Evaluation of enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunoelectrotransfer blot (EITB) for immunodiagnosis of hydatid diseases in sheep

Şimşek, Sami; Köroğlu, Ergün

doi:10.1016/j.actatropica.2004.04.006

Cited by 35 publications

(27 citation statements)

References 22 publications

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“…Exclusion of patients who also had liver involvement resulted in a marginal decrease in sensitivity (from 79% to 74%). A decrease in test sensitivity among those with only lung involvement compared with subjects with lung and liver involvement had also been shown in studies using cystic fluid with ELISA (69% to 60%) (19) and Western blotting (94% to 88%) (18). In the bivariate analysis, seropositivity for p176 was associated with having more than one cyst.…”

Section: Discussionmentioning

confidence: 77%

“…This association was evident upon bivariate analysis (89% versus 53% seropositivity) and persisted after adjustment by number of lesions and organ involvement upon multivariate logistic regression analysis. The effect of cyst complications on increasing antibody seropositivity had previ- ously been described in reports of other studies using either ELISA or Western blotting (9,18,19,21). The hydatid cysts isolate their contents from the immune system by developing a very thick collagen layer, leading to minimal or nil antigen release and subsequent minimal or nil antibody responses.…”

Section: Discussionmentioning

confidence: 99%

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Serological Diagnosis of Lung Cystic Hydatid Disease Using the Synthetic p176 Peptide

Santivañez

Arias

Portocarrero

et al. 2012

Clin Vaccine Immunol

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ABSTRACTCystic hydatid disease (CHD) is a worldwide zoonosis caused by the larval stage of the dog tapewormEchinococcus granulosus. Diagnosis is based on imagenological tools (abdominal ultrasound, chest X-rays, or computed tomography [CT] scan). Serological antibody-detecting assays, using diverse native antigens, have been used as a supportive diagnostic tool, but their sensitivities and specificities differ greatly. The use of synthetic peptides as antigens should provide more reliability and allow better assessment and comparison of test formats and case series. The synthetic peptide p176, corresponding to the N-terminal extreme of the subunit of antigen B (AgB8/1), has shown promising performances for diagnosis of CHD. We evaluated the performance of the synthetic peptide p176 for the diagnosis of pulmonary hydatid disease in an enzyme-linked immunosorbent assay (ELISA) format. Sixty-one serum samples from patients with a diagnosis of pulmonary hydatidosis confirmed by surgery and 128 from healthy volunteers were tested. The overall sensitivity and specificity of the p176 ELISA for lung CHD were 78.69% and 96.88%, respectively. On bivariate analysis, positive serum antibody reactions were associated with the presence of complications and with the number of cysts (single/multiple). Only the presence of persistent complications significantly associated with seropositivity on multivariate logistic regression analysis (odds ratio [OR], 9.58; 95% confidence interval [CI], 2.15 to 42.6;P= 0.003). The p176 ELISA performs well for the diagnosis of lung CHD and adds an easily reproducible diagnostic assay to the existing diagnostic tools.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Serological Diagnosis of Lung Cystic Hydatid Disease Using the Synthetic p176 Peptide

Santivañez

Arias

Portocarrero

et al. 2012

Clin Vaccine Immunol

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“…One band with a molecular weight of 116 kDa showed 88% sensitivity and 84% specificity in the immunoblot assay. Sensitivity (60%) was less but specificity was higher (94%) with the HCF-ELISA (Simsek and Koroglu, 2004). Ghorbanpoor et al (2006) were able to detect specific circulating antigens or antibodies in the serum and urine of 13 experimentally infected sheep.…”

Section: Immunodiagnosis Of Cystic Echinococcosis In Intermediate Hosmentioning

confidence: 99%

“…In a later study, Simsek and Koroglu (2004) investigated the antigenic characteristics of hydatid cyst fluid in sheep by SDS-PAGE to evaluate the sensitivity and specificity of HCF-ELISA and immunoblotting for diagnosis of sheep hydatidosis. One band with a molecular weight of 116 kDa showed 88% sensitivity and 84% specificity in the immunoblot assay.…”

Section: Immunodiagnosis Of Cystic Echinococcosis In Intermediate Hosmentioning

confidence: 99%

Recent Advances in the Immunology and Serological Diagnosis of Echinococcosis

Zhang¹,

Li²,

Lin³

et al. 2012

Serological Diagnosis of Certain Human, Animal and Plant Diseases

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“…For instance, antigen B partially purified from HCF of camels or sheep naturally infected with CE as well as its recombinant analogue were used in the enzyme-linked immunosorbent assay (ELI-SA) to test sera from slaughtered camels (Ibrahem et al 2002). Antigenic characteristics of HCF in sheep were investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the efficiency of ELISA and enzyme-linked immunoelectrotransfer blot assay were evaluated for diagnosis of sheep hydatidosis (Simsek and Koroglu 2004).…”

mentioning

confidence: 99%

Serological diagnosis of cystic echinococcosis in cattle

Bulashev¹,

Suranshiev²,

Akibekov³

et al. 2017

FOLIA PARASIT

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An IgM murine monoclonal antibody (MAb) was obtained against the excretory-secretory antigen (ES-Ag) of in vitro reared protoscoleces of Echinococcus granulosus (Batsch, 1786). Western blotting revealed that the MAb recognised a 20.6 kDa protein of this ES-Ag. The MAb was used in sandwich enzyme-linked immunosorbent assay (s-ELISA) for selective sensitisation of the solid phase with the protoscolex-specific protein from its ES-Ag and somatic antigen (S-Ag) to examine serum samples of 108 cows from a cystic echinococcosis (CE) endemic area for specific antibodies and to compare the results with those from necropsies and an indirect ELISA (i-ELISA). The sensitivity of s-ELISA/ES-Ag, s-ELISA/S-Ag and i-ELISA/S-Ag was 48%, 52% and 62%, respectively. The low sensitivity of the ELISA was probably caused by the fact that 13 cows (62%) were infected with sterile cysts (acephalocysts and/or calcified foci) only. A relatively high specificity (80%) of s-ELISA/ES-Ag was observed in cows with fertile cysts. It also detected antibodies in the serum of two cows that had recovered from the disease according to the necropsy. The i-ELISA/S-Ag gave false results in testing sera from a healthy animal and from a cow with tubercular foci. Further analysis will be necessary to define more precisely the value of this study, because the duration of antibody elimination from the bloodstream of recovered cattle remains unknown. The solution of this problem will increase the specificity of the proposed test in monitoring herbivorous animals for CE.

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Evaluation of enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunoelectrotransfer blot (EITB) for immunodiagnosis of hydatid diseases in sheep

Cited by 35 publications

References 22 publications

Serological Diagnosis of Lung Cystic Hydatid Disease Using the Synthetic p176 Peptide

Serological Diagnosis of Lung Cystic Hydatid Disease Using the Synthetic p176 Peptide

Recent Advances in the Immunology and Serological Diagnosis of Echinococcosis

Serological diagnosis of cystic echinococcosis in cattle

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