Evaluation of Echinococcus granulosus recombinant EgAgB8/1, EgAgB8/2 and EPC1 antigens in the diagnosis of cystic echinococcosis in buffaloes

Rialch, Ajayta; Raina, O.K.; Tigga, M. N.; Arun, A. B.; Ganaie, Zamir Ali; Aftab, Andleeb; Lalrinkima, H.; Singh, Mahavir; Varghese, Anju; Samanta, S.; Banerjee, P. S.; Singh, Praveen; Verma, Med Ram

doi:10.1016/j.vetpar.2018.01.020

Cited by 9 publications

(4 citation statements)

References 28 publications

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“…In previous study, recombinant EPC1 showed sensitivity of 92.2% and specificity of 95.6% in immunoblotting in detection of CE patient serum (Li et al, 2003). In contrast, recombinant EPC1 reacted fairly with sera from infected goats and buffaloes with sensitivity of 60 and 89.3%, respectively, meanwhile with specificity of 72.3% and 51.5% (Rialch et al, 2017(Rialch et al, , 2018. P-29, a 29-kDa antigen from E. granulosus, was first described by González Muzio et al (2000); subsequently, recombinant P29 has been used as a biomarker for posttherapy CE patients (Margutti et al, 2008).…”

Section: Discussionmentioning

confidence: 86%

Preliminary Evaluation of Recombinant EPC1 and TPx for Serological Diagnosis of Animal Cystic Echinococcosis

Liu

Song

et al. 2020

Front. Cell. Infect. Microbiol.

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Animal cystic echinococcosis (CE) is one of the most important helminthic diseases and affects many mammalian intermediate hosts. Practical and effective diagnosis is crucial for animal CE control. Two different recombinant antigens derived from Echinococcus granulosus, Echinococcus protoscolex calcium binding protein 1 (rEg-EPC1) and thioredoxin peroxidase (rEg-TPx), were evaluated in this study to detect the specific immunoglobulin G (IgG) in sheep and goat with CE by the indirect enzyme-linked immunosorbent assays. The diagnostic effect of the above-listed proteins was determined to their sensitivity and specificity and compared with hydatid cyst fluid, two previously reported immunogenic recombinant proteins (dihydrofolate reductase and P29), and two commercial kits available in China. Of these, the best diagnostic results were obtained in the anti-TPx IgG ELISA, with 92.6% sensitivity, 98.8% specificity, and no cross-reactivity with anti-Eg95 IgG. Recombinant E. granulosus thioredoxin peroxidase shows good potential for serological diagnosis of animal cystic echinococcosis.

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Section: Discussionmentioning

confidence: 86%

Preliminary Evaluation of Recombinant EPC1 and TPx for Serological Diagnosis of Animal Cystic Echinococcosis

Liu

Song

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Parasites are capable of causing acute, chronic, and debilitating types of diseases, leading to production losses in animals ( 11 – 15 ), and many pieces of research have shown the positive role of alternative/complementary medicine in treating parasitic diseases. Echinococcosis is a neglected silent cyclozoonotic parasitic disease caused by the metacestode stages of the genus Echinococcus belonging to the family Taeniidae, affecting a wide spectrum of animal species, including livestock and wildlife, and it also has zoonotic implications ( 16 – 19 ). The genus Echinococcus contains at least nine valid species with different strains and genotypes, namely E. granulosus, E. multilocularis, E. vogeli, E. oligarthra, E. canadensis, E. equinus, E. felidis, E. shiquicus , and E. ortleppi , which are important ones ( 20 , 21 ).…”

Section: Introductionmentioning

confidence: 99%

Food-borne zoonotic echinococcosis: A review with special focus on epidemiology

Alvi

Alsayeqh

2022

Front. Vet. Sci.

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Echinococcosis is a neglected, WHO-listed cyclozoonotic parasitic disease that is caused by a number of species belonging to the genus Echinococcus. This disease is widespread across the globe, resulting in heavy economic losses for farmers and cystic disease in aberrant human hosts. This review paper briefly discussed taxonomy, a brief history, the magnitude of economic losses, host spectrum and life cycle, risk factors, and clinical manifestations. Furthermore, the copro- and sero-ELISA-based prevalence of echinococcosis on different continents was summarized. Finally, the authors analyzed the frequency and use of molecular epidemiology in the taxonomy of Echinococcus species based on molecular markers. This review will serve as a quick reference to Echinococcus.

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“…Echinococcosis is a neglected silent cyclozoonotic parasitic disease, caused by metacestodal stages of organisms of genus Echinococcus, belonging to family Taeniidae. These parasites affect wide spectrum of animal species including livestock and wildlife, and also possess zoonotic implications (Singh et al 2012;Sadjjadi et al 2013;Ma et al, 2015;Rialch et al 2018). At present, there is a huge debate over taxonomy of genus Echinococcus at the species level.…”

Section: Introductionmentioning

confidence: 99%

Introduction To Echinococcosis and A Review of Treatment Panels

2021

Int J Vet Sci

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Evaluation of Echinococcus granulosus recombinant EgAgB8/1, EgAgB8/2 and EPC1 antigens in the diagnosis of cystic echinococcosis in buffaloes

Cited by 9 publications

References 28 publications

Preliminary Evaluation of Recombinant EPC1 and TPx for Serological Diagnosis of Animal Cystic Echinococcosis

Preliminary Evaluation of Recombinant EPC1 and TPx for Serological Diagnosis of Animal Cystic Echinococcosis

Food-borne zoonotic echinococcosis: A review with special focus on epidemiology

Introduction To Echinococcosis and A Review of Treatment Panels

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