Evaluation of Gingival Crevicular Fluid Adrenomedullin and Human Neutrophil Peptide 1–3 Levels of Patients With Different Periodontal Diseases

Türkoğlu, Oya; Emingil, Gülnur; Kütükçüler, Necil; Atilla, Gül

doi:10.1902/jop.2009.090517

Cited by 27 publications

(42 citation statements)

References 46 publications

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“…They showed that, the total amount of gingival crevicular fluid adrenomedullin in periodontitis sites was higher than that of healthy sites. Similarly, our data was supported by Oya et al (26) They demonstrated that patients with periodontitis had a higher total amount of GCF adrenomedullin compared to healthy controls. Also, they added the total amount of gingival crevicular fluid adrenomedullin was significantly higher in periodontitis groups than that of the gingivitis group, and although not significant, there was a higher total amount of gingival crevicular fluid adrenomedullin in the gingivitis group compared to that of the healthy group.…”

Section: Discussion:-supporting

confidence: 90%

Clinical and Laboratory Evaluation of Adrenomedullin as a Biomarker in Moderate Chronic Periodontitis.

Salim¹,

Youssef²,

Salah³

et al. 2017

IJAR

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Section: Discussion:-supporting

confidence: 90%

Clinical and Laboratory Evaluation of Adrenomedullin as a Biomarker in Moderate Chronic Periodontitis.

Salim¹,

Youssef²,

Salah³

et al. 2017

IJAR

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“…In the present study, no significant difference was found in GCF proteinase 3 levels among the diseased groups. Our previous results demonstrated that the level of GCF HNP1-3 was not different among the patients with gingivitis, generalized aggressive periodontitis, and chronic periodontitis [48]. Besides, we reported that chronic periodontitis patients had significantly higher GCF LL-37 levels compared to healthy controls [49].…”

Section: Discussionmentioning

confidence: 98%

Are Proteinase 3 and Cathepsin C Enzymes Related to Pathogenesis of Periodontitis?

Türkoğlu

Azarsız

Emingil

et al. 2014

BioMed Research International

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Aim. Cathepsin C is the activator of the polymorphonuclear leukocyte-derived proteinase 3, which contributes to inflammatory processes. The aim of the present study was to investigate gingival crevicular fluid (GCF) proteinase 3 and cathepsin C levels in periodontal diseases. Design. Eighteen patients with chronic periodontitis (CP), 20 patients with generalized aggressive periodontitis (G-AgP), 20 patients with gingivitis, and 18 healthy subjects were included in the study. Periodontal parameters including probing depth, clinical attachment level, papilla bleeding index, and plaque index were assessed in all study subjects. GCF proteinase 3 and cathepsin C levels were analyzed by ELISA. Results. GCF proteinase 3 total amount was significantly higher in diseased groups compared to control group, after adjusting age (P < 0.05). No differences were found in GCF cathepsin C levels among the study groups (P > 0.05). Periodontal parameters of sampling sites were positively correlated with GCF proteinase 3 total amounts (P < 0.01) but not with cathepsin C total amounts (P > 0.05). Conclusions. Elevated levels of GCF proteinase 3 in CP, G-AgP, and gingivitis might suggest that proteinase 3 plays a role during inflammatory periodontal events in host response. However, cathepsin C in GCF does not seem to have an effect on the pathogenesis of periodontal diseases.

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“…This resulted from an assumption that with active inflammation, an increase in fluid volume could give a false reading of the dilution values of the studied molecule (only locally present) or its elevated concentration when it is simultaneously expressed in the peripheral blood (Griffiths 2003). Other authors also suggest higher reliability of results expressed as the amount per measure point rather than concentration (Lamster et al 1986; Lamster and Ahlo 2007; Türkoğlu et al 2010). …”

Section: Discussionmentioning

confidence: 99%

“…We assessed HNP1-3 after nonsurgical periodontal therapy; since to our knowledge, such assessments had never been undertaken before and researchers focused mainly on their presence in healthy or diseased periodontium (Lundy et al 2005; McKay et al 1999; Puklo et al 2008; Rangé et al 2012; Türkoğlu et al 2010). A study conducted by Lundy et al (2005) proved higher levels of HNP1-3 in GCF in healthy sites (without statistical significance), which they associated with intact defensin barrier in the state of health.…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Nonsurgical Periodontal Therapy on HNP1-3 Level in Gingival Crevicular Fluid of Chronic Periodontitis Patients

Dolińska

Skurska

Pietruska

et al. 2017

Arch. Immunol. Ther. Exp.

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The rich bacterial flora of oral cavity is controlled by innate immune response, including antibacterial peptides and among them human neutrophil peptides 1–3 (HNP1-3). The knowledge of the involvement of HNPs in innate and acquired immunity of the periodontium is fragmentary. The aim of the study was to assess alterations in HNP1-3 levels in the gingival crevicular fluid (GCF) of chronic periodontitis patients before and after nonsurgical periodontal therapy. Nineteen patients with chronic periodontitis were qualified to the study. After periodontal examination, one site with pocket depth (PD) ≥4 mm was selected. All the patients received periodontal treatment involving scaling and root planing with additional systemic antibiotic therapy (Amoxicillin 375 mg three times daily and Metronidazole 250 mg three times daily for 7 days). Prior to therapy, 3 and 6 months after it, clinical periodontal parameters were measured and GCF was collected from previously chosen site. The level of HNP1-3 in GCF was determined by means of a commercially available enzyme-linked immunoassay kit. The periodontal therapy caused a statistically significant (p < 0.001) decrease in all the assessed clinical parameters at the sites of sample collection except for bleeding on probing. The level of HNP1-3 per measure point showed a statistically significant increase (baseline—3 months: p = 0.05, baseline—6 months: p = 0.007). Within the limits of the study, it can be stated that nonsurgical periodontal therapy with additional systemic administration of Amoxicillin and Metronidazole increases the level of HNP1-3 in GCF.

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Evaluation of Gingival Crevicular Fluid Adrenomedullin and Human Neutrophil Peptide 1–3 Levels of Patients With Different Periodontal Diseases

Abstract: Our results suggest a defensive role for adrenomedullin during the host response in periodontal disease. Additionally, the lack of the HNP1-3 antimicrobial peptide might point to the deficiency of one of the protective mechanisms for periodontal tissues.

Cited by 27 publications

References 46 publications

Clinical and Laboratory Evaluation of Adrenomedullin as a Biomarker in Moderate Chronic Periodontitis.

Clinical and Laboratory Evaluation of Adrenomedullin as a Biomarker in Moderate Chronic Periodontitis.

Are Proteinase 3 and Cathepsin C Enzymes Related to Pathogenesis of Periodontitis?

The Effect of Nonsurgical Periodontal Therapy on HNP1-3 Level in Gingival Crevicular Fluid of Chronic Periodontitis Patients

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