Evaluation of genetic stability and analysis of phytomedicinal potential in micropropagated plants of Rumex nepalensis – A medicinally important source of pharmaceutical biomolecules

Bhattacharyya, Paromik; Kumaria, Suman; Bose, Biswajit; Paul, Prasenjit; Tandon, Pramod

doi:10.1016/j.jarmap.2017.02.003

Cited by 16 publications

(11 citation statements)

References 59 publications

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“…The genetic changes experienced by the culture include: alternative DNA methylation, amplification, activation of transposable elements, polyploidy, changes in chromosome number or DNA sequence (Bairu et al 2011;Stanišic et al 2015;Govindaraju and Arulselvi 2016). Typically regeneration protocols involving a callus phase are considered the least reliable for clonal propagation, while plantlets regenerated by branching of the axillary buds or direct somatic embryos are considered to be, genetically, the most uniform (Rani and Raina 2000;Varshney et al 2001;Bhattacharyya et al 2017b). The occurrence of somaclonal variations during in vitro propagation, industrial production of phytochemicals, or genetically engineered plants can lead to massive economic consequences and represents a serious obstacle in the practical utilization of plant tissue culture techniques for the production of active metabolites (Rahman and Rajora 2001; ).…”

Section: Improvements In Traditional Culturing Techniquesmentioning

confidence: 99%

In vitro plant tissue culture: means for production of biological active compounds

Espinosa-Leal

Puente-Garza

García‐Lara

2018

Planta

435

206

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Plant tissue culture as an important tool for the continuous production of active compounds including secondary metabolites and engineered molecules. Novel methods (gene editing, abiotic stress) can improve the technique. Humans have a long history of reliance on plants for a supply of food, shelter and, most importantly, medicine. Current-day pharmaceuticals are typically based on plant-derived metabolites, with new products being discovered constantly. Nevertheless, the consistent and uniform supply of plant pharmaceuticals has often been compromised. One alternative for the production of important plant active compounds is in vitro plant tissue culture, as it assures independence from geographical conditions by eliminating the need to rely on wild plants. Plant transformation also allows the further use of plants for the production of engineered compounds, such as vaccines and multiple pharmaceuticals. This review summarizes the important bioactive compounds currently produced by plant tissue culture and the fundamental methods and plants employed for their production.

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Section: Improvements In Traditional Culturing Techniquesmentioning

confidence: 99%

In vitro plant tissue culture: means for production of biological active compounds

Espinosa-Leal

Puente-Garza

García‐Lara

2018

Planta

435

206

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“…Genetic fidelity analysis by SCoT and Ycf1 b DNA barcoding primers In vitro micropropagated plants are known to undergo somaclonal variations in several plant species irrespective of the cultural conditions, choice of explants, and PGRs used (Larkin and Scowcroft 1981;Goto et al 1998;Krishna et al 2016) leading to genetic instability, and validation of true-totype genetic homogeneity remains one of the important prerequisites (Lakshmanan et al 2007). Several molecular markers including RAPD, ISSR, and SSR (Bhattacharyya et al 2017;Tikendra et al 2019) have been extensively utilized for analyzing the clonal/genetic fidelity of micropropagated plants. A highly reproducible and novel molecular marker, start codon targeted (SCoT) marker, based on targeting the short consensus conserved region flanking the ATG translation start codon in plant genes (Collard and Mackill 2009), has been utilized in the present study, which can be correlated to expressible genes and their corresponding traits.…”

Section: Total Phenolics Flavonoids and Antioxidant Potentialmentioning

confidence: 99%

In vitro regeneration, antioxidant potential, and genetic fidelity analysis of Asystasia gangetica (L.) T.Anderson

Dilkalal

Akula

Umesh

2021

In Vitro Cell.Dev.Biol.-Plant

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An effective protocol for the plant regeneration via direct and indirect organogenesis has been developed from leaf explants of Asystasia gangetica (L.), cultured on Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of auxin and cytokinins. Approximately 86% of explants produced direct shoots on MS medium containing 0.5 mg L −1 6-benzyladenine (BA) and 10 μg L −1 Triacontanol (TRIA) with a maximum of 4.82 ± 0.29 shoots per leaf segment. For production of callus-mediated plantlets (indirect), primarily callus was induced on MS medium containing 2 mg L −1 2,4dichlorophenoxyacetic acid (2,4-D), which was then subcultured on medium with 0.1 mg L −1 naphthaleneacetic acid (NAA), 0.5 mg L −1 BA, and 1 to 8 mg L −1 2-isopentenyl adenine (2iP) in order to develop organogenic callus and subsequent shoot induction. A maximum of 6.84 ± 0.05 shoots per callus clump was obtained on MS media supplemented with 4 mg L −1 2iP, 0.5 mg L −1 BA, and 0.1 mg L −1 NAA. The shootlets produced roots when cultured on half-strength MS media supplemented with 2 mg L −1 indole-3-butyric acid (IBA). In vitro propagated plantlets were hardened on soil rite and acclimatized to field condition with 85% survivability. The chlorophyll content of acclimatized plants was comparable with that of the mother plant, while stomatal micromorphology of regenerated plants exhibited no abnormalities. The radical scavenging and antioxidant activity of methanolic extract of leaves were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing ability of plasma (FRAP), and phosphomolybdenum test. In all experiments, regenerated plants exhibited enhanced antioxidant potential indicating micropropagated plants could be exploited for isolation of novel biomolecules. Further, the genetic homogeneity of acclimatized plants was confirmed by PCR-based start codon targeted (SCoT) markers and ycf1b DNA barcoding primers which exhibited monomorphic bands identical to the normal mother plant and no variations were observed.

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“…Combination and concentration of the applied auxin and cytokinine were reported to strongly influence the biosynthesis and accumulation of flavonoids in the cultured plant cell (Bota & Deliu, 2015). Plant tissue culture techniques have been applied to some Rumex species other than R. pictus such as R. vesicarius (Abo El-Soud et al, 2011;El-Shafey et al, 2016 andSayed et al, 2017), the hybrid sorrel Rumex tianschanicus x Rumex patientia (Ślesak et al, 2014), R cyprius (Al Khateeb et al, 2017) and R. nepalensis (Bhattacharyya et al, 2017). However, to our knowledge, there have been no previous reports investigating the in vitro culture of R. pictus.…”

Section: Introductionmentioning

confidence: 99%

Effect of growth regulators on micropropagation, callus induction and callus flavonoid content of Rumex pictus Forssk

et al. 2019

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T HE CURRENT study investigates the effect of growth regulators on micropropagation, callus induction and callus flavonoid content of Rumex pictus Forssk; an endangered medicinal plant. Various combinations of kintin (KIN), 6-benzylaminopurine (BAP), Thidiazuron (TDZ) and indole-3-butyric acid (IBA) were used for micropropagation from shoot tip explants. Murashige and Skoog (MS) medium containing 2mg L-1 BAP was the best for micropropagation where shoot formation frequency was 70% with 12.6 shoots/explant. The highest root formation frequency resulted on half-strength MS medium fortified with 2mg L-1 naphthalene acetic acid (NAA) or 1mg L-1 IBA. One mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 0.4mg L-1 BAP induced the highest callus mass production from the investigated explants, particularly 1 st foliage leaf that was the best explant for callus induction and proliferation. The accumulation of flavonoids in callus was diminished by BAP but enhanced by increasing 2,4-D concentration in culture medium. To our knowledge, this is the first report of micropropagation and callus induction of this endangered medicinal plant which will help in its rapid mass production, conservation and in vitro production of secondary metabolites.

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Evaluation of genetic stability and analysis of phytomedicinal potential in micropropagated plants of Rumex nepalensis – A medicinally important source of pharmaceutical biomolecules

Cited by 16 publications

References 59 publications

In vitro plant tissue culture: means for production of biological active compounds

In vitro plant tissue culture: means for production of biological active compounds

In vitro regeneration, antioxidant potential, and genetic fidelity analysis of Asystasia gangetica (L.) T.Anderson

Effect of growth regulators on micropropagation, callus induction and callus flavonoid content of Rumex pictus Forssk

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