Evaluation of hemopoietic responses in<i>Labeo rohita</i>Hamilton following acute copper toxicity

Som, Mousumi; Kundu, Niloy; Bhattacharyya, Somnath; Homechaudhuri, Sumit

doi:10.1080/02772240801990700

Cited by 9 publications

(9 citation statements)

References 18 publications

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“…As reported by Svobodova et al (1994), excessive Cu levels can cause leukopenia. This is corroborated by Som, Kundu, Bhattacharyya, and Homechaudhuri (2009), who studied lethal concentrations of Cu (100% 72h LC50 -50 mg•dm -3 Cu) in roho labeo Labeo rohita, and observed an initial drop in leukocyte count, followed by an increase. In an experiment by Ajani and Akpoilih (2010), a dietary intake of 1000 mg Cu kg -1 in common carp Cyprinus carpio produced an increase in leukocyte count from 1.38 to 1.63•10 3 mm -3 and a decrease in lymphocyte count (from 69% to 64%).…”

Section: Discussionsupporting

confidence: 57%

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Ewa¹,

Dawid²,

Ewa³

et al. 2019

Turk. J. Fish. Aquat. Sci.

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The purpose of the present paper was to investigate the process of copper elimination and iron concentration variations in the body of Prussian carp after shortterm exposure (2.5 or 5 hours) to a sublethal concentration of this metal in water, and to identify the hematological changes occurring in peripheral blood during exposure and depuration. Prussian carps (Carassius gibelio Bloch, 1782) were exposed to 2 mg Cu L-1 for 2.5 or 5 hours. During the 336 hours of depuration period, a concentration of red blood cells RBC, white blood cells WBC and hematocrit, as well as Cu and Fe concentration in gills, liver, muscle and kidney were determined. Significant decrease in WBC and RBC after the exposure cessation was observed and the increase in hematocrit value. Cu concentration in studied tissues of Prussian carp was the highest after the exposure cessation, and then was decreasing during the recovery period, reaching control values, except the kidney where the Cu level remained significantly increased till the end of the observation. Even a relatively short exposure to Cu is reflected in a change in normal morphological parameters and microelement metabolism in the tissue, and its effects can be observed for as long as 2 weeks following the end of exposure. Potentially, this means that any shortening of acute exposure may contribute to quicker restoration of homeostasis during depuration with regard to the blood parameters and microelement levels studied.

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Section: Discussionsupporting

confidence: 57%

Untitled

Ewa¹,

Dawid²,

Ewa³

et al. 2019

Turk. J. Fish. Aquat. Sci.

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“…The only results were presented by Som et al (2009) who observed an increase in Labeo rohita blast cell apoptosis during both lethal and sublethal Cu exposures, while their proliferation rate increased only under sublethal conditions. These results show concentration-related effect of copper on hematopoietic potential of fish.…”

Section: Discussionmentioning

confidence: 92%

“…Also, Saxena et al (1992) mentioned damage of Heteropneustis fossilis hematopoietic tissue caused by this metal. Considerable hematopoietic disturbances were also observed in Labeo rohita subjected to Cu treatment: 50 % 72hLC 50 —25 mg/dm 3 Cu or 100 % 72hLC 50 —50 mg/dm 3 Cu (Som et al 2009). Sublethal exposure resulted in an increase of abundance of both erythrocyte and leukocyte precursors in head kidney hematopoietic tissue, while under lethal conditions, their number initially decreased, and then increased until the end of the experiment.…”

Section: Discussionmentioning

confidence: 99%

“…Cadmium and copper probably affect not only circulating blood cells, but also newly developing ones in hematopoietic tissue. Very scarce data concerning hematopoietic effects of heavy metals in fish (Garofano and Hirshfield 1982; Ghosh et al 2007; Som et al 2009) and mammals (Lutton et al 1984; Mitsumori et al 1998; Van Den Heuvel et al 1999; 2001; Celik et al 2005, 2009) indicate that they are cytotoxic to precursor cells, and various cell lineages show different sensitivity to metal toxicity.…”

Section: Introductionmentioning

confidence: 99%

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Cadmium and copper reduce hematopoietic potential in common carp (Cyprinus carpio L.) head kidney

Kondera

Witeska

2012

Fish Physiol Biochem

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The effects of cadmium and copper on activity of common carp head kidney hematopoietic tissue were evaluated. The fish were subjected to short-term (3 h, Cd-s and Cu-s) or long-term (4 weeks, Cd-l and Cu-l) exposures to 100 % 96hLC50 or 10 % 96hLC50, respectively. Head kidneys were isolated weekly from 5 fish of each group for 4 weeks (post-short-term exposure and during long-term exposure). Percentage of early blast cells among the hematopoietic precursors was calculated. Proliferative and apoptotic activity were evaluated using immunocytochemical staining for proliferating cell nuclear antigen (PCNA) and caspase 3, respectively. Hematopoietic activity was calculated as the ratio of proliferating to apoptotic cells. All metal exposures induced an increase in frequency of early blast cells. The frequency of proliferating (PCNA-positive) cells also significantly increased. A considerable and significant increase in the frequency of apoptotic cells was the most pronounced effect of metal exposures. Both short-term and long-term treatments caused similar effects, but in case of Cd exposures, the reaction was more pronounced. All metal exposures reduced hematopoietic potential of fish measured as the ratio of proliferating to apoptotic precursor cell frequency. However, in all cases, hematopoietic activity was higher than 1 showing that the rate of repair of hematopoietic tissue prevailed over destruction.

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“…Accumulation of cadmium and copper in fish tissues has been extensively studied by many authors (e.g., De Conto Cinier et al 1997 ; Kraemer et al 2005 ; Calta and Canpolat 2006 ; Celechovska et al 2007 ; Karaytug et al 2007 ; Singh et al 2008 ; Vinodhini and Narayanan 2008 ; Rauf et al 2009 ), but affinity of metals to head kidney (pronephros ) and their toxicity to this organ are almost unknown (Garofano and Hirshfield 1982 ; Ghosh et al 2007 ; Som et al 2009 ; Kondera and Witeska 2012 ).…”

Section: Introductionmentioning

confidence: 99%

High affinity of cadmium and copper to head kidney of common carp (Cyprinus carpio L.)

Kondera

Ługowska

Sarnowski

2013

Fish Physiol Biochem

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The aim of the present study was to evaluate bioaccumulation of metals in various tissues of the freshwater fish Cyprinus carpio L. exposed to cadmium and copper (a xenobiotic and a microelement). The fish were subjected to short-term (3 h, Cd-S and Cu-S) or long-term (4 weeks, Cd-L and Cu-L) exposures to 100 % 96hLC50 or 10 % 96hLC50, respectively. Blood, gill, liver, head and trunk kidney were isolated weekly from 5 fish of each group for 4 weeks (post-short-term exposure and during long-term exposure). Atomic absorption spectrophotometry technique was applied to measure concentrations of metals (Cd and Cu) in fish tissues. Initial concentrations of copper in fish tissues were higher than levels of cadmium. Cadmium and copper levels increased in all tissues of metal-exposed fish. After short-term exposures (at higher concentration) and during long-term exposures (at lower concentration), similar changes in metal concentrations were observed. The values of accumulation factor (ratio of final to initial metal concentration) were higher for cadmium as compared to copper. Comparison of metal levels and accumulation factors in various tissues revealed that cadmium and copper showed very high affinity to head kidney of common carp (higher than to other tissues), but accumulation factors for cadmium in trunk, head kidney and liver were much higher than for copper. The concentrations of copper in organs of Cu-exposed fish increased only slightly and quickly returned to the control level, which shows that fish organism easily buffered metal level. On the other hand, concentrations of cadmium considerably increased and remained elevated for a long time which suggests that activation of mechanisms of sequestration and elimination of cadmium required more time.

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Evaluation of hemopoietic responses inLabeo rohitaHamilton following acute copper toxicity

Cited by 9 publications

References 18 publications

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Cadmium and copper reduce hematopoietic potential in common carp (Cyprinus carpio L.) head kidney

High affinity of cadmium and copper to head kidney of common carp (Cyprinus carpio L.)

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