Evaluation of Genetic Similarity and Agronomic Traits of Castor Bean Populations Naturally Grown in the Eastern Mediterranean Region of Turkey

Arslan, Mehmet

doi:10.5504/bbeq.2012.0034

Cited by 7 publications

(6 citation statements)

References 17 publications

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“…Among the molecular markers SSRs and very recently developed CBDP markers are widely used to validate the morphological data 8 . The biochemical markers have been employed in several crops to assess the extent of genetic diversity 9 . Among the biochemical markers, SDS-PAGE shows better validity and ease for ascertaining genetic diversity of crop species and consequently it is employed in a wide range of crops for diversity analysis 10 .…”

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confidence: 99%

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Raina

Laskar

Tantray

et al. 2020

Sci Rep

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cowpea, Vigna unguiculata (L.) Walp. is an important grain legume grown in the dry agro-ecologies of the tropics with considerably low yield due to lack of improved varieties, aggravated by prevalent narrow genetic base. thus, induced mutagenesis was employed using sodium azide and gamma rays to increase genetic variability in cowpea genotypes that resulted in isolation of eleven high yielding mutant lines at the M 4 generation from the genetic background of cowpea varieties Gomati VU-89 and Pusa-578. In order to analyze the induced genetic divergence among the mutant lines and parent genotypes, biochemical and molecular characterization was carried out with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), simple sequence repeat (SSR) and cAAt box derived polymorphism (cBDp) markers. Activity of nitrate reductase (nR) and content of chlorophyll, carotenoid, protein and mineral were found to be significantly high in the selected mutant lines compared to their respective parent genotypes. SDS-PAGE profile of seed proteins generated 54 and 28 polymorphic bands and a total polymorphism of 62.06 and 41.17% in Gomati VU-89 and Pusa-578, respectively. SSR primers amplified a total of 16 and 24 polymorphic bands with an average polymorphism of 20.69 and 50.74% in Gomati VU-89 and Pusa-578, respectively. CBDP markers, used for the first time in mutagenized population, generated 175 bands with 77 bands being polymorphic in Gomati VU-89 and 121 bands with 59 bands being polymorphic in Pusa-578. Physiological, biochemical and molecular profiling of the selected promising mutants lines showed that Gomati VU-89-G and Pusa-578-C are genetically most diverged high yielding genotypes with significant increase in protein and micronutrient content, therefore, could be recommended for further research considerations. thus, the favorable combination of genes induced in the novel cowpea mutants selected through the present study are valuable to correspond farmers requirements for new improved cultivars (direct or hybrids).Pulses are the chief components of the agricultural system, effectively boosting food and nutrition, revenue and environment across the globe and hence assumed ideal for acquiring food security in the developing world including India. Even though India is the leading producer of pulses with 26% of the total world production harvested at about 35% of the total world area, yet a prominent yield gap of 18% exists and one of the leading importer nation 1 . Cowpea [Vigna unguiculata (L.) Walp.] is a vital leguminous crop growing in the semi-arid tropics of Asia, Africa, Southern Europe, Southern United States, and Central and South America 2 . Cowpea leaves, green pods, and grains are excellent dietary source of protein, thus used as food and feed for healthy growth of both humans and livestock 3 . Cowpea is reportedly considered to be first originated and domesticated in Southern Africa, and later spread to east and West Africa and Asia 4 . Worldwide about 6.5 million metric tons of cowpea are produc...

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confidence: 99%

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Raina

Laskar

Tantray

et al. 2020

Sci Rep

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“…The seeds of plants are not only organs of propagation and dispersal but also the major plant tissue harvested by humans. There are significant amounts of protein in the seeds and vary from one type to another, for example, 10% (in grains) 40% (in legumes) of the dry weight, which constitutes a major source of dietary protein (Arslan 2012). Storage proteins are vital because they determine not only the total protein content of seeds but also their quality for different uses (Arslan 2012).…”

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confidence: 99%

“…However, these markers are affected by environmental conditions and have some limitations. Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) of storage seed proteins is the most commonly used method to discriminate the varieties, and it is used for selection and genetic diversity analysis (Arslan 2012) of lentil and other crops. It has been applied to several organisms: bacteria, animals, and plants because t is relatively simple and easy to achieve.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity in several genotypes of Algerian lentil using biochemical markers

et al. 2021

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Abstract. Hammadi H, Hammouda-Bousbia D, Chaib G, Tir A. 2021. Genetic diversity in several genotypes of Algerian lentil using biochemical markers. Biodiversitas 22: 3494-2500. Studying seed storage proteins in legumes (Fabaceae) is very important besides its economic value; seed storage proteins play an important role in plant taxonomy because of the stability of their contents. The grain protein content and protein profiles of 12 lentil genotypes (Lens culinaris Medik.) were characterized based on protein profiling through SDS-PAGE. The obtained gel has shown forty bands ranged from 13 to 21 with molecular weight 10 to 120 KDa. Of which six monomorphic bands, thirty-four polymorphic bands and five unique bands in Flip90-31, Idlep3, idlep2, Idlep4 and Radjas genotypes. The protein banding pattern polymorphism (84%) revealed an inter-genotypic variability. The Idlep3 genotype is characterized by specific bands (or a specific marker), followed by the Flip90-31 genotype. So these two genotypes can be considered as valuable gene resources for further breeding programs.

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“…Because these markers are affected by environmental conditions and have some limitations (Lee, 2006), biochemical or protein markers are widely used in plant breeding and identification (Abdel-Hady and ElNaggar, 2007;Ford et al, 2007;Sipahi et al, 2010). Seed storage protein markers, a type of biochemical marker, can be used for selection (Sönmezoğlu et al, 2010) and genetic diversity analysis (Javaid et al, 2004;Ali et al, 2007;Yüzbaşıoğlu et al, 2008;Arslan, 2012) of lentil and other crops. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), which requires protein markers, is an easy, safe, and efficient method to discriminate lentil cultivars.…”

Section: Introductionmentioning

confidence: 99%

Insights into rootstock biology under low oxygen

Erdoğan¹

2015

Chilean J. Agric. Res.

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Genetic characterization of lentil (Lens culinaris Medik.) cultivars is important for lentil breeding. Therefore, random amplified polymorphic DNA (RAPD) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis were carried out to evaluate genetic diversity in 13 Turkish lentil cultivars. A total of eight RAPD primers were used in this study; 61 bands were produced and 55 of them were polymorphic (89.78%). The RAPD primers OPA-10, OPB-11, and OPI-13 had the highest polymorphism ratio (100%). As a result of SDS-PAGE analysis, variations in the seed protein pattern were observed among the lentil cultivars being studied. The SDS-PAGE similarity matrices indicated higher genetic similarity estimates among the lentil cultivars than RAPD. In addition, principal components analysis (PCA) was performed for both SDS-PAGE and RAPD where the first three components accounted for 75.760% and 68.121% of the total variation for SDS-PAGE and RAPD analysis, respectively. It was noted that the lentil cultivars with factor loadings greater than 0.5 for each principal component (PC) were also grouped together in the SDS-PAGE and RAPD dendrogram. In addition to genetic diversity, cotyledon color (an important market criterion) values were measured for lightness (L*), redness (a*), and yellowness (b*). As for cotyledon color, values for brightness, redness, and yellowness varied significantly among lentil cultivars. Among the red lentil cultivars, 'Çağıl' and 'Yerli Kırmızı' had the highest cotyledon L* values of 70.83 and 70.74, respectively. The results of both genetic diversity analyses and cotyledon color of lentil cultivars would help in planning future breeding programs to improve high yielding marketable lentil cultivars.

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Evaluation of Genetic Similarity and Agronomic Traits of Castor Bean Populations Naturally Grown in the Eastern Mediterranean Region of Turkey

Cited by 7 publications

References 17 publications

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Characterization of Induced High Yielding Cowpea Mutant Lines Using Physiological, Biochemical and Molecular Markers

Genetic diversity in several genotypes of Algerian lentil using biochemical markers

Insights into rootstock biology under low oxygen

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