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Aim:The objective of this study was to determine the prevalence of snail's intermediate host affected with larval stages of different trematodes.Materials and Methods: Snails were collected from ponds, tanks, ditches, canals and crop fields in and around Ranchi. They were transported to the laboratory in water containers and maintained in small aquaria, supplemented with natural food stuff. Then, snails were screened randomly for their infection with different trematodes by cercarial shedding method.Result: A total of 600 snails of different species mainly Indoplanorbis, Gyraulus, Lymnaea spp. and Vivipara were screened by cercarial shedding method out of which 44 (7.33%) were found positive for different trematodes cercariae. The percentage of infection in Indoplanorbis spp., Lymneae spp. and Gyraulus spp. were 7.22%, 8.60%, and 14.67%, respectively. Prevalence was higher in Gyraulus whereas, Vivipara did not show any infection with trematodes cercariae. Conclusion:The present study reveals that Indoplanorbis spp., Lymnaea spp. and Gyraulus spp. are common snails found in and around Ranchi. These snails act as intermediate hosts having infective stages of parasites.

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Echinococcus granulosus recombinant proteins including two antigen B sub-units EgAgB8/1 and EgAgB8/2 and Echinococcus protoscolex calcium binding protein (EPC1) were expressed in prokaryotic expression vectors. The diagnostic potential of these three recombinant proteins was evaluated in the detection of cystic echinococcosis in goats in IgG-ELISA. The EgAgB8/1 and EgAgB8/2 recombinant proteins reacted fairly with the hydatid infected goats with sensitivity of 66.7% and 80.0% and specificity of 71.3% and 73.3%, respectively while EPC1 recombinant protein showed lower sensitivity (60%) but comparable specificity (72.3%). Cross-reactivity of these three antigens with goat gastro-intestinal strongyle nematodes and Taenia hydatigena under field conditions was studied. Results showed that EgAgB8/1, EgAgB8/2 and EPC1 antigens cross-reacted with most of the parasites in the goat host.

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