Evaluation of genetic variation in mutants of Black gram (Vigna mungo L.) Hepper) as revealed by RAPD markers

Abstract

One of the most possibilities is the genetic marker system to find out the genetic variation. The advent of large-scale DNA sequencing technology has generated a tremendous amount of sequence information for many important organisms. The genetic variation was evaluated in four mutants (high seed protein, tall, bushy and dwarf mutants) along with parent cultivar (control) by 20 random primers which generated 202 fragments scored with 58 polymorphic DNA bands. The average DNA bands were 10.1 per locus ranged from 1 to 9. The average polymorphic rates were 38.37 % among mutants and parent cultivar (control) through the 20 primers. Primer OPK-06 and OPK-11 revealed 62.5 % DNA polymorphism. Five genotypes were used to constructed dendrogram based on the similarity matrix, suggested that genetic distance from 0.621 to 0.785. The DNA variation might have been caused by mutation due to gamma rays and ethyl methane sulphonate. Hence, research is needed to analyse function of mutated genes for their mutants characters for future prospects because, such mutants and their genes when using in cross breeding/transgenic technologies will found more production in the development of improved crop varieties like high seed protein, lodging resistances, semi dwarf with high yield.

Molecular characterization of urdbean (Vigna mungo) germplasm related to resistance against urdbean leaf crinkle virus

“…The number of polymorphic bands per primer was higher (2) than that obtained by Souframanien and Gopalakrishna (2004), who found 1.8 polymorphic bands per primer but lower than 4.43 to 8.94, which was obtained by Dikshit et al (2007) (Figure 1) Mash-97, Mash-189, Mash-17, Fsd. Local, 95009, 95017, 6036-1, 6036-14, 6065-5, 6036-24, 07M002, 07M003, 07M004, 07M005, 07M006, 07M007 HS 7 5 ES-1, 6049-20, 6036-12, 6036-21, 6065-3, 62027, 95019 in urdbean is comparable to the reports of several RAPD studies by various authors (Ghaffor et al, 2001;Souframanien et al, 2002;Arulbalachandran et al, 2009). The number of bands produced per genotype ranged from 47 to 61 with an average of 53 bands per genotype ( Figure 2).…”

Field Screening and Molecular Characterization of Urdbean Genotypes for Yellow Mosaic Disease

“…The range of polymorphism observed in this study varied from 22.22 to 88.88% (Table 2). The degree of polymorphism observed in this study on urdbean is comparable to reports from earlier investigations (Arulbalachandran et al, 2009;Binyamin et al, 2011). The highest amount of polymorphism (88.88%) was produced by primer OPB-07, whereas the lowest level (22.22%) was seen with primer OPD-07.…”

Induction of chlorophyll mutants in Zingiber officinale Roscoe by Gamma Rays and EMS

“…Most crop improvement programmes of this species are confined to evaluation and selection of naturally occurring variations. One of the most possibilities is the genetic marker system to find out the genetic variation (Arulbalachandran et al, 2009). The mutation breeding provides an alternative means of induction of variation.…”