Evaluation of Haemagglutination Assay (HA) for the Detection of Peste des Petits Ruminants Virus (PPRV) in Faecal Samples of Recovered Goats

Latif, Asma

doi:10.14737/journal.rjvp/2014/2.1s.11.13

Cited by 5 publications

(8 citation statements)

References 8 publications

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“…Higher heamagglutination titres were recorded with the use of 1% chicken RBCs suspension as described previously by Latif et al (2014) instead of using 0.6% as described earlier by Ezeibe et al (2004), Osman et al (2008) and Wosu (1985Wosu ( , 1991. In addition, higher HA titres were obtained by incubating the plates at 4°C and at time ranging from 17 to 18 min as reported by Osman et al (2008) and Wosu (1985Wosu ( , 1991.…”

Section: Discussionmentioning

confidence: 64%

“…Recently, the HA test was proved to be as sensitive as an immuno-capture enzyme-linked immunosorbent assay (IC-ELISA) for detection of PPRV in nasal swabs and fecal samples from clinically TA B L E 1 Detection of peste des petits ruminants virus antigen in pneumonic camel lung samples by haemagglutination (HA) test PPR suspected camels in Pakistan (Rahman et al, 2020). The HA test can be done using samples from dead or live animals including swabs (ocular, nasal and oral), faecal and various tissue samples to detect the presence of PPRV antigen (Abubakar et al, 2012;Latif et al, 2014;Liu et al, 2013;Manoharan et al, 2005;Zakian et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Higher heamagglutination titres were recorded with the use of 1% chicken RBCs suspension as described previously by Latif et al. (2014) instead of using 0.6% as described earlier by Ezeibe et al. (2004), Osman et al.…”

Section: Discussionmentioning

confidence: 99%

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Detection of peste des petits ruminants virus in pneumonic lungs from clinically apparently healthy camels slaughtered at Tambul slaughterhouse, Central Sudan

Abdalla

Alhussain

Mohammed³

et al. 2021

Veterinary Medicine & Sci

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

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Detection of peste des petits ruminants virus in pneumonic lungs from clinically apparently healthy camels slaughtered at Tambul slaughterhouse, Central Sudan

Abdalla

Alhussain

Mohammed³

et al. 2021

Veterinary Medicine & Sci

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“…rRT-PCR is considered a highly specific test for detection of PPRV [ 11 ]. However, a previous study evaluating HA for detection of PPRV in faecal samples of sheep and goats reported low specificity of HA for detection of PPRV compared to reverse transcriptase PCR [ 20 ]. Therefore, HA may not be considered a suitable test for detection of PPRV antigen in faecal samples of PPR recovered goats.…”

Section: Discussionmentioning

confidence: 99%

Detection of Peste des Petits Ruminants Viral RNA in Fecal Samples of Goats after an Outbreak in Punjab Province of Pakistan: A Longitudinal Study

Ullah

Zahur²,

Latif³

et al. 2016

BioMed Research International

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Peste des petits ruminants (PPR) is a highly contagious viral disease of domestic and wild small ruminants and thus has serious socioeconomic implications. In Pakistan, during the year 2012-2013, estimated losses due to PPR were worth Rs. 31.51 billions. Close contact between infected and susceptible animals is an important route of transmission of PPR. Therefore, carrier animals play an important role in unnoticed transmission of PPR. The objective of the study was to investigate the detection of PPR virus in goats recovered from PPR. A suspected PPR outbreak was investigated and confirmed as PPR after analysing appropriate samples collected from infected animals using rRT-PCR. A longitudinal study was conducted over the period of 16 weeks to ascertain the detection of PPR virus (PPRV) in faecal samples of recovered goats. Ninety-six (96) faecal samples from each sampling were collected at 4, 8, 12, and 16 weeks after the outbreak. Faecal samples were analysed using rRT-PCR. Of 96 from each sampling a total of 46, 37, 29, and 25 samples were positive for PPR viral genome at 4, 8, 12, and 16 weeks, respectively, after recovery. Attempts were made for the isolation of PPR virus on Vero cells, but results were negative. These results indicated the detection of PPR viral RNA up to 16 weeks after infection. Therefore, these results may help in the future epidemiology of PPR virus shedding and possible role as source of silent infection for healthy animals especially when there is no history of any outbreak in nearby flock or area.

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“…Diagnosis of PPR infection is mostly achieved by non-molecular techniques, such as detection of PPRV in blood serum by enzyme linked immunosorbent assay (ELISA) (Lawal et al, 2011) or in faeces using haemagluttination assay (HA) or haemagluttination inhibition (HI) assay (Ezeibe et al, 2004(Ezeibe et al, , 2008Latif et al, 2014;Islam et al, 2018;Osman et al, 2018) or by classical virus isolation (VI) from post-mortem tissues and detection by agar gel immunodiffusion (El-Yuguda et al, 2008). Among the non-molecular techniques, HA is the preferred diagnostic tool because of its rapidity, simplicity and cost effectiveness (Wosu, 1985(Wosu, , 1991Ezeibe et al, 2004;Osman et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Comparative study of molecular and non-molecular tools for peste des petits ruminants virus detection in West African Dwarf goats

Muritala

Bemji

Ozoje

et al. 2023

Preprint

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Peste des petits ruminants (PPR) causes severe economic losses to many countries of the world where the disease is endemic. It has been targeted for global eradication by 2030 following the successful eradication of rinderpest in 2011. The proposed eradication program would benefit from efficient and relatively reliable diagnostic tools for early PPR virus (PPRV) detection. A total of 33 eight to 12 months old West African Dwarf (WAD) goats were used. Nineteen goats infected by comingling with two PPR virus positive animals formed the infected group (PPRV-infected goats) while 14 non-infected goats formed the control group (CTG). The suitability of a molecular method (Hydroxyl naphthol blue (HNB) staining of reverse transcription loop mediated isothermal amplification (RT-LAMP)) and a non-molecular tool (haemagluttination assay (HA)) were compared for their sensitivity to detect the PPRV in PPRV-infected goats and non-infected CTG. PPR disease severity in WAD goats at different days post infection (dpi) was evaluated by clinical scoring and haemagluttination titre (HAT). HNB staining RT-LAMP reaction and HA showed sensitivities of 100% and 73.68%, respectively, for PPRV detection. Expression of PPR clinical signs began from 3 dpi, attained peak at 5 dpi, thereafter showed irregular patterns till 24 dpi. Evaluation of HAT in PPRV-infected goats at 12 dpi ranged from 2 to 64 haemagluttination units (HAU), while CTG goats had 0 HAU. In conclusion, HNB staining RT-LAMP assay demonstrated reasonable potential for accurate diagnoses of PPRV and as an important diagnostic tool in areas with poor electricity supply and less sophisticated laboratory equipment.

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Evaluation of Haemagglutination Assay (HA) for the Detection of Peste des Petits Ruminants Virus (PPRV) in Faecal Samples of Recovered Goats

Cited by 5 publications

References 8 publications

Detection of peste des petits ruminants virus in pneumonic lungs from clinically apparently healthy camels slaughtered at Tambul slaughterhouse, Central Sudan

Detection of peste des petits ruminants virus in pneumonic lungs from clinically apparently healthy camels slaughtered at Tambul slaughterhouse, Central Sudan

Detection of Peste des Petits Ruminants Viral RNA in Fecal Samples of Goats after an Outbreak in Punjab Province of Pakistan: A Longitudinal Study

Comparative study of molecular and non-molecular tools for peste des petits ruminants virus detection in West African Dwarf goats

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