Evaluation of Green Synthesis of Ag Nanoparticles Using Eruca sativa and Spinacia oleracea Leaf Extracts and Their Antimicrobial Activity

Abstract

Background: Nano-biotechnology is considered as one of the main vigorous fields of research in new material science. Recently, biosynthetic methods employing both biological microorganisms such as bacteria and fungus or plant extracts have developed rapidly as a trouble-free and feasible choice to obtain nanomaterials alternative to more complex chemical synthetic procedures. The particular distinctiveness like size, allocation and shape give the nanoparticles different properties from bulk material. Objectives: Eruca sativa and Spinacia oleracea plants were evaluated for their extra cellular potential synthesis of silver nanoparticles and their bactericidal impact on Streptococcus pneumoniae and Pseudomonas aeruginosa. Materials and Methods: Aqueous solutions of AgNO 3 were mixed with plant extracts. Transmission electron microscopy (TEM) was used to characterize the morphology of the nanoparticles obtained from plant extracts. Energy dispersive X-ray (EDX) spectrometer established the existence of elemental sign of the silver and homogenous allocation of silver nanoparticles. Diffraction by using X ray (XRD) analysis for the formed AgNPs revealed spherical plus cubical structures with different planes ranging between 111 to 311 planes. Scanning electron microscopy (SEM) was used to characterize the morphology of the nanoparticles obtained from plant extracts. Results: The antibacterial action of AgNPs against human pathogens, Streptococcus pneumoniae and Pseudomonas aeruginosa was recognized. Our work showed a rapid, eco-safe and suitable method for the synthesis of AgNPs from Eruca sativa and Spinacia oleracea leaf extracts and can be used for pharmaceutical and other biomedical applications. Conclusions: It can be suggested that AgNPs show effective antibacterial properties owing to their exceptionally big exterior region, which provides superior contact with microorganisms and its interactions with bacteria are and localized on the membrane of the organism.

The prophylactic effect of Ranunculus laetus (Wall)-mediated silver nanoparticles against some Gram-positive and Gram-negative bacteria

“…Furthermore, XRD confirmed their crystalline nature with face-centered cubic lattice and SEM revealed their spherical shape. A similar type of results was found in the case of Eruca sativa and Spinacia oleracea-mediated AgNPs (Alaraidh et al 2014), mulberry AgNPs and Murraya koenigii AgNPs (Vinothkumar et al 2014). The smaller size of silver nanoparticles leads to augmented membrane permeability and cell damage (Ankanna and Savithramma 2011).…”

Green synthesis, characterization and photocatalytic applications of silver nanoparticles using Diospyros lotus

“…To switch over these technical problems, biological methods have been employed using microorganisms and green plant extracts that are eco-friendly and cost effective alternatives to chemical and physical methods [32]. The main problem of using microorganism to synthesize Ag NPs is that it is a very time consuming process in comparison with plant extracts and special culture preparation and isolation techniques are required [33].…”

Green Nanoparticles for Mosquito Control

“…The silver and gold nanoparticles using A. calamus [ 6 ], E. sativa and S. oleracea [ 7 ], T. conoides [ 8 ], and A. nilotica [ 9 ] have been synthesized.…”