Evaluation of Embryotoxicity Using the Zebrafish Model

Truong, Lisa; Harper, Stacey L.; Tanguay, Robert L.

doi:10.1007/978-1-60761-849-2_16

Cited by 219 publications

(178 citation statements)

References 11 publications

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“…As a test animal, the zebrafish has several advantages, including the fact that this fish is small in size (about 2.5-4 cm), does not need plenty rearing space, has similar morphological and physiological structures to mammals, has external fertilization, and short life cycle. The embryo of this fish is transparent making direct observation in vivo on organ and tissue development possible [9]. In addition, the permeable embryo of zebrafish allows the distribution of active substances into the embryo [10] so that it can be used in a toxicity test.…”

Section: Zebrafish (Danio Rerio)mentioning

confidence: 99%

CURCUMINOID AND TOXICITY LEVELS OF ETHANOL EXTRACT OF JAVANESE GINGER (Curcuma xanthorriza) ON BRINE SHRIMP (Artemia salina) LARVAE AND ZEBRAFISH (Danio rerio) EMBRYOS

Syahbirin

Mumuh

Mohamad

2017

Asian J Pharm Clin Res

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Objective: This study was aimed at determining the levels of curcuminoids and analyzing the toxicity of ethanol extracts of Javanese ginger.Methods: Curcuminoid levels were determined using high-performance liquid chromatography, while the toxicity tests were done on larva of brine shrimp (Artemia salina) by using a brine shrimp lethality test (BSLT) method and embryos of zebrafish (Danio rerio) using a zebrafish embryo acute toxicity (ZFET) method. Results:The level curcuminoid of ethanol extracts was 10.5% dry wt., consisting of curcumin at the highest percentage (68.06%) followed by desmethoxycurcumin (24.6%) and bisdemethoxycurcumin (1.41%). In BSLT method, the lethal concentration 50% values (LC 50 ) value of our ethanol extract was 238 ppm, whereas in ZFET method, the LC 50 value at 96 hours after fertilization was 80 ppm. The ethanol extract of ginger caused major malformations of the pericardial edema of zebrafish embryos at a concentration of 100 ppm. Conclusion:The ethanol extract of Curcuma xanthorrhiza from Bogor contained curcuminoids consisting of curcumin, desmethoxycurcumin, and bisdemethoxycurcumin, with acute toxicity, caused major malformations on the pericardial edema in zebrafish embryos.

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Section: Zebrafish (Danio Rerio)mentioning

confidence: 99%

CURCUMINOID AND TOXICITY LEVELS OF ETHANOL EXTRACT OF JAVANESE GINGER (Curcuma xanthorriza) ON BRINE SHRIMP (Artemia salina) LARVAE AND ZEBRAFISH (Danio rerio) EMBRYOS

Syahbirin

Mumuh

Mohamad

2017

Asian J Pharm Clin Res

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“…At 120 hpf, embryos were assessed for mortality, as well as behavioral, and physical malformations such as: axis, brain, circulation, eye, caudal fin, pectoral fin, jaw, otic, pigment, pericardial edema, yolk sac edema, snout, swim bladder, trunk, somite, and a touch response according to previously published methods. 32,33 Data for individual endpoints analyzed here are freely available on the open-source Nanomaterial-Biological Interactions Knowledgebase (http://www.nbi.oregonstate.edu).…”

Section: Zebrafish Exposurementioning

confidence: 99%

Comparative toxicological assessment of PAMAM and thiophosphoryl dendrimers using embryonic zebrafish

Pryor¹,

Harper²,

Harper³

2014

IJN

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Dendrimers are well-defined, polymeric nanomaterials currently being investigated for biomedical applications such as medical imaging, gene therapy, and tissue targeted therapy. Initially, higher generation (size) dendrimers were of interest because of their drug carrying capacity. However, increased generation was associated with increased toxicity. The majority of studies exploring dendrimer toxicity have focused on a small range of materials using cell culture methods, with few studies investigating the toxicity across a wide range of materials in vivo. The objective of the present study was to investigate the role of surface charge and generation in dendrimer toxicity using embryonic zebrafish ( Danio rerio ) as a model vertebrate. Due to the generational and charge effects observed at the cellular level, higher generation cationic dendrimers were hypothesized to be more toxic than lower generation anionic or neutral dendrimers with the same core composition. Polyamidoamine (PAMAM) dendrimers elicited significant morbidity and mortality as generation was decreased. No significant adverse effects were observed from the suite of thiophosphoryl dendrimers studied. Exposure to ≥50 ppm cationic PAMAM dendrimers G3-amine, G4-amine, G5-amine, and G6-amine caused 100% mortality by 24 hours post-fertilization. Cationic PAMAM G6-amine at 250 ppm was found to be statistically more toxic than both neutral PAMAM G6-amidoethanol and anionic PAMAM G6-succinamic acid at the same concentration. The toxicity observed within the suite of varying dendrimers provides evidence that surface charge may be the best indicator of dendrimer toxicity. Dendrimer class and generation are other potential contributors to the toxicity of dendrimers. Further studies are required to better understand the relative role each plays in driving the toxicity of dendrimers. To the best of our knowledge, this is the first in vivo study to address such a broad range of dendrimers.

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“…At 24 hpf, embryos were assessed for 4 developmental toxicity endpoints (MO24: mortality at 24 hpf, DP: developmental progression, SM: spontaneous movement, and NC: notochord distortion) [24]. At 120 hpf, 17 developmental endpoints were assessed (Table 1) [24].…”

Section: Methodsmentioning

confidence: 99%

Ecotoxicity of the insensitive munitions compound 3-nitro-1,2,4-triazol-5-one (NTO) and its reduced metabolite 3-amino-1,2,4-triazol-5-one (ATO)

Madeira

Field

Simonich

et al. 2018

Journal of Hazardous Materials

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The insensitive munitions compound 3-nitro-1,2,4-triazol-5-one (NTO) was recently approved by the U.S. Army to replace cyclotrimethylene trinitramine (RDX) in conventional explosives. As its use becomes widespread, concern about the potential toxicity of NTO increases. NTO can undergo microbial reduction to 3-amino-1,2,4-triazol-5-one (ATO), which is recalcitrant in waterlogged soils. In this study, the acute toxicity of NTO and ATO towards various organisms, including microorganisms (i.e., methanogenic archaea, aerobic heterotrophs, and Aliivibrio fischeri (Microtox assay)), the microcrustacean Daphnia magna (ATO only), and zebrafish embryos (Danio rerio), was assessed. NTO was notably more inhibitory to methanogens than ATO (IC50=1.2 mM, >62.8 mM, respectively). NTO and ATO did not cause noteworthy inhibition on aerobic heterotrophs even at the highest concentrations tested (32.0 mM). High concentrations of both NTO and ATO were required to inhibit A. fischeri (IC20 = 19.2, 22.4 mM, respectively). D. magna was sensitive to ATO (LC50= 0.27 mM). Exposure of zebrafish embryos to NTO or ATO (750 µM) did not cause lethal or developmental effects (22 endpoints tested). However, both compounds led to swimming behavior abnormalities at low concentrations (7.5 µM). The results indicate that the reductive biotransformation of NTO could enhance or lower its toxicity according to the target organism.

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Evaluation of Embryotoxicity Using the Zebrafish Model

Cited by 219 publications

References 11 publications

CURCUMINOID AND TOXICITY LEVELS OF ETHANOL EXTRACT OF JAVANESE GINGER (Curcuma xanthorriza) ON BRINE SHRIMP (Artemia salina) LARVAE AND ZEBRAFISH (Danio rerio) EMBRYOS

CURCUMINOID AND TOXICITY LEVELS OF ETHANOL EXTRACT OF JAVANESE GINGER (Curcuma xanthorriza) ON BRINE SHRIMP (Artemia salina) LARVAE AND ZEBRAFISH (Danio rerio) EMBRYOS

Comparative toxicological assessment of PAMAM and thiophosphoryl dendrimers using embryonic zebrafish

Ecotoxicity of the insensitive munitions compound 3-nitro-1,2,4-triazol-5-one (NTO) and its reduced metabolite 3-amino-1,2,4-triazol-5-one (ATO)

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