Evaluation of early healing events around mesenchymal stem cell-seeded collagen–glycosaminoglycan scaffold. An experimental study in Wistar rats

The development of non-viral gene-activated matrices for bone regeneration using polyethyleneimine (PEI) and collagen-based scaffolds

“…The beneficial effects of nHa incorporation include improved osteointegration, increased mechanical properties and enhanced cell function [39][40][41]. In addition, the excellent capacity of these scaffolds to repair and regenerate bone defects has been exhibited in a number of in vivo models [41][42][43]. However, in order to heal very large defects such as those which typically require bone grafting in humans, an extra agent, i.e.…”

Addition of hyaluronic acid improves cellular infiltration and promotes early-stage chondrogenesis in a collagen-based scaffold for cartilage tissue engineering

“…Due to the highly porous nature of the scaffolds used in this study, we believe that the cell density was sufficient to investigate the interaction of cells and scaffolds. This cell seeding density was based on previous work carried out in our laboratory and has been used both in vitro and in vivo (Farrell et al, 2006, Byrne et al, 2008, Lyons et al, 2010b, Alhag et al, 2011. A higher cell density would create a phenomenon whereby cell-cell interaction out-weighed cell-matrix interaction resulting in an imprecise interpretation of the response of MSCs to the differing compositions.…”

Evaluation of the ability of collagen–glycosaminoglycan scaffolds with or without mesenchymal stem cells to heal bone defects in Wistar rats

“…In a previously published work we demonstrated the tissue compatibility and the ability of cell-free CG to provide a scaffold which allows infiltration of host cells [24]. Its full mineralization after 12 weeks of implantation is a further proof of its osteoconductive properties.…”