Evaluation of E. coli Nissle1917 derived metabolites in modulating key mediator genes of the TLR signaling pathway

Damoogh, Sheyda; Vosough, Mehrad; Hadifar, Shima; Rasoli, Masoumeh; Gorjipour, Ali; Falsafi, Sarvenaz; Behrouzi, Ava

doi:10.1186/s13104-021-05568-x

Cited by 11 publications

(9 citation statements)

References 45 publications

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“…Studies have shown that probiotic have a regulatory role on immune response [ 30 – 32 ] and we think this regulatory role of probiotic may be because of the probiotic effect on pattern recognition receptors (PRRs) molecules such as TLR, whatever the further study need. Noteworthy, TLRs play an crucial role in inducing anti-inflammatory or inflammatory response and subsequent activation of adaptive immune responses such as antibody production [ 33 ]. We recently showed that decreased TLR4 expression and a differently increased TLR2 expression in response to ligand treatment in spermatozoa is related to unexplained RSA [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

The effect of probiotics on immunogenicity of spermatozoa in couples suffering from recurrent spontaneous abortion

et al. 2022

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Background Impaired spermatozoa immunogenicity can result in pregnancy complications such as recurrent spontaneous abortion (RSA). Given that spermatozoa contact with microbiota, it is possible that inappropriate microbiota composition in the reproductive tract could result in the alteration of spermatozoa antigenicity. Probiotics, as a representative of microbiota, may therefore have a beneficial effect on this altered immunogenicity. The objective of this study was to determine the effect of probiotics on spermatozoa immunogenicity. Methods Twenty-five fertile couples and twenty-five RSA couples were included in this study. Spermatozoa were purified and treated with probiotics. Untreated and probiotic treated spermatozoa were evaluated for human leukocyte antigen (HLA) class I & II expression by flow cytometry. Untreated and probiotic treated spermatozoa were also cocultured with the wife’s peripheral blood mononuclear cells (PBMC) for 12 days. Then, the supernatant was assessed for IgG and APCA by enzyme-linked immunosorbent assay (ELISA) and complement-dependent cytotoxicity (CDC) assay respectively. Results Probiotic treatment of spermatozoa leads to an increase of HLA class I & II expression in both the fertile and RSA groups. The probiotic treatment resulted in a decrease in both IgG and APCA in the fertile group, but an increase in both IgG and APCA in the RSA group. Conclusions The results of this study suggest that a supplementary probiotic treatment may be useful in couples suffering from RSA with an immunologic cause, because it improves disturbed HLA expression on spermatozoa and improves disturbed APCA and IgG production in the presence of spermatozoa.

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Section: Discussionmentioning

confidence: 99%

The effect of probiotics on immunogenicity of spermatozoa in couples suffering from recurrent spontaneous abortion

et al. 2022

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“…In vitro models of IBD are increasingly being used in research on the involvement of TLR (toll-like receptor) in the IBD. Several researchers have used HT29 cell lines to conduct recent studies on TLR receptors [ 43 – 47 ].…”

Section: In Vitro Modelsmentioning

confidence: 99%

In vitro models and ex vivo systems used in inflammatory bowel disease

Joshi¹,

Soni²,

Acharya³

2022

In vitro models

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Inflammatory bowel disease (IBD) is a chronic, relapsing gastrointestinal condition. Ulcerative colitis and Crohn’s disease are types of inflammatory bowel disease. Over many decades, the disease has been a topic of study, with experts still trying to figure out its cause and pathology. Researchers have established many in vivo animal models, in vitro cell lines, and ex vivo systems to understand its cause ultimately and adequately identify a therapy. However, in vivo animal models cannot be regarded as good models for studying IBD since they cannot completely simulate the disease. Furthermore, because species differences are a crucial subject of concern, in vitro cell lines and ex vivo systems can be employed to recreate the condition properly. In vitro models serve as the starting point for biological and medical research. Ex vivo and in vitro models for replicating gut physiology have been developed. This review aims to present a clear understanding of several in vitro and ex vivo models of IBD and provide insights into their benefits and limits and their value in understanding intestinal physiology.

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“…Recently, a novel postbiotic from Lactobacillus rhamnosus could show beneficial effects on intestinal barrier function and also potential protection of liver injury ( 200 ). Another recently published study evaluated the anti-inflammatory effects of E. coli Nissle1917 derived metabolites and found TNF-α production as well as TLR signaling pathways to be reduced ( 201 ).…”

Section: Therapeutic Interventions Targeting the Gut-liver-axismentioning

confidence: 99%

“…Another recently published study evaluated the antiinflammatory effects of E. coli Nissle1917 derived metabolites and found TNF-α production as well as TLR signaling pathways to be reduced (201).…”

Section: Alternative Approaches Under Developmentmentioning

confidence: 99%

Molecular and Cellular Mediators of the Gut-Liver Axis in the Progression of Liver Diseases

et al. 2021

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The gut-liver axis covers the bidirectional communication between the gut and the liver, and thus includes signals from liver-to-gut (e.g., bile acids, immunoglobulins) and from gut-to-liver (e.g., nutrients, microbiota-derived products, and recirculating bile acids). In a healthy individual, liver homeostasis is tightly controlled by the mostly tolerogenic liver resident macrophages, the Kupffer cells, capturing the gut-derived antigens from the blood circulation. However, disturbances of the gut-liver axis have been associated to the progression of varying chronic liver diseases, such as non-alcoholic fatty liver disease, non-alcoholic steatohepatitis, and primary sclerosing cholangitis. Notably, changes of the gut microbiome, or intestinal dysbiosis, combined with increased intestinal permeability, leads to the translocation of gut-derived bacteria or their metabolites into the portal vein. In the context of concomitant or subsequent liver inflammation, the liver is then infiltrated by responsive immune cells (e.g., monocytes, neutrophils, lymphoid, or dendritic cells), and microbiota-derived products may provoke or exacerbate innate immune responses, hence perpetuating liver inflammation and fibrosis, and potentiating the risks of developing cirrhosis. Similarly, food derived antigens, bile acids, danger-, and pathogen-associated molecular patterns are able to reshape the liver immune microenvironment. Immune cell intracellular signaling components, such as inflammasome activation, toll-like receptor or nucleotide-binding oligomerization domain-like receptors signaling, are potent targets of interest for the modulation of the immune response. This review describes the current understanding of the cellular landscape and molecular pathways involved in the gut-liver axis and implicated in chronic liver disease progression. We also provide an overview of innovative therapeutic approaches and current clinical trials aiming at targeting the gut-liver axis for the treatment of patients with chronic liver and/or intestinal diseases.

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Evaluation of E. coli Nissle1917 derived metabolites in modulating key mediator genes of the TLR signaling pathway

Cited by 11 publications

References 45 publications

The effect of probiotics on immunogenicity of spermatozoa in couples suffering from recurrent spontaneous abortion

The effect of probiotics on immunogenicity of spermatozoa in couples suffering from recurrent spontaneous abortion

In vitro models and ex vivo systems used in inflammatory bowel disease

Molecular and Cellular Mediators of the Gut-Liver Axis in the Progression of Liver Diseases

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