Evaluation of genetic diversity in self-incompatible broccoli DH lines assessed by SRAP markers

Abstract

In this article, we investigated self-compatibility index (SCI) in broccoli double haploid (DH) lines, and the relationship and genetic diversity of 15 self-incompatible (SI) broccoli DH lines were analyzed by sequence related amplified polymorphism (SRAP). 11 primer combinations selected from 88 primer pairs revealed a total number of 129 unambiguous bands, 61 of which were polymorphic with a polymorphism frequency of 47.3%. Analyzed by NTSYS software, the genetic similarity coefficient of the 15 broccoli resources ranged from 0.76 to 0.98. Based on the coefficient value of 0.79, these broccoli DH lines were clustered into three multiple-member groups by unweighted pair group method with arithmetic mean (UPGMA) analysis, which provided molecular reference for parent selection in broccoli breeding.

Development of GBTS and KASP Panels for Genetic Diversity, Population Structure, and Fingerprinting of a Large Collection of Broccoli (Brassica oleracea L. var. italica) in China

“…For genetic improvement and conservation of crop species, it is vital to study the extent of genetic diversity and population structure prevailing in the species. Previously, only few studies were carried out to decipher the genetic diversity of broccoli using molecular markers (Tonguç and Griffiths, 2004;Lu et al, 2009;Huifang et al, 2011;Ciancaleoni et al, 2014;Stansell et al, 2018). However, a major concern is that, in most of the studies, there was poor representation of samples, or a limited number of samples was used raising the apprehension that the actual diversity present in broccoli has been underestimated.…”

Genetic Diversity of Moroccan Orobanche crenata Populations Revealed by Sequence-Related Amplified Polymorphism Markers

“…The SRAP technique was very useful for the assessment of genetic diversity because it presents many merits over the other marker systems (Aneja, Yadav, Chawla, & Yadav, 2012). It was used in assessing genetic diversity in many species , including lentil (Rana, Singh, & Bhat, 2009), pea (Esposito, Martin, Cravero, & Cointry, 2007), Alfalfa (Al-Faifi et al, 2013;Castonguay, Cloutier, Bertrand, Michaud, & Laberge, 2010), mungbean (Aneja, 2010), quigke (Yang, Liu, Yang, & Feng, 2015), broccoli (Yu, Zhao, Sheng, Wang, & Gu, 2013), etc.…”