Evaluation of Epoxy Ether Fixed Bovine Arterial Grafts for Mutagenic Potential

Lohre, Jeffrey M.; Baclig, Lita; Wickham, Emily; Guida, Sarah; Farley, Joanne; Thyagarajan, K; Tu, Roger; Quijano, R C

doi:10.1097/00002480-199304000-00007

Cited by 8 publications

(14 citation statements)

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“…A new ideal graft should be readily available, pliable, easy to use, and most importantly should have excellent long‐term patency. In this study, we have confirmed that a human ureter graft has the following many advantages: a lack of branches and valves, a smooth surface uniformly formed by a basement membrane and a muscle layer resulting from a lack of epithelial cells on the inner surface, a high affinity for the host tissue because it is human tissue, controllability of length and optimal luminal diameter (2–4 mm), host tolerance, tissue compatibility, easy operability, flexibility, elasticity, and resistance to biodegradation ( 9–12).…”

Section: Discussionmentioning

confidence: 52%

“…Additionally, the development of ectopic calcification and the tendency to make tissue less flexible, more hydrophobic, and less adaptable to the host tissue, as well as aneurysm formation and cytotoxicity, have all been implicated in the use of glutaraldehyde as a fixative agent ( 7, 8). To overcome these disadvantages, polyepoxy compounds have been introduced as new crosslinking agents and have been found to be more suitable for this purpose than glutaraldehyde ( 9–12). This experimental study documents the efficacy of the original modified human ureter graft tanned by a new crosslinking agent, polyepoxy compound, for a small caliber arterial prosthesis.…”

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confidence: 99%

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A Modified Human Ureter Graft Tanned by a New Crosslinking Agent Polyepoxy Compound for Small Diameter Arterial Substitutions: An Experimental Preliminary Study

Uematsu

Okada

1998

Artificial Organs

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The effectiveness of human ureter grafts tanned by polyepoxy compound for small diameter arterial substitution was examined in 8 carotid arteries of rabbits, followed by an observation period of either 1 month (n = 6) or 6 months (n = 2). In this model there was no evidence of rejection, aneurysm formation, or infection. The modified human ureter showed excellent patency in all cases as well as excellent function, and the histological evaluation revealed monolayer endothelial-like cells covering all of the surface of the graft as was expected. No intimal hyperplasia was demonstrated at either anastomotic site. This study suggests that our newly designed polyepoxy compound fixed human ureter could serve as a satisfactory blood conduit with good long-term patency for substitution.

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Section: Discussionmentioning

confidence: 52%

mentioning

confidence: 99%

A Modified Human Ureter Graft Tanned by a New Crosslinking Agent Polyepoxy Compound for Small Diameter Arterial Substitutions: An Experimental Preliminary Study

Uematsu

Okada

1998

Artificial Organs

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“…This result is usually achieved through cross‐linking agents . Commonly used chemical cross‐linkers include formaldehyde, glutaraldehyde, polyepoxy compounds, carbodiimides, and acyl azide . However, these synthetic cross‐linking reagents are relatively high cytotoxic, impairing the biocompatibility of the cross‐linked material …”

Section: Introductionmentioning

confidence: 99%

Genipin diffusion and reaction into a gelatin matrix for tissue engineering applications

Montemurro

Maria

Orsi

et al. 2015

J. Biomed. Mater. Res.

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Genipin is a natural low-toxic cross-linker for molecules with primary amino groups, and its use with collagen and gelatin has shown a great potential in tissue engineering applications. The fabrication of scaffolds with a well-organized micro and macro topology using additive manufacturing systems requires an accurate control of working parameters, such as reaction rate, gelling time, and diffusion constant. A polymeric system of 5% w/v gelatin in PBS with 2 mg/mL collagen solutions in a 1:1 weight ratio was used as template to perform measurements varying genipin concentration in a range of 0.1-1.5% w/w with respect to gelatin. In the first part of this work, the reaction rate of the polymeric system was estimated using a new colorimetric analysis of the reaction. Then its workability time, closely related to the gelling time, was evaluated thanks to rheological analysis: finally, the quantification of static and dynamic diffusion constants of genipin across nonreacting and reacting membranes, made respectively by agarose and gelatin, was performed. It was shown that the colorimetric analysis is a good indicator of the reaction progress. The gelling time depends on the genipin concentration, but a workability window of 40 min guaranteed up to 0.5% w/w genipin. The dynamic diffusion constant of genipin in the proposed polymeric system is in the order of magnitude of 10 . The obtained results indicated the possibility to use the genipin, gelatin, and collagen, in the proposed concentrations, to build well-defined hydrogel scaffolds with both extrusion-based and 3D ink-jet system. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 473-480, 2017.

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“…It was shown in vitro and in vivo that the epoxy-fixed tissue is superior in pliability and inhibition of calcification to its glutaraldehyde-fixed counterpart [3,8,9]. Furthermore, the epoxy compound solution is less cytotoxic than glutaraldehyde solution [10][11][12] . Lohre et al [10,11 ] reported that the maximum concentrations of glycerol polyglycidyl ether and ethylene glycol diglycidyl ether solutions found to demonstrate noncytotoxic were 55 and 60 ppm by the medium eluate method and 150 and 200 ppm by the agar overlay procedure, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the epoxy compound solution is less cytotoxic than glutaraldehyde solution [10][11][12] . Lohre et al [10,11 ] reported that the maximum concentrations of glycerol polyglycidyl ether and ethylene glycol diglycidyl ether solutions found to demonstrate noncytotoxic were 55 and 60 ppm by the medium eluate method and 150 and 200 ppm by the agar overlay procedure, respectively. Similar findings were also reported by Nishi et al [12] in an in vitro study to investigate the toxicity of various epoxy compounds used for fixing biological tissues.…”

Section: Introductionmentioning

confidence: 99%

In vitro study of enzymatic degradation of biological tissues fixed by glutaraldehyde or epoxy compound

Sung

Chen

Tsai

et al. 1997

Journal of Biomaterials Science, Polymer Edition

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The study, using bacterial collagenase, was to investigate the changes in characteristics of a collagen-rich tissue, porcine pericardium, fixed by glutaraldehyde or epoxy compound (ethylene glycol diglycidyl ether) during the course of degradation. Fresh porcine pericardium was used as a control. During degradation, the heat released by the reaction of collagenase with a test sample was monitored by a highly sensitive microcalorimeter. Also, the degree of degradation of each test sample was determined by measuring its increment in free amino group content and changes in denaturation temperature and tensile strength. Microcalorimetric analysis of collagenase degradation of fresh, epoxy-fixed, and glutaraldehyde-fixed tissues revealed that the heat released during degradation correlates well with the degree of tissue degraded. The cleaving of peptide bonds in biological tissue by collagenase degradation may increase its free amino group content and reduce its denaturation temperature and tensile strength. It was noted that the fresh tissue cannot resist bacterial collagenase degradation, while the glutaraldehyde-fixed tissue had a relatively better resistance to degradation than its epoxy-fixed counterpart.

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Evaluation of Epoxy Ether Fixed Bovine Arterial Grafts for Mutagenic Potential

Cited by 8 publications

References 0 publications

A Modified Human Ureter Graft Tanned by a New Crosslinking Agent Polyepoxy Compound for Small Diameter Arterial Substitutions: An Experimental Preliminary Study

A Modified Human Ureter Graft Tanned by a New Crosslinking Agent Polyepoxy Compound for Small Diameter Arterial Substitutions: An Experimental Preliminary Study

Genipin diffusion and reaction into a gelatin matrix for tissue engineering applications

In vitro study of enzymatic degradation of biological tissues fixed by glutaraldehyde or epoxy compound

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