One of the most possibilities is the genetic marker system to find out the genetic variation. The advent of large-scale DNA sequencing technology has generated a tremendous amount of sequence information for many important organisms. The genetic variation was evaluated in four mutants (high seed protein, tall, bushy and dwarf mutants) along with parent cultivar (control) by 20 random primers which generated 202 fragments scored with 58 polymorphic DNA bands. The average DNA bands were 10.1 per locus ranged from 1 to 9. The average polymorphic rates were 38.37 % among mutants and parent cultivar (control) through the 20 primers. Primer OPK-06 and OPK-11 revealed 62.5 % DNA polymorphism. Five genotypes were used to constructed dendrogram based on the similarity matrix, suggested that genetic distance from 0.621 to 0.785. The DNA variation might have been caused by mutation due to gamma rays and ethyl methane sulphonate. Hence, research is needed to analyse function of mutated genes for their mutants characters for future prospects because, such mutants and their genes when using in cross breeding/transgenic technologies will found more production in the development of improved crop varieties like high seed protein, lodging resistances, semi dwarf with high yield.

In recent years, the demand of chilli has tremendously increased due to its attractive market price and multifarious used in cooked and processed forms. At present people are much concerned about the fruit quality and yield. Therefore, attention is being paid for development of genotypes having high yield potential with desirable fruit quality characters in a short period of time. For this purpose, seeds of chilli were mutagenised with ethyl methane sulphonate (EMS) and diethyl sulphate (DES) to determine their mutagenic sensitivity in M 1 generation. The increasing concentration of EMS and DES decreased in morphological and yield characters. The spectrum of mutation and induced variability for various quantitative traits were observed in M 1 generation such as germination (%), plant height, primary and secondary branches per plant, days to first flowering, fruit length (cm), fruit girth (cm), total number of fruits per plant, number of seeds per fruit, seed weight per fruit (g), 100 seed weight (g) and pericarp: seed ratio showed variability in chilli with the effect of EMS and DES. The percentage of chromosomal abnormalities in different mitotic stages was significantly higher than that of the control in all the treatment concentrations.

The present study was conducted in order to determine the effect of gamma rays and EMS on seed germination, Seedling height and root length in chick pea to identify the lethal dose (LD 50 ). In this regard, the healthy seeds of chick pea was subjected to different doses/concentrations of gamma rays (20, 30, 40, 50 and 60kR) and EMS (10, 20, 30, 40 and 50mM) for inducing mutation. The effect of gamma rays and EMS was determined by measuring the seed germination, seedling height and root length under the conditions of the M 1 generation. The results shows that, the seed germination, seedling height and root length were significantly decreased with increasing doses/concentrations. The LD 50 values were observed based on the growth reduction of seedlings after treatments with mutagen. The effective doses/concentrations which caused 50% growth reduction were observed in 40kR in gamma rays and 30mM in EMS.

The effect of gamma irradiation and EMS treatment on seed germination and seedling height of Chick pea (Cicer arietinum. L.). In this regard Co-4 variety of chick pea was subjected to different doses/concentrations of gamma rays (20, 30, 40, 50, and 60 kR) and EMS (10, 20, 30, 40 and 50 mM) for inducing mutation. The effect of mutagen was observed on the basis of percentage of seed germination, seedling height reduction at 15 th day and survivability. From the result it was observed that, the percentage of seed germination, seedling height reduction at 15 th day and survivability were significantly decreased with increasing doses/concentrations of mutagen. The effective doses/ concentrations which caused 50 % growth reduction were 40kR in gamma rays and 30 mM in EMS.

The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K 1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M 3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands.