Evaluation of fish skin as a biological dressing for metacarpal wounds in donkeys

Abstract

Background The use of biological dressings has recently emerged in the management of burns and wounds. The aim of the present study was to evaluate the Nile tilapia skin as a biological dressing for full-thickness cutaneous metacarpal wounds in donkeys. The study was conducted on nine clinically healthy donkeys (n = 9). Here, fish skin dressings were obtained from fresh Nile tilapia (Oreochromis niloticus and sterilized by immersion in silver nanoparticles (AgNPs) solution for 5 min, with no change in collagen content. Bilateral, circular full-thickness excisional skin wounds (2 cm in diameter) were created on the dorsal aspect of the mid-metacarpals of each donkey. Wounds on the right metacarpals (treated wounds, n = 9) were dressed with sterile fish skins, while wounds on the left metacarpals (control wounds, n = 9) were dressed with sterile non-adherent dressing pads without any topical applications. Wound dressings were changed weekly. Wounds were evaluated microbiologically, grossly, and histologically on days 7, 14, and 21 post-wound inductions. Results Fish skin-dressed wounds showed a significant (P < 0.0001) reduction in microbial counts (Total viable bacterial count, Staphylococcal count, and Coliform count), a significant (P < 0.0001) decrease in the wound size, and a significant reduction (P < 0.0001) in the epithelial gap compared to the untreated wounds. No frequent dressing changes were needed. Conclusions Fish skin dressing accelerated the wound healing process and efficiently inhibited the local microbial activity and exuberant granulation tissue formation suggesting its reliable and promising application for metacarpal wounds of donkeys.

Evaluation of the bactericidal effect of silver nanoparticles against Methicillin Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) strains isolated from mastitic milk of small ruminants and their surrounding environment in Aswan

“…Therefore, AgNPs have been commonly used as antimicrobial agent against drug resistant bacterial strains (Kvítek et al, 2008;Jones and Hoek, 2010;Lara et al, 2010;Ansari et al, 2011). AgNPs can also be applied in cases of burn, traumatic wound dressings, diabetic ulcers, coating of catheters, dental works, medical devices and water filtration (Jain and Pradeep, 2005;Silver et al, 2006;Gong et al, 2007;Kim et al, 2007;Thomas et al, 2007;Rai et al, 2009;Martinez-Gutierrez et al, 2010;Dosoky et al, 2015;Ibrahim et al, 2020). In the present study, the bactericidal effect of silver nanoparticles against MRSA and MSSA strains isolated from mastitic milk of small ruminants and their surrounding environment in Aswan was investigated.…”

Medicina e bem-estar de animais silvestres e exóticos, vol. 1

“…Nesse trabalho o protocolo de preparação do curativo biológico seguiu etapas das metodologias citadas por outros autores como o processo de limpeza, recorte e esterilização (MIRANDA, 2018;IBRAHIM et al, 2020). Entretanto, baseando-se no trabalho descrito por Lima-Junior et al (2017), optou-se pela aplicação das etapas de preparo da pele, devido a praticidade, eficácia e por utilizar materiais de baixo custo.…”

“…Recentemente o enxerto de pele tem sido uma das técnicas reconstrutivas utilizadas por cirurgiões plásticos como alternativa biológica para auxiliar na cicatrização de feridas em pacientes humanos e em animais (SHIMIZU-HAYASHI e KISHI, 2012;IBRAHIM et al, 2020).…”

The granulation (t)issue: A narrative and scoping review of basic and clinical research of the equine distal limb exuberant wound healing disorder

“…Application of equine amnion resulted in significantly less EGT J o u r n a l P r e -p r o o f formation than a non-occlusive dressing (Bigbie et al, 1991), although this was unconfirmed in other studies (Howard et al, 1993). A recent study investigating Nile tilapia skin on induced mid-metacarpal wounds of donkeys found the dressing resulted in no EGT formation, whereas control wounds treated with non-adherent pads showed signs of EGT (Ibrahim et al, 2020).…”