Evaluation of genetic integrity of pearl millet seeds during aging by genomic-SSR markers

Dan, Xuming; Wang, Chengran; Su, Yanning; Zhang, Ailing; Wang, Ruijia; Khan, İmran; Huang, Linkai

doi:10.1007/s11033-020-05642-w

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…The electrophoresis results were compared and revised manually. The present bands were labeled as ‘1’, while absent bands were marked as ‘0’ to establish the data matrix in Excel 34 . Genetic diversity was estimated by the number of alleles (Na), effective number of alleles (Ne), Nei’s genetic diversity index (He) and Shannon’s Information index (I) using POPGENE 32.…”

Section: Methodsmentioning

confidence: 99%

Identification and analysis of the genetic integrity of different types of rice resources through SSR markers

Zhou

Jin

et al. 2023

Sci Rep

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Seed aging is the key factor leading to the loss of genetic integrity. In this study, the seeds of Dongxiang wild rice, Xianggu, 9194 and Nipponbare were kept in a plant incubator with constant temperature and humidity for artificial aging treatment. The genetic integrity of germplasm resources with different germination gradients were analyzed using 44 SSR markers. The results suggested that different accessions could be ranked in order of aging resistance from highest to lowest as common wild rice > Xianggu > 9194 > Nipponbare. In order to maintain the genetic diversity of rice, the population size for reproduction and regeneration should be between 60 and 140. After aging, the number of polymorphic alleles, the number of specific single plant, the ratio of polymorphic bands, the number of alleles, the number of effective alleles, gene diversity index and Shannon index of different accessions all decreased with the decrease of germination rate. The germination rate of 60% was the critical value to maintain genetic integrity. Besides, the genetic integrity of eighteen SSR markers was rapidly lost or significantly increased. The regions of these markers were closely related to seed viability or genetic integrity. This study provides a theoretical basis for determining the population size for reproduction and regeneration and the critical value of germination rate of rice resources.

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Section: Methodsmentioning

confidence: 99%

Identification and analysis of the genetic integrity of different types of rice resources through SSR markers

Zhou

Jin

et al. 2023

Sci Rep

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“…Br. ), as the sixth most crucial cereal crops after rice, wheat, maize, barley and sorghum (Sorghum bicolor (L.) Moench) in the world (Awan et al, 2020;Dan et al, 2020;Khan et al, 2020a;Khan et al, 2019a;Khan et al, 2020b;Khan et al, 2019b;Shivhare and Lata, 2016;Sun et al, 2020), is cultivated on ~27 million hectares worldwide as a staple food crop in arid and semi-arid regions of sub-Saharan Africa, India and South Asia where grain yields average 900 kg/ha (Andrews and Kumar, 1992;Varshney et al, 2017). It feeds more than 90 million farmers living in poverty with high nutritious (8-19% protein), high fiber (1.2 g/100 g), low starch, and higher micronutrient concentrations (iron and zinc) than wheat, rice, sorghum and maize (Varshney et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Research on the Drought Tolerance Mechanism of Pennisetum Glaucum (L.) in the Root During Seedling

Zhang

Yang

Sun

et al. 2020

Preprint

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BackgroundDrought, being as one of the major environmental limiting factors, leads a huge reduction in crop growth rate and biomass accumulation. Pearl millet is a suitable material to study drought resistance due to its excellent drought tolerance. Root is a very important part of plant playing crucial roles in plant growth and development, which makes it a target to research on.ResultsIn this study, we tried to find the mechanism of drought tolerance of pearl millet by comparing the data of physiology and transcriptome under normal and drought conditions at 3 time points (1 hour, 3 hours and 7 hours) in root at seedling stage. The relative electrical conductivity went up from 1hour to 7 hours in both control and drought stress groups and the content of malondialdehyde showed a decreasing trend. 2004, 1538 and 605 differently expressed genes were found at 1hour, 3 hours and 7 hours respectively. Some of these differently expressed genes were clustered significantly into ‘metabolic processes’, ‘MAPK signaling pathway’ and ‘plant hormone signal transduction’ like ABA signal transduction pathway in GO and KEGG enrichment analysis.ConclusionsPearl millet was found to have a rapid drought response, which may occur before 1 hour contributing to its drought tolerance. These results can provide a theoretical basis to enhance drought resistance in other plants.

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Genomic survey of MYB gene family in six pearl millet (Pennisetum glaucum) varieties and their response to abiotic stresses

Dan

Yang

et al. 2023

Genetica

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Besides developmental and metabolic aspects, MYB transcription factors play key roles in plant defence and stress responses. From six pearl millet genomes, 1133 MYB genes were identi ed, and four subgroups were constructed by phylogenetically. The duplication pattern of MYB genes in the pearl millet genomes has a conserved and similar evolutionary history. In abiotic stress, MYB genes have a preference for drought stress and heat stress and are more strongly regulated in roots. Multiple analyses have shown that MYB genes respond to abiotic stresses by regulating ABA-related, circadian rhythms and histone modi cation pathways. Under abiotic stress, a large number of duplication events occurred in differentially expressed genes, and the positive expression trend of duplication on PMA5G04432.1-PMA2G00728.1 was consistent across multiple stresses, suggesting that gene duplication is essential for pearl millet adaptation to abiotic stresses. This work will contribute to further studies on the MYB gene family in pearl millet and facilitate further understanding of the potential role of MYB in the regulation of abiotic stresses.

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Evaluation of genetic integrity of pearl millet seeds during aging by genomic-SSR markers

Cited by 5 publications

References 29 publications

Identification and analysis of the genetic integrity of different types of rice resources through SSR markers

Identification and analysis of the genetic integrity of different types of rice resources through SSR markers

Research on the Drought Tolerance Mechanism of Pennisetum Glaucum (L.) in the Root During Seedling

Genomic survey of MYB gene family in six pearl millet (Pennisetum glaucum) varieties and their response to abiotic stresses

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