Evaluation of genetic fidelity of in vitro-propagated blackberry plants using RAPD and SRAP molecular markers

Borsai, Orsolya; Hârța, Monica; Szabo, Katalin; Kelemen, Cristina-Daniela; Andrecan, Flavia Andreea; Codrea, Maria-Maruni; Clapa, Doina

doi:10.17221/20/2019-hortsci

Cited by 27 publications

(19 citation statements)

References 27 publications

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“…In order to assess the genetic variability of berry plants, the RAPD and ISSR markers are most frequently used. In several research papers, the use of AFLP, SSR, and SRAP markers was also reported [17,19,21,25,[61][62][63]65,[70][71][72]. AFLP analysis proved to be more reliable and sensitive than other methods not only in our research, but also in the studies of Lanham and Brennan [21] and Costa and co-workers [31].…”

Section: Discussionsupporting

confidence: 72%

“…In plants derived from four-year pineapple in vitro cultures, the analysis, with the use of RAPD markers, showed as much as 95.9% variability [64]. On the other hand, Borsai and co-workers [65], using sequence-related amplified polymorphism (SRAP) and RAPD markers, did not detect any polymorphism in plants of blackberry cv. Loch Ness and Chester Thornless propagated for both 3 and 11 subcultures.…”

Section: Discussionmentioning

confidence: 99%

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Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (Ribes grossularia L.)

2020

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Gooseberry (Ribes grossularia L.) is a small fruit crop producing valuable fruits, which is constantly gaining importance. In vitro propagation of this species can significantly support the production of virus-free planting material and accelerate the introduction of new cultivars to the market. The aim of presented study was to assess field performance and genetic stability of micropropagated plants (MPs) of four gooseberry cultivars, “Captivator”, “Hinnonmaki Rot”, “Invicta”, and “Resika”. The growth vigor and yield of MPs and plants propagated by standard methods from softwood cuttings (ST) were evaluated in a field experiment. Microscopic observations of the number and length of the stomata of MP and ST plants were carried out. Two DNA-based techniques, amplified fragment length polymorphism (AFLP) and inter simple sequence repeat (ISSR), were used to assess genetic stability of MP plants. For analysis of genetic stability of ST plants, the ISSR technique was applied. For three cultivars, Captivator, Hinnonmaki Rot, and Invicta, the plants’ growth vigor and fruit yield were greater in MP plants than in ST plants. In the case of Resika, most of these parameters were higher in ST plants. Microscopic observations of the stomata indicated a lack of differences in the length between MP and ST plants, while the stomata frequency on leaves of MP plants was higher than that of ST plants. The genetic variability of MP plants, assessed by AFLP, ranged from 0.35% for Hinnonmaki Rot to 2.12% for Resika. The results of ISSR analysis of MP plants showed variability from 0% in the case of Hinnonmaki Rot and Resika to 4% and 8.69% for Captivator and Invicta, respectively. No polymorphism was detected among ST plants of all analyzed gooseberry cultivars.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (Ribes grossularia L.)

2020

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“…Epigenetic changes are often temporary, and plants may revert to the normal phenotype relatively easily (Jain 2001;Smulders & de Klerk 2011). Molecular markers become the most desirable tool for establishing genetic uniformity of in vitro derived plantlets of different plant species, including berry plants such as lowbush blueberry, strawberry and blackberry (Debnath 2011(Debnath , 2013Borsai et al 2020). To date, there is no genetic stability assessment system for micropropagated plants of blue honeysuckle.…”

Section: ____________________________________________________________mentioning

confidence: 99%

Ex vitro Rooting, Acclimatization and Genetic Stability of Lonicera caerulea var. kamtschatica

Wojtania

Markiewicz

Góraj-Koniarska

2020

Journal of Horticultural Research

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Ex vitro rooting and acclimatization of two cultivars ‘Wojtek’ and ‘Zojka’ of blue honeysuckle (Lonicera caerulea var. kamtschatica Sevast.) were studied. To the ex vitro conditions were transferred rooted and unrooted shoots. The post-effect of auxin type and concentration as well as microcutting and soil substrate types were tested. The genetic stability of the plantlets in relation to the mother plants by using amplified fragment length polymorphism (AFLP) and inter simple sequence repeat (ISSR) markers has been also determined. It has been found that in vitro rooted cuttings of both cultivars showed a higher survival rate (max. 88%) and better growth and development when they were rooted on a medium containing a low auxin level (1.0 mg·dm−3). The results of the second experiment showed successful ex vitro rooting of blue honeysuckle shoots without auxin treatment. Higher ex vitro rooting and survival rate in the greenhouse have been observed for ‘Wojtek’ (max. 96%) than ‘Zojka’ (max. 88%). Better growth and development of shoots and roots were observed on peat alone or a mixture of peat and perlite as compared to a mixture of peat and sand. The micropropagated plantlets appeared similar to mother plants. Molecular analysis confirmed a high level of genetic stability of blue honeysuckle after 2 years of in vitro propagation. However, among the cultivars studied, ‘Wojtek’ showed slightly higher genetic stability than ‘Zojka’ (99.5% and 97.7%, respectively). For ‘Zojka’ plants, the degree of variation was comparable for AFLP and ISSR markers. For ‘Wojtek’, no polymorphism was detected using the ISSR analysis in contrast to the AFLP analysis.

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“…The genome profiling of plants with pharmaceutical value, using different molecular fingerprinting methods, was reviewed by Gantait et al [ 6 ]. Several of these methods have been applied to address the genetic fidelity of in vitro propagated plants, examples include Hedychium coronarium by inter simple sequence repeats (ISSR) [ 7 ], the endangered medicinal plant Rauwolfia tetraphylla , by start codon targeted polymorphism (SCoT), ISSR, and random amplified polymorphic DNA (RAPD) [ 8 ], Helianthus verticillatus in vitro raised plants using SSRs [ 9 ] and the blackberry ( Rubus fruticosus L.) using RAPD and sequence-related amplified polymorphism (SRAP) [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes

Badr

El-Shazly

Sakr

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Wild medicinal plants are suffering natural environmental stresses and habitat destruction. The genetic diversity evaluation of wild accessions and their in vitro raised genotypes using molecular markers, as well as the estimation of substances of pharmaceutical value in wild plants and their regenerated genotypes are convenient approaches to test the genetic fidelity of regenerated plants as a source of substances of pharmaceutical value. In this study, the genetic diversity of 12 accessions of the medicinal plant Achillea fragrantissima, representing five sites in the mountains of South Sinai, Egypt, were estimated by the inter simple sequence repeats (ISSR) fingerprinting and their volatile oil components were identified using gas chromatography-mass spectrometry (GC-MS) analysis. The same accessions were regenerated in vitro and the genetic diversity and volatile oil components of propagated genotypes were determined and compared to their wild parents. Results Clustering and principal component analyses indicated that the wild accessions and their regenerated genotypes were genetically differentiated, but the regenerated plants are relatively more diverse compared to their wild parents. However, genetic variation between wild accessions is inherited to their in vitro propagated genotypes indicating genotypic differentiation of the examined accessions. The number of volatile oil compounds in the wild A. fragrantissima accessions was 31 compounds while in the in vitro propagated plants only 24 compounds were detected. Four major compounds are common to both wild and regenerated plants; these are artemisia ketone, alpha-thujone, dodecane, and piperitone. Conclusions Genome profiling and essential oil components analysis showed variations in A. fragrantissima accessions from different populations. Genetic differences between wild and regenerated genotypes were analyzed and validated with the final conclusion that in vitro conditions elicited higher genetic variation that is associated with reduced amount and diversity in the essential oil components.

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Evaluation of genetic fidelity of in vitro-propagated blackberry plants using RAPD and SRAP molecular markers

Cited by 27 publications

References 27 publications

Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (Ribes grossularia L.)

Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (Ribes grossularia L.)

Ex vitro Rooting, Acclimatization and Genetic Stability of Lonicera caerulea var. kamtschatica

Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes

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