Evaluation of Glucosidase Inhibitory and Cytotoxic Potential of Five Selected Edible and Medicinal Ferns

Chai, Tsun-Thai; Yeoh, Loo Yew; Ismail, Nor Ismaliza Mohd; Ong, Hwai Chyuan; Manan, Fazilah Abd; Wong, Fai-Chu

doi:10.4314/tjpr.v14i3.13

Cited by 29 publications

(21 citation statements)

References 28 publications

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“…Plant extracts with cytotoxic actions are also therapeutically useful for diabetic treatment. 24,25 In line with these, the essential oil and other constituent of H. abyssinica has confirmed the cytotoxic effect in vitro. 21,26 As a result, these Phytoconstituents may contribute to antidiabetic activity.…”

Section: Introductionsupporting

confidence: 59%

Evaluation of in vitro and in vivo Anti-Diabetic, Anti-Hyperlipidemic and Anti-Oxidant Activity of Flower Crude Extract and Solvent Fractions of Hagenia Abyssinica (Rosaceae)

Kifle¹,

Yesuf²,

Atnafie³

2020

JEP

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Background: The flower of Hagenia abyssinica (Rosaceae) has been used in traditional health systems to treat diabetes mellitus in Ethiopia and Tanzania. However, the antidiabetic activity of this medicinal plant is not scientifically validated and authenticated. The present study aimed to investigate the in vitro and in vivo anti-diabetic activity of flower crude extract and solvent fractions of Hagenia abyssinica. Methods: The in vitro α-amylase inhibition and antioxidant activity of the crude extract and solvent fractions of Hagenia abyssinica were evaluated by using 3,5-dinitrosalicylic acid (DNSA) and diphenyl-2-picrylhydrazyl (DPPH) assay model, respectively. Blood glucose lowering activity of 80% methanolic flower crude extract and solvent fraction was studied in four animal models: normoglycemic mice model, oral glucose loaded mice model, single dose-treated streptozotocin-induced diabetic mice model, and repeated dose-treated streptozotocin-induced diabetic mice model. The effect of the crude extract and solvent fraction of Hagenia abyssinica on diabetic lipid profile and body weight was also studied. Results: The acute toxicity study of Hagenia abyssinica flower extract did not show mortality in the animals at the limit dose of 2g/kg during the observation period. The result of α-amylase enzyme inhibition activity was found in a dose-dependent manner, the strongest activity was shown by ethyl acetate fraction (54.23% inhibition at 800 μg/mL) compared to the standard acarbose having 91.87% inhibition at 800 μg/mL. Among these extracts, the crude extract had the highest antioxidant activity (58.38% inhibition at 500 μg/mL). The crude extract of H. abyssinica showed significant blood glucose-lowering effect on normoglycemic mice and oral glucose loaded mice. In streptozotocin-induced diabetic mice model, the crude extract and ethyl acetate fraction significantly decreased the fasting blood glucose level after 14 days of treatment. There were significant reductions in serum total cholesterol, serum triglycerides, very low-density lipoprotein, and low-density lipoprotein. However, there were significant increments in body weight and high-density lipoprotein as compared to untreated diabetic mice. Conclusion: The result demonstrated the beneficial biochemical effects of Hagenia abyssinica extract by inhibiting α-amylase, scavenging diphenyl-2-picrylhydrazyl (DPPH) and improving serum lipid profile levels. The flower crude extract and solvent fractions of Hagenia abyssinica are effective in lowering blood glucose levels in diabetic and normoglycemic mice. The claimed traditional use as antidiabetic has scientific ground.

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Section: Introductionsupporting

confidence: 59%

Evaluation of in vitro and in vivo Anti-Diabetic, Anti-Hyperlipidemic and Anti-Oxidant Activity of Flower Crude Extract and Solvent Fractions of Hagenia Abyssinica (Rosaceae)

Kifle¹,

Yesuf²,

Atnafie³

2020

JEP

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“…These findings indicate that pterosin A may be a viable therapeutic option for diabetes (Hsu et al 2013). Chai et al (2015a) reported that an aqueous extract of P. vittata fronds exhibited moderate, dose-dependent antiglucosidase activity (EC 50 87 µg/mL) when compared to myricetin (EC 50 53 µg/mL). The identity of the alpha-glucosidase inhibitors in the extract is unknown.…”

Section: Antidiabetic Activitymentioning

confidence: 86%

“…-[4-hydroxy-(E)-cinnamoyl]-glucopyranosyl-(1 → 2)-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside Ternatumoside XVKaempferol 3-O-[β-D-xylopyranosyl-(1 → 4)]-[β-D-6-O-[4-hydroxy-(E)-cinnamoyl]-glucopyranosyl-(1 → 3)]-β-D-6-O-[4-hydroxy-(E)-cinnamoyl]-glucopyranosyl-(1 → 2)-α-Lrhamnopyranoside-7-O-β-D-glucopyranoside Ternatumoside XVI Kaempferol 3-O-[β-D-glucopyranosyl-(1 → 4)]-[β-D-6-O-[4-hydroxy-(E)-cinnamoyl]-glucopyranosyl-(1 → 3)]β-D-6-O-[4-hydroxy-(E)-cinnamoyl]-glucopyranosyl-(1 → 2)-α-L-rhamnopyranoside-7-O-β-D-glucopyranoside Ternatumoside XVII Kaempferol 3-O-[β-D-6-O-[4-hydroxy-(E)-cinnamoyl]-glucopyranosyl-(1 → 3)]-β-D-6-O-[4-hydroxy-(E)cinnamoyl]-glucopyranosyl-(1 → 2)-α-L-rhamnopyranoside-7-O-α-D-glucopyranosyl-(1 → 6)-β-Dglucopyranosidethis species are cytotoxic towards K562 leukaemic cells(Chai et al 2015a). P. multifida is another arsenic hyperaccumulator that is widely distributed in China and other Asian countries.…”

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confidence: 99%

Phytochemicals from fern species: potential for medicine applications

et al. 2017

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Ferns are an important phytogenetic bridge between lower and higher plants. Historically they have been used in many ways by humans, including as ornamental plants, domestic utensils, foods, and in handicrafts. In addition, they have found uses as medicinal herbs. Ferns produce a wide array of secondary metabolites endowed with different bioactivities that could potentially be useful in the treatment of many diseases. However, there is currently relatively little information in the literature on the phytochemicals present in ferns and their pharmacological applications, and the most recent review of the literature on the occurrence, chemotaxonomy and physiological activity of fern secondary metabolites was published over 20 years ago, by Soeder (Bot Rev 51:442–536, 1985). Here, we provide an updated review of this field, covering recent findings concerning the bioactive phytochemicals and pharmacology of fern species

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“…Thus, this led to that drugs that can improve glycemic index and/or oxidative stress will be beneficial in the treatment of diabetes mellitus and its complications [ 19 , 20 ]. In addition substances with cytotoxic activities are also therapeutically recommended for their antidiabetic effect [ 21 , 22 ]. In line to these, the bioactive constituents of Indigofera spicata has verified cytotoxic and antioxidant activities in vitro [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of hydroalcoholic leaves extract of Indigofera spicata Forssk. on blood glucose level of normal, glucose loaded and diabetic rodents

Birru

Abdelwuhab

Shewamene

2015

BMC Complement Altern Med

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BackgroundDiabetes mellitus is found in all parts of the world and is rapidly increasing in its coverage with alarming rate especially in Asia and Africa. Research is increasingly done with the aim of developing a relatively safe and efficacious anti-diabetic plant based products. Parallelly, this investigation was carried out to evaluate the effect of the hydro alcoholic leaves crude extract of Indigofera spicata (ISP) on the blood glucose level(BGL) of normoglycemic, oral glucose loaded and alloxan induced diabetic rodents.MethodsThe animals were randomly divided into five groups (n = 6) for all the aforementioned three models. In all models, group-I mice provided 2%tween-80, group-II were treated with 5 mg/kg glibenclamide and the remaining three groups (III, IV & V) were treated with 100, 200, and 400 mg/kg dose of the extract respectively. Statistical significance of differences in BGLs within and between groups was analyzed by SPSS version-21 using one way ANOVA followed by Tukey’s post hoc multiple comparison.Result200 mg/kg and 400 mg/kg extract treated groups of normoglycemic mice showed significant (p < 0.05) BGL reduction compared to the pre-exposure level. In case of OGTT model BGL reduction was statistically significant (p < 0.05) in only 400 mg/kg exposed groups at the 120 min of post-exposure compared to the initial level. However, the BGL reducing effect of doses of the extract at the 4th, 6th and 10th hours of post treatment on diabetic mice was found statistically significant compared to both the negative control (p < 0.001) and their respective pretreatment levels (p < 0.05).ConclusionAs it is claimed in ethnobotanical studies, the hydroalcoholic crude extract of ISP leaves have shown prominent anti-diabetic effect and can be therefore used as a good insight for new anti-diabetic drug source with a call for further studies.

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Evaluation of Glucosidase Inhibitory and Cytotoxic Potential of Five Selected Edible and Medicinal Ferns

Cited by 29 publications

References 28 publications

<p>Evaluation of in vitro and in vivo Anti-Diabetic, Anti-Hyperlipidemic and Anti-Oxidant Activity of Flower Crude Extract and Solvent Fractions of <em>Hagenia Abyssinica</em> (Rosaceae)</p>

<p>Evaluation of in vitro and in vivo Anti-Diabetic, Anti-Hyperlipidemic and Anti-Oxidant Activity of Flower Crude Extract and Solvent Fractions of <em>Hagenia Abyssinica</em> (Rosaceae)</p>

Phytochemicals from fern species: potential for medicine applications

Effect of hydroalcoholic leaves extract of Indigofera spicata Forssk. on blood glucose level of normal, glucose loaded and diabetic rodents

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