Evaluation of fungicides for the management of Botryosphaeria dieback diseases of grapevines

Amponsah, N.T.; Jones, E. Eirian; Ridgway, Hayley J.; Jaspers, Marlene V.

doi:10.1002/ps.2309

Cited by 62 publications

(35 citation statements)

References 15 publications

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“…seriata, N. australe and N. parvum (Bester and Fourie, 2005;Savocchia et al, 2005;Bester et al, 2007;Auger et al, 2010;Amponsah et al, 2012). Additionally, our results agree with a recently published report (Pitt et al, 2012) indicating that tebuconazole and myclobutanil are very effective at controlling the mycelial growth of D. seriata and N. parvum in vitro.…”

Section: Discussionsupporting

confidence: 91%

Evaluation of DMI fungicides against species of Diplodia and Neofusicoccum associated with Botryosphaeria canker of grapevine

et al. 2013

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C. Torres, B.A. Latorre, P. Undurraga, and X. Besoain. 2013. Evaluation of DMI fungicides against species of Diplodia and Neofusicoccum associated with Botryosphaeria canker of grapevine. Cien. Inv. Agr. 40(1):131-138. The Botryosphaeriaceae species have been widely studied and are considered a serious problem to the production of grapes worldwide. To aid in the development of control strategies for these fungi, in vitro experiments were conducted to evaluate the effectiveness of demethylation inhibiting (DMI) fungicides against Diplodia seriata, D. mutila, Neofusicoccum australe and N. parvum isolated from grapevine (Vitis vinifera). Fungicidal effectiveness was evaluated over a range of increasing concentrations using measurements of both the inhibition of conidial germination and the effects on mycelial growth. The effective concentrations (EC) necessary to inhibit conidial germination and mycelial growth by 50% (EC 50 ) and 90% (EC 90 ) were estimated by linear regression analysis between x = log 10 of the fungicidal concentration and y = the Probit value of the inhibition percentage. The results demonstrate that tebuconazole, myclobutanil, prochloraz and prochloraz plus epoxiconazole exhibit considerable in vitro activity against D. mutila, D. seriata, N. australe and N. parvum. These DMI compounds were able to arrest conidial germination and inhibit mycelial growth. The EC 50 values obtained for conidial germination were generally higher than the EC 50 values obtained for mycelial growth, suggesting that mycelia are more sensitive to the DMI compounds than are conidia. However, the effectiveness of these DMI compounds in the vineyard must be studied before any final conclusions can be reached.

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Section: Discussionsupporting

confidence: 91%

Evaluation of DMI fungicides against species of Diplodia and Neofusicoccum associated with Botryosphaeria canker of grapevine

et al. 2013

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“…Many different studies have been carried out to monitor pruning wound protection with chemical antifungals such as benomyl, carbendazim, fosetyl, tebuconazole, or thiophanate-methyl, among others (7,20,23,25,26,45), to cite only the latest. Some of these chemical fungicides have proven to be effective at different rates in the protection of pruning wounds.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, when Rolshausen et al (7) evaluated the efficacy of different fungicides in controlling both Petri disease pathogens and infections from several Botryosphaeriaceae species, they found that thiophanate-methyl was most effective. More recently, Amponsah et al (25) reported that 9 out of the 16 fungicides tested were effective in inhibiting the growth of several Botryosphaeriaceae species, reducing mycelial growth and/or conidial germination. Assays on potted and field grapevines showed various degrees of protection; flusilazole and carbendazim were the most effective.…”

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confidence: 99%

Effectiveness of Natural Antifungal Compounds in Controlling Infection by Grapevine Trunk Disease Pathogens through Pruning Wounds

Cobos

Mateos

Álvarez-Pérez

et al. 2015

Appl Environ Microbiol

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Grapevine trunk fungal pathogens, such as Diplodia seriata and Phaeomoniella chlamydospora, can infect plants through pruning wounds. They cause grapevine trunk diseases and are involved in grapevine decline. Accordingly, the protection of pruning wounds is crucial for the management of grapevine trunk diseases. The efficacy of different natural antifungals in inhibiting the growth of several fungi causing grapevine trunk diseases was evaluated in vitro. The fungi showing greater in vitro efficacy were tested on autoclaved grape wood assays against D. seriata and P. chlamydospora. Based on results from these assays, chitosan oligosaccharide, vanillin, and garlic extract were selected for further evaluation on pruning wounds inoculated with D. seriata and P. chlamydospora in field trials. A significant decrease in plant mortality was observed after 2 years of growth in the plants treated with the different natural antifungals compared to the mortality rate observed in infected plants that were not treated with antifungals. Also, the infection rate for the inoculated pathogens was significantly reduced in plants treated with the selected natural antifungals. Therefore, natural antifungals represent a promising alternative for disease control and could provide significant economic benefits for the grape-growing industry. Grapevine trunk diseases are a major threat to the wine and grape industry. They have been reported in most grapevineproducing regions worldwide, causing serious economic losses due to a significant reduction of both yields and the quality of the grapes (1-3). The diseases include Botryosphaeria dieback, esca, Eutypa dieback, Petri disease, black foot, and Phomopsis dieback as the most relevant pathologies.Planting material produced in nurseries is frequently infected with fungal pathogens, especially those involved in Petri and black foot diseases (4, 5). In mature vineyards, pathogens such as Ilyonectria sp. mainly penetrate through the root system (6). However, it is widely accepted that for many pathogens, the annually produced pruning wounds are the main route of entry into young and adult plants (7-9). Consequently, numerous studies have been conducted to assess the susceptibility of pruning wounds to fungal pathogens causing trunk diseases. Susceptibility to Botryosphaeriaceae species has been studied by several authors (8-10). Eutypa lata infection has been extensively analyzed by authors like Munkvold and Marois (11) (8) also studied susceptibility to Phomopsis viticola infection. All of these studies support the hypothesis that pruning wounds are the main route of entry of these pathogens in the plant, and, therefore, the development of measures to protect pruning wounds would be an essential tool in the management of vineyards with better sanitation. Accordingly, numerous tests treating pruning wounds with chemical fungicides and/or biological control agents (BCA) have been carried out. Munkvold and Marois (16) checked the efficacy of natural epiphytes and colonizers of pruning wounds fo...

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“…Therefore, based on the presented results OA medium could be recommended as a suitable for radial growth assays for D. applanata, despite the fact that PDA medium is one of the most commonly used culture media for in vitro fungicide sensitivity evaluation. [21][22][23][24] The results also showed that most of the D. applanata isolates collected at five different locations in western Serbia were sensitive to the fungicides tested. However, the EC 50 values differed depending on both fungicides and isolates.…”

Section: Discussionmentioning

confidence: 98%

Toxicity of copper hydroxide, dithianon, fluazinam, tebuconazole and pyraclostrobin toDidymella applanataisolates from Serbia

Mirković

Tanović

Hrustić

et al. 2015

Journal of Environmental Science and Health, Part B

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A study of the in vitro sensitivity of 10 isolates of Didymella applanata to copper hydroxide, dithianon, fluazinam, tebuconazole and pyraclostrobin, was conducted. The isolates were derived from diseased raspberry canes sampled during 2013 at five localities in western part of Serbia, known as the main raspberry growing region of the country. Prior to sensitivity testing experimental conditions for radial growth assay were optimized. The results showed that the temperature of 22 °C, oatmeal agar medium and 12/12 hrs light/ darkness light regimen provided the best conditions for sensitivity tests. Most of D. applanata isolates were sensitive to the tested fungicides. The narrowest range of EC50 values was recorded for tebuconazole (1.42-2.66 mg L(-1)). The widest range of EC50 values was obtained for pyraclostrobin, ranging from 0.17 mg L(-1) to 55.33 mg L(-1). The EC50 values for the studied isolates were 39.48-51.19 mg L(-1) for copper hydroxide, 12.12-18.73 mg L(-1) for dithianon and 5.72-42.56 mg L(-1) for fluazinam. According to resistance factor values, all D. applanata isolates were sensitive to copper hydroxide, dithianon and tebuconazole. Among tested isolates, six were highly resistant to pyraclostrobin (RFs in the range of 207.1-325.5) and two moderately resistant to fluazinam (RFs were 3 and 7.4), respectively.

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Evaluation of fungicides for the management of Botryosphaeria dieback diseases of grapevines

Abstract: This research has demonstrated that fungicides applied after winter pruning can protect vines from infection by conidia of three botryosphaeriaceous species.

Cited by 62 publications

References 15 publications

Evaluation of DMI fungicides against species of Diplodia and Neofusicoccum associated with Botryosphaeria canker of grapevine

Evaluation of DMI fungicides against species of Diplodia and Neofusicoccum associated with Botryosphaeria canker of grapevine

Effectiveness of Natural Antifungal Compounds in Controlling Infection by Grapevine Trunk Disease Pathogens through Pruning Wounds

Toxicity of copper hydroxide, dithianon, fluazinam, tebuconazole and pyraclostrobin toDidymella applanataisolates from Serbia

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