22]. Однако исследований эффективности применения…”

Outcome of Application of Cryopreserved Amniotic Membrane Grafts in the Treatment of Chronic Nonhealing Wounds of Different Origins in Polymorbid Patients: A Prospective Multicenter Study

“…The fact that the application of amniotic or amniochorionic membranes leads to a significant reduction in pain has been repeatedly noted in clinical studies using cryopreserved [ 31 , 51 , 52 ] or air-dried terminally sterilized tissue [ 53 ]. The analgesic effect of cryopreserved AM was mainly explained by the reduction of inflammation and thus secondary to pain relief [ 31 , 54 ].…”

Quantification of Analgesic and Anti-Inflammatory Lipid Mediators in Long-Term Cryopreserved and Freeze-Dried Preserved Human Amniotic Membrane

“…To the best of our knowledge, no study has compared the analgesic effect between multiple types of AM. Of the studies where pain was assessed using a pain score, analgesic effect was verified in studies using fresh [ 16 , 17 ], cryopreserved [ 33 , 62 , 63 ], and air-dried terminally sterilized (gamma irradiation 25 k Gy) [ 34 ] AM allografts for various wound treatment.…”

“…During clinical studies, apart from accelerating healing, the most outstanding effect of AM application is rapid wound pain alleviation [ 17 , 33 , 34 ]. The exact mechanism of analgesic action of AM was not known for a long time and no directly acting component was characterized [ 35 ].…”