Evaluation of HemogloBind™ for Removal of o-Raffinose Cross-Linked Hemoglobin (Hemolink™) from Serum

Balion, Cynthia; Champagne, Patricia A.; Ali, Arlene

doi:10.1093/clinchem/43.9.1796

Cited by 8 publications

(2 citation statements)

References 7 publications

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“…HemogloBind binds Hb unspecifically by a combination of its polymer composition, charge properties and cross-linking architecture [23]. Apparently, the physiochemical properties of Hb and Hb-Hp causes HemogloBind to preferentially bind Hb and not Hb-Hp, as shown in western blots of HemogloBind-treated plasma samples and also reported previously [24].…”

Section: Discussionsupporting

confidence: 58%

Using the hemoglobin-binding Staphylococcus aureus protein IsdH to enable plasma analysis of hemolyzed blood samples

Sæderup

Revsholm

Richardt

et al. 2019

Clinical Chemistry and Laboratory Medicine (CCLM)

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Background Intravascular hemolysis and in vitro hemolysis are prevalent contributors to failed blood sample analysis in the routine hospital laboratory. Interferences by hemoglobin in spectrophotometric and certain enzyme activity assays is the major causative factor. Methods By exploiting the hemoglobin-binding properties of the iron-regulated surface determinant H (IsdH) protein from Staphylococcus aureus we have developed a new method to instantly remove hemoglobin and hemoglobin-haptoglobin complexes from plasma in vitro thereby enabling the measurement of hemoglobin-sensitive analytes in hemolyzed plasma. In the present study we used an engineered IsdH mutant form conjugated to Sepharose for the efficient removal of plasma hemoglobin in concentrations up to 15 mg/mL. The high abundance of haptoglobin, which forms a tight complex with hemoglobin in plasma, did not affect the hemoglobin removal by IsdH Sepharose. Results Applying the method on plasma samples that beforehand were spiked with blood hemolysate re-enabled measurement of the hemolysis sensitive parameters: alkaline phosphatase, conjugated bilirubin, iron, ferritin, γ-glutamyltransferase, total thyroxine and troponin T. IsdH Sepharose-mediated hemoglobin removal also enabled measurement of hemolysis sensitive parameters in hemolyzed samples from anonymized patients. Conclusions In conclusion, IsdH Sepharose is a simple cost-effective pretreatment of hemolyzed samples correcting and enabling the measurement of several important hemoglobin-sensitive parameters in a way compatible with standard procedures in routine laboratories.

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Section: Discussionsupporting

confidence: 58%

Using the hemoglobin-binding Staphylococcus aureus protein IsdH to enable plasma analysis of hemolyzed blood samples

Sæderup

Revsholm

Richardt

et al. 2019

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…Thus, we focused on aldehydes with a small molecular weight. Such aldehydes readily diffuse in solution and show high reactivity with amino groups in proteins, even under physiological condition . We therefore considered that a small molecule containing multiple aldehyde groups could efficiently crosslink atelocollagen molecules dissolved in physiological saline.…”

Section: Resultsmentioning

confidence: 99%

Sugar-based crosslinker forms a stable atelocollagen hydrogel that is a favorable microenvironment for 3D cell culture

Kamimura

Koyama

Miyata

et al. 2014

J. Biomed. Mater. Res.

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We created sugar-based crosslinkers for precise chemical crosslinking of atelocollagen to form a stable hydrogel microenvironment for three-dimensional (3D) cell culture. Crosslinkers were synthesized by partially oxidizing glucose, fructose, maltose, sucrose, or raffinose with periodate. The partial oxidization of sugar generated multiple aldehydes in the molecule, which acted as multifunctional crosslinkers, allowing atelocollagen to form chemical hydrogels. The crosslink reaction of atelocollagen was competitively suppressed by the addition of amino acid-rich medium, enabling flexible control of 3D molecular density in the acquired hydrogel. To evaluate structural stability, ultrasonic stress was loaded onto the acquired hydrogel and sequential mea-surement of water content showed that the crosslinking considerably stabilized the hydrogel structure. The effectiveness of the atelocollagen hydrogel as a 3D culture scaffold was analyzed by embedding and culturing endothelial cells or cardiomyocytes in it. Endothelial cells formed 3D capillary-like structures at 12 h, and cardiomyocytes formed a beating 3D netlike structure by 7 days. These findings suggest that crosslinked atelocollagen hydrogel effectively functions as a stable scaffold in 3D culture. V C 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 4309-4316, 2014. How to cite this article: Kamimura W, Koyama H, Miyata T, Takato T. 2014. Sugar-based crosslinker forms a stable atelocollagen hydrogel that is a favorable microenvironment for 3D cell culture. J Biomed Mater Res Part A 2014:102A:4309-4316.

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Effect of Hemolysis, High Bilirubin, Lipemia, Paraproteins, and System Factors on Therapeutic Drug Monitoring

Datta¹

Handbook of Drug Monitoring Methods

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Evaluation of HemogloBind™ for Removal of o-Raffinose Cross-Linked Hemoglobin (Hemolink™) from Serum

Cited by 8 publications

References 7 publications

Using the hemoglobin-binding Staphylococcus aureus protein IsdH to enable plasma analysis of hemolyzed blood samples

Using the hemoglobin-binding Staphylococcus aureus protein IsdH to enable plasma analysis of hemolyzed blood samples

Sugar-based crosslinker forms a stable atelocollagen hydrogel that is a favorable microenvironment for 3D cell culture

Effect of Hemolysis, High Bilirubin, Lipemia, Paraproteins, and System Factors on Therapeutic Drug Monitoring

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