Evaluation of F2 and F3 plants introgressed with QTLs for clubroot resistance in cabbage developed by using SCAR markers

Nomura, Kazunari; Minegishi, Yoshiyuki; Kimizuka-Takagi, Chiaki; Fujioka, Takashi; Moriguchi, Katsumi; Shishido, Rieko; Ikehashi, Hiroshi

doi:10.1111/j.1439-0523.2005.01105.x

Cited by 41 publications

(24 citation statements)

References 13 publications

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“…Mapping studies using molecular markers also revealed polygenic nature of the CR trait in B. oleracea (Landry et al 1992, Figdore et al 1993, Grandclément and Thomas 1996, Voorrips et al 1997, Moriguchi et al 1999, Rocherieux et al 2004, Nomura et al 2005. However, these studies showed the presence of a CR gene having large effect in this species.…”

Section: B Oleraceamentioning

confidence: 99%

Genetic Analysis of Clubroot Resistance in Brassica Crops

Hirai

2006

Breed. Sci.

109

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Clubroot disease is caused by an obligate parasite, Plasmodiophora brassicae, and is one of the most serious diseases of Brassica crops worldwide. The pathogen is a eukaryote, and is a member of Plasmodiophorales. Recent phylogenic studies did not reveal its close relationship to fungi, but suggest a classification in Protozoa or Protoctista. The infection is thought to occur through two phases. The pathogen induces massive clubs on root, and prevents host growth. A number of clubroot resistance (CR) Chinese cabbage (Brassica rapa) cultivars have been bred using European turnips as sources of CR genes. Field populations of the pathogen have a wide variation of virulence, which causes the infection of CR Chinese cabbage cultivars. The CR gene of B. rapa is extensively studied with the aid of molecular markers. At least four independent loci are identified and mapped. They are all derived from European turnips. Crr1, and Crr2 are derived from Siloga and mapped in linkage groups R8 and R6, respectively. Crr3 from Milan White, and CRb from Gelria R are both mapped in R3. The molecular markers linked to Crr1, Crr2 and CRb showed homology to the central part of the long arm of chromosome 4 of Arabidopsis thaliana. These findings suggest that these CR genes are derived from the same region of the ancestral genome. In contrast, Crr3 is thought to have different origin, since its linkage markers showed homology to chromosome 3 of A. thaliana. Although another CR gene CRa is reported, the relationship between above mentioned four CR loci and CRa is not yet clarified. Genetic studies on CR genes in B. oleracea revealed a polygenic nature of the trait in this species and also suggest the presence of one CR gene having large effect. However, correspondence among the CR genes reported in these studies is not clarified because of the lack of information on base sequence of the linkage markers in this species. Strategies for breeding of more resistant Chinese cabbage cultivars to overcome the variation on virulence of field populations of the pathogen are presented.

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Section: B Oleraceamentioning

confidence: 99%

Genetic Analysis of Clubroot Resistance in Brassica Crops

Hirai

2006

Breed. Sci.

109

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“…Moreover, synthetic amphidiploid Raphanobrassica, which was developed by the hybridization of Raphanus and Brassica, also have high levels of clubroot resistance (McNaughton 1973, Yoshikawa 1981. However, the CR gene(s) of radish has not been investigated in comparison with that of Brassica crops such as B. rapa (2n = 20, AA), B. oleracea (2n = 18, CC), and B. napus (Kuginuki et al 1997, Moriguchi et al 1999, Manzanares-Dauleux et al 2000, Suwabe et al 2003, 2006, Hirai et al 2004, Nomura et al 2005. Although QTL analysis was conducted previously by Kamei et al (2007), the clubroot resistance of radish has not been investigated at the chromosomal level.…”

Section: Introductionmentioning

confidence: 99%

Identification and evaluation of clubroot resistance of radish chromosome using a Brassica napus-Raphanus sativus monosomic addition line

et al. 2009

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Clubroot resistance (CR) against the Ano-01 isolate of the clubroot pathogen Plasmodiophora brassicae for individual radish chromosomes was evaluated using 9 types (a-i) of Brassica napus-Raphanus sativus monosomic addition lines (MALs) produced from the progenies of the hybridization of B. napus (2n = 38, AACC) and Raphanobrassica 'Rb-63' (2n = 36, RRCC). Among the 9 types of MAL, the c-type showed high resistance, although other types and reverted plants with somatic chromosomes like B. napus (2n = 38) were susceptible. This result suggested that a major CR gene against the Ano-01 isolate may be located on the radish c-chromosome. However, it was also assumed that there might be other CR gene(s) in radish because many c-type MAL plants showed slight disease symptoms.

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“…In cabbage breeding programs for disease resistance, the identification of resistance sources is performed in parallel with the recovery of marketing type and the elimination of undesirable traits from the resistance source. This is particularly difficult when inter-specific crosses are made with resistance sources (Nomura et al 2005), or during the incorporation of the resistance trait into the desired morphotypes of B. oleracea (Bagget and Kean 1985). However, significant variability in resistance to clubroot was found among different cultivars of B. oleracea (Diederichsen et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Chinese cabbage (Brassica rapa ssp. pekinensis) – a valuable source of resistance to clubroot (Plasmodiophora brassicae)

et al. 2016

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Clubroot, caused by the protozoan parasite Plasmodiophora brassicae Woronin, is one of the most damaging diseases of Brassica napus worldwide. Resistant plant material is valuable for cultivation in all areas of high incidence of the disease and intensive growth of oilseed rape. We have evaluated clubroot resistance, plant morphology and seed quality in 15 lines of an F 4 generation and selected six lines of F 5 generation of interspecific hybrids obtained from a cross between a male sterile line of B. napus 'MS8', selected from resynthesized oilseed rape (B. rapa ssp. chinensis × B. oleracea var. gemmifera) and an ecotype of B. rapa ssp. pekinensis. Clubroot resistance was evaluated using a bioassay with P 1 -P 5 pathotypes of P. brassicae (according to the classification of Somé et al. 1996). The resistance to the pathotype P 1 was successfully fixed in the F 5 generation, and improved in some lines in respect to the pathotypes P 2 -P 4 . The resistance to P 1 and the other tested pathotypes was not linked. Characterization of plant material included recent techniques of FISH and BAC-FISH with a special focus on the analysis of ribosomal DNA (rDNA) of selected individuals. Two hybrid lines combined high levels of resistance with appropriate plant morphology, good seed quality traits and a stable chromosome number and arrangement. Recent techniques of 'chromosome painting' provided good insight into chromosome organization in the hybrids obtained, and offered opportunities of further improvement of the breeding process.

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Evaluation of F2 and F3 plants introgressed with QTLs for clubroot resistance in cabbage developed by using SCAR markers

Cited by 41 publications

References 13 publications

Genetic Analysis of Clubroot Resistance in Brassica Crops

Genetic Analysis of Clubroot Resistance in Brassica Crops

Identification and evaluation of clubroot resistance of radish chromosome using a Brassica napus-Raphanus sativus monosomic addition line

Chinese cabbage (Brassica rapa ssp. pekinensis) – a valuable source of resistance to clubroot (Plasmodiophora brassicae)

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