Evaluation of Genetic Diversity among Persimmon Cultivars (Diospyros kaki Thunb.) Using Microsatellite Markers

Abstract

The genetic diversity among 48 persimmon (Diospyros kaki Thunb.) accessions, indigenous in Korea and introduced from Japan and China, was evaluated by using simple sequence repeat (SSR) markers. From 20 SSR primer sets, a total of 114 polymorphic markers were detected among 12 pollination-constant non-astringent (PCNA), 13 pollination-variant non-astringent (PVNA), 15 pollination-variant astringent (PVA), and 8 pollination-constant astringent (PCA) cultivars. Analysis of pair-wise genetic similarity coefficient (Nei-Li) and unweighted pair-group method with arithmetic averaging (UPGMA) clustering revealed two main clusters and four subclusters for cluster I. The subclustering pattern was in accordance with the classification of persimmon cultivars based on the nature of astringency loss. Phenetic relationships among the subclusters showed a closer relatedness of the PCNA group with the PVNA group, and the PVA with the PCA group. Genetic similarity co-efficiency was 0.499 on average and the highest (0.954) similarity was observed between 'Cheongdo-Bansi' and 'Haman-Bansi'. The similarity was lowest (0.192) between 'Damopan'and 'Atago'. Identification of each cultivar with the execption of 'Cheongdo-Bansi' and 'Gyeongsan-Bansi' was possible based on the SSR fingerprints, suggesting that these SSR markers are a useful tool for protecting intellectual property on newly developed cultivars.

Development of EST-SSR Markers and Analysis of Genetic Diversity Using Persimmon (Diospyros kaki Thunb) Cultivars Collecting from Domestic

“…전세계에 분포하는 감나무 속(Diospyros) 식물은 대략 190여종이다. 그 중 과수로 이용되고 있는 것은 감(Diospyros kaki Thunb), 고욤(Diospyros louts L), 미국감(Diospyros virginiana L), 유시(Diospyros oleifera Cheng) 등 4종 이다 (Hwang et al, 2010;Lim et al, 2008 (Cho et al, 2007). 지금 까지 식물 종 구분을 위하여 사용된 DNA 마커에는 RFLP(restriction fragment length polymorphism), RAPD (random amplified polymorphic DNAs), AFLP (amplified fragment length polymorphism), SSR(simple sequence repeat) 등이 있다 (Powell et al, 1996).…”

“…최근에는 다양한 조직에서 발현되는 유전자에 대한 대량의 정보를 알아내고 실제 발현되는 유전자를 근거로 유전자의 기능을 이해할 수 있을 뿐만 아니라, 염색체 위치를 밝히는데 유용한 EST (expressed sequence tags)-SSR 분석법이 많이 활용되 고 있다 (Kim et al, 2010;Oh et al, 2010;Qureshi et al, 2004). 최근 들어서는 감을 재료로 DNA 마커를 이용 한 유전적 분석은 RAPD (Je et al, 2009;Yamagishi et al, 2005;Yýldýz et al, 2007), AFLP (Cho et al, 2007), RFLP(Hu et al, 2008), SSR (Hwang et al, 2010;Guo et al, 2006;Naval et al, 2010;Zhang et al, 2009) 을 이용해 분석한 바 있지만 AFLP분석과 RAPD분석은 실 험의 어려움과 재현성 여부에 문제가 있으며, 연구에 사용 된 재료가 주로 일본이나 중국품종이어서 우리 실정에 맞 지 않고 PCR에 사용된 primer도 외국에서 개발한 primer 를 사용해 우리나라 품종을 분석한 바 있다 (Hwang et al, 2010 Fig. 4.…”

Development of Sequence Characterized Amplified Region Markers for Cultivar Identification in Persimmon

“…'부유(Fuyu)'와 불완전단감인 '서촌조생(Nishimurawase)' 등 과 같은 일본품종이 대부분이다 (Hwang et al, 2010). (Maki et al, 2001;Nakamura and Kobayashi, 1994), randomly amplified polymorphic DNA(RAPD) (Badenes et al, 2003;Luo et al, 1995;Yamagishi et al, 2005), amplified fragment length polymorphism(AFLP) (Cho et al, 2007;Yonemori et al, 2008), sequence-related amplified polymorphism(SRAP) (Guo and Luo, 2006), simple sequence repeats(SSR) (Hwang et al, 2010;Naval et al, 2010;Park et al, 2012) 등과 같은 다양한 DNA 마커들이 …”

“…이 중 RAPD는 적은 양의 DNA를 이용하여 신 속하게 저렴한 비용으로 유전적 다형성을 분석할 수 있다는 장점이 있어 사과 (Goulão et al, 2001), 배 (Lin et al, 2011), 복숭아 (Yu et al, 2012) (Cho et al, 2010(Cho et al, , 2012Vidal et al, 2000). 한 그룹으로 보고되었는데 (Hwang et al, 2010;Je et al, Table 4. M, 100 bp plus DNA ladder.…”

Analysis of microbial communities in local cultivars of astringent persimmon (Diospyros kaki) fruits grown in Gyeongnam Province of Korea

“…Astringent persimmons contain water-soluble tannin components has a bitter taste and tannic acid which has been shown to have anti-aging, anticancer and herbivory defense effects (Park ., 2004;Parfitt ., 2015;Kim ., 2011a Schools, 2004;Heo ., 2001;Chinese Pharmacopoeia Commission, 2010). Although genetic diversity and pharmacological studies of astringent persimmons have recently been reported from native provinces (Hwang ., 2010;Choi ., 2016), few studies have attempted to analyze the diversity of microorganisms on astringent persimmons. Thus, this study characterized and identified the microorganisms found on the native cultivars of astringent persimmon using pyrosequencing-a meta-genomic analysis technique.…”