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Gooseberry (Ribes grossularia L.) is a small fruit crop producing valuable fruits, which is constantly gaining importance. In vitro propagation of this species can significantly support the production of virus-free planting material and accelerate the introduction of new cultivars to the market. The aim of presented study was to assess field performance and genetic stability of micropropagated plants (MPs) of four gooseberry cultivars, “Captivator”, “Hinnonmaki Rot”, “Invicta”, and “Resika”. The growth vigor and yield of MPs and plants propagated by standard methods from softwood cuttings (ST) were evaluated in a field experiment. Microscopic observations of the number and length of the stomata of MP and ST plants were carried out. Two DNA-based techniques, amplified fragment length polymorphism (AFLP) and inter simple sequence repeat (ISSR), were used to assess genetic stability of MP plants. For analysis of genetic stability of ST plants, the ISSR technique was applied. For three cultivars, Captivator, Hinnonmaki Rot, and Invicta, the plants’ growth vigor and fruit yield were greater in MP plants than in ST plants. In the case of Resika, most of these parameters were higher in ST plants. Microscopic observations of the stomata indicated a lack of differences in the length between MP and ST plants, while the stomata frequency on leaves of MP plants was higher than that of ST plants. The genetic variability of MP plants, assessed by AFLP, ranged from 0.35% for Hinnonmaki Rot to 2.12% for Resika. The results of ISSR analysis of MP plants showed variability from 0% in the case of Hinnonmaki Rot and Resika to 4% and 8.69% for Captivator and Invicta, respectively. No polymorphism was detected among ST plants of all analyzed gooseberry cultivars.

The pattern of regeneration from tissues of Paeonia mlokosewitschii and P. tenuifolia cultured in vitro in the same chemical conditions depended on the initial explant. Direct shoot regeneration was obtained from the bases of petioles and petals, and leaf veins. Vegetative initial buds and regenerated in vitro shoots produced on their bases slowly growing nodular callus which was very productive in repetitive shoot regeneration. The tops of stems, flower bases, sepals, petals and ovary walls produced small callus which regenerated white and red spherical structures within 1.5 years. After that time also from those cultures arised nodular, shoot regenerating callus developed

Lisek A., Rozpara E., Głowacka A., Kucharska D., Zawadzka M. (2015): Identification of S-genotypes of sweet cherry cultivars from Central and Eastern Europe. Hort. Sci. (Prague), 42: 13-21.Sweet cherry is a species that is characterized by self-incompatibility, which causes difficulties in obtaining high yields of sweet cherry fruit. Determination of the composition of the S-alleles of sweet cherry cultivars is useful both to growers producing the fruit and breeders when selecting cultivars for cross-fertilizations. In this work, S-alleles have been identified for 47 sweet cherry cultivars from Central and Eastern Europe, mostly from Ukraine and the Czech Republic, with S-genotypes of 43 cultivars identified for the first time. To identify the S-alleles, PCR-based methods were used. In each cultivar, two of the following eight S-alleles were identified: S 1 , S 2 , S 3 , S 4 , S 5 , S 6 , S 9 and S 13 . In the cultivars from the Czech Republic, the most frequently occurring were the alleles S 3 , S 1 and S 4 . In the cultivars originating in Ukraine, the most frequently found were the alleles S 5 and S 9 , which makes them differ considerably from the sweet cherry cultivars from other regions of Europe. The tested sweet cherry cultivars were assigned to 20 of the existing incompatibility groups.

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