Evaluation of embryotoxic and embryostatic effects of the aqueous extract of Rhizophora mangle and tannic acid on eggs and larvae of Aedes aegypti

Abstract

Rhizophora mangle is an abundant plant in mangroves and tannic acid is a polyphenol produced by the secondary metabolism of plants. The aim of the study was to evaluate the embryotoxic and embriostatic effects of the aqueous extract of R. mangle and synthetic tannic acid on eggs and larvae of Aedes aegypti. A. aegypti eggs were exposed in duplicate at concentrations of 250, 500, 750 and 1000 µg/mL of extract and tannic acid for a period of 14 days. Mineral water was used as a negative control. The eggs were observed and counted in a stereomicroscope (1.2x). In all extract concentrations there was stimulation in hatching in relation to the control, but only in concentration of 750 mg/mL it was statistically significant. In tannic acid (250µg/ml) there was significant stimulus in hatching, but in 500, 750 and 1000 µg/mL there was significant inhibition. All concentrations of aqueous extract and tannic acid on larvae showed embryotoxic and embryostatic effects when compared to the control. The aqueous extract of R. mangle showed effect on hatching of A. aegypti eggs and synthetic tannic acid showed embryotoxic and embryostatic effects. On larvae, both the aqueous extract as tannic acid showed embryotoxic and embryostatic effects.

Seasonal incidence of pomegranate butterfly, Deudorix livia (Klug) (Lepidoptera: Lycaenidae) on pomegranate and evaluation of susceptibility degrees of pomegranate varieties to infestation by the pest

“…0.081% while in Wonderfull recorded 0.099 % as well the percentage of phenolic compounds recorded 0.028 and0.041%, respectively for Manfaloty and Wonderfull. (Ageu et al, 2018) mentioned that polyphenolic compounds and tannins have toxic effects on the egg hatchability of Aedes aegypti. Also, Soliman (2020) found higher concentrations of phenolic compounds and tannins in the pomegranate variety of Baladi (67.60 and 314.4) 2022) demonstrated that pomegranate cultivars differed in the percentages of total soluble solids, TSS/acidity ratio, total sugars, and total reducing sugars.…”

Larvicidal Activity of Ketapang Leaf Fraction (Terminalia catappa L) on Aedes aegypti Instar III

“…The mechanisms resemble the processes of insects encountering toxic substances. When tannic acid is utilised against Diptera larvae, histopathological processes initially occurs in the anterior region of the midgut, progressing to the median and posterior regions [23].…”

The Impact of Climate Change on the Ecosystem Services of Rivers

“…(2022) sugirieron un panel de miR18a/21/125b circulante como un biomarcador potencial para la detección temprana de GC. Al realizar el análisis de 21 estudios realizados en muestras de plasma y suero de pacientes con CG en diferentes…”