Evaluation of genetic relationship among varieties of Capsicum annuum L. and Capsicum frutescens L. in West Africa using ISSR markers

Abstract

The taxonomic identity of two closely related Capsicum species; Capsicum annuum and Capsicum frutescens in West Africa has not been clarified because they have overlapping morphological traits. Effective control and management measures as well as improvement of crop plants in any breeding programme can only be implemented when plant species are correctly identified. The genetic relationships of the varieties of these Capsicum species were assessed using 10 ISSR primers for the first time. The varieties and species used include C. annuum var. abbreviatum ; C. annuum var. acuminatum ; C. annuum var. grossum and C. frutescens var. baccatum. PCR amplification of the isolated DNA from the four varieties of Capsicum revealed a total of 75 loci out of which 14 were found to be polymorphic. Average polymorphism information content (PIC) and heterozygosity (He) of the 10 ISSR markers were estimated as 0.67 and 0.78 respectively. The relatedness among the varieties assessed by Unweighted Pair Group Method with Arithmetic Mean (UPMGA) cluster analysis did not separate C. frutescens var. baccatum from the three cultivated varieties of C. annuum . The result from the principal component analysis (PCA) further supports the genetic relatedness and groupings obtained from the cluster analysis. Overall, the study indicated that ISSR markers were effective in assessing the genetic relatedness and revealed genetic homogeneity of the four varieties. Our results, therefore, support the inclusion of C. frutescens var. baccatum as a variety of C. annuum species.

Morphometric variation, genetic diversity and allelic polymorphism of an underutilised species Thaumatococcus daniellii population in Southwestern Nigeria

“…The populations exhibited considerable heterologous amplification of the alleles, which implies that the selected markers are effective in assessing the genetic variation of the populations. This agreed with earlier studies which demonstrated that microsatellite markers are effective in characterization and genotyping of crop plants (Ajibade et al 2000;Rana et al 2014;Animasaun et al 2015, Olatunji andAfolayan 2019). Since a higher number of alleles and high polymorphism help to accurately estimate the genetic diversity of the population, marker profiles are usually interpreted in terms of allele phenotypes (Esslink et al 2004).…”

Genetic State of Pulsatilla Patens (L.) Mill. Coenopopulations Based on DNA Labeling

“…Following the method, the DNA fragments that are characteristic of a certain genus are called "generic" and those that are characteristic of a certain species are called "specific" (Olatunji and Afolayan, 2019). The generic and 164 specific DNA fragments are monomorphic and allow identifying the samples at the specific and generic levels.…”

“…ISSR-PCR markers are dominant; therefore, the presence or absence of this sequence in the genome will be determined by the presence or absence of a discrete band on the electropherogram of the studied sample. This method is widely used for identifying the intra-and interspecific differences, determining the genetic diversity indicators, identifying the varieties and lines and labeling the economically valuable traits (Boronnikova, 2013;Olatunji and Afolayan, 2019;Shahabzadeh et al, 2020).…”

Transferability of ISSR, SCoT and SSR Markers for Chrysanthemum × Morifolium Ramat and Genetic Relationships Among Commercial Russian Cultivars

“…The PIC maximal value for dominant markers is 0.5 [ 26 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]; that is what we obtain as output from the online tool [ 35 ]. On the other hand, some studies reported a PIC value for dominant markers higher than 0.5 [ 36 , 37 , 38 , 39 ].…”