Evaluation of enhanced darkfield microscopy and hyperspectral analysis to analyse the fate of silver nanoparticles in wastewaters

Théoret, Trevor; Wilkinson, Kevin J.

doi:10.1039/c7ay00615b

Cited by 27 publications

(17 citation statements)

References 33 publications

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“…The unique interaction between light and matter generates characteristic profiles of scattered light, serving as spectral signatures for each target. Dark field illumination based on an annular cardioid condenser and highly collimated light at oblique angles on the sample, generates images with enhanced contrast and signal-to-noise ratio up to ten times (×10) higher than the conventional dark field optics [ 6 , 7 ]. The instrumentation of the system consists of a visible-near infrared (vis-NIR) spectrophotometer attached to a charge-coupled device (CCD) camera connected with the imaging port of the microscope.…”

Section: Hyperspectral-enhanced Dark Field Microscopy (Hedfm)mentioning

confidence: 99%

Hyperspectral-Enhanced Dark Field Microscopy for Single and Collective Nanoparticle Characterization in Biological Environments

Zamora-Pérez

Tsoutsi

et al. 2018

Materials

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We review how the hyperspectral dark field analysis gives us quantitative insights into the manner that different nanoscale materials interact with their environment and how this relationship is directly expressed in an optical readout. We engage classification tools to identify dominant spectral signatures within a scene or to qualitatively characterize nanoparticles individually or in populations based on their composition and morphology. Moreover, we follow up the morphological evolution of nanoparticles over time and in different biological environments to better understand and establish a link between the observed nanoparticles’ changes and cellular behaviors.

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Section: Hyperspectral-enhanced Dark Field Microscopy (Hedfm)mentioning

confidence: 99%

Hyperspectral-Enhanced Dark Field Microscopy for Single and Collective Nanoparticle Characterization in Biological Environments

Zamora-Pérez

Tsoutsi

et al. 2018

Materials

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“…The spectral imaging technique provides each pixel of SI image a spectral response for each spatial area of a pixel [75]. SI of NPs in cells provides the feasibility of detecting NPs, partial size, surface modification, spatial location, presence of NP agglomeration and wavelength differentiation [76,77]. In this study, we used SI to asses uptake of PVA-AgNP when exposed to CAP resulting to an enhanced PVA-AgNP uptake when cells are exposed to CAP than of NP treatment alone.…”

Section: Discussionmentioning

confidence: 99%

Cold Atmospheric Plasma induces silver nanoparticle uptake, oxidative dissolution and enhanced cytotoxicity in Glioblastoma multiforme cells

Manaloto

Gowen²,

Leśniak³

et al. 2020

Preprint

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Running title: Cold Atmospheric Plasma enhances silver nanoparticle uptake and cytotoxicity.2 Abstract Silver nanoparticles (AgNP) emerged as a promising reagent for cancer therapy with oxidative stress implicated in the toxicity. Meanwhile, studies reported cold atmospheric plasma (CAP) generation of reactive oxygen and nitrogen species has selectivity towards cancer cells. Gold nanoparticles display synergistic cytotoxicity when combined with CAP against cancer cells but there is a paucity of information using AgNP, prompting to investigate the combined effects of CAP using dielectric barrier discharge system (voltage of 75 kV, current is 62.5mA, duty cycle of 7.5kVA and input frequency of 50-60Hz) and 10nm PVA-coated AgNP using U373MG Glioblastoma Multiforme cells. Cytotoxicity in U373MG cells was >100-fold greater when treated with both CAP and PVA-AgNP compared with either therapy alone (IC 50 of 4.30 µg/mL with PVA-AgNP alone compared with 0.07 µg/mL after 25s CAP and 0.01 µg/mL 40s CAP). Combined cytotoxicity was ROS-dependent and was prevented using N-Acetyl Cysteine. A novel darkfield spectral imaging method investigated and quantified AgNP uptake in cells determining significantly enhanced uptake, aggregation and subcellular accumulation following CAP treatment, which was confirmed and quantified using atomic absorption spectroscopy. The results indicate that CAP decreases nanoparticle size, decreases surface charge distribution of AgNP and induces uptake, aggregation and enhanced cytotoxicity in vitro.

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“…Atomic force microscopy, scanning tunneling microscopy, transmission electron microscopy, confocal laser scanning microscopy, fluorescent spectroscopy, spectrophotometry, photothermal microscopy (PTM), X-ray and mass spectrometry are some of the methods used for detection and characterization (Nedosekin et al 2015;Ostrowski et al 2015;Hendrickson et al 2011;Me'rian et al 2012;Théoreta and Wilkinson 2017). Although, dark-field microscopy faces challenges on account of low sensitivity and limited axial resolution, it has found wide use in the detection, identification, and tracking of various NPs with the assistance of multispectral imaging for spectral identification of NPs (Théoreta and Wilkinson 2017;Lee and Tung 2012;Mustafa et al 2013). Confocal fluorescence microscopy remains a powerful biological and widely used technique among the diverse imaging methods, but its drawback is the need for fluorescent NPs.…”

Section: Toxicity Of Nanoparticlesmentioning

confidence: 99%

Toxicity of nanoparticles_ challenges and opportunities

Ramanathan

2019

Appl. Microsc.

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Nanomaterials (NMs) find widespread use in different industries that range from agriculture, food, medicine, pharmaceuticals, and electronics to cosmetics. It is the exceptional properties of these materials at the nanoscale, which make them successful as growth promoters, drug carriers, catalysts, filters and fillers, but a price must be paid via the potential toxity of these materials. The harmful effects of nanoparticles (NPs) to environment, human and animal health needs to be investigated and critically examined, to find appropriate solutions and lower the risks involved in the manufacture and use of these exotic materials. The vast number and complex interaction of NM/NPs with different biological systems implies that there is no universal toxicity mechanism or assessment method. The various challenges need to be overcome and a number of research studies have been conducted during the past decade on different NMs to explore the possible mechanisms of uptake, concentrations/dosage and toxicity levels. This review article examines critically the recent reports in this field to summarize and present opportunities for safer design using case studies from published literature.

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Evaluation of enhanced darkfield microscopy and hyperspectral analysis to analyse the fate of silver nanoparticles in wastewaters

Abstract: Enhanced darkfield microscopy coupled to hyperspectral analysis was evaluated for its capacity to detect Ag nanoparticles in wastewaters and biosolids.

Cited by 27 publications

References 33 publications

Hyperspectral-Enhanced Dark Field Microscopy for Single and Collective Nanoparticle Characterization in Biological Environments

Hyperspectral-Enhanced Dark Field Microscopy for Single and Collective Nanoparticle Characterization in Biological Environments

Cold Atmospheric Plasma induces silver nanoparticle uptake, oxidative dissolution and enhanced cytotoxicity in Glioblastoma multiforme cells

Toxicity of nanoparticles_ challenges and opportunities

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