Evaluation of hepatoprotective and antidiarrhoeal activity of guduchi, Tinospora cordifolia (Willd.) Miers ex Hook. f. & Thoms. in experimental rats

Abstract

The present study investigated the antidiarrhoeal and hepatoprotective potential of 50 % ethanolic extract of stems of Tinospora cordifolia (Willd.) Miers ex Hook. f. & Thoms. in experimental rats. Diarrhoea along with hepatotoxicity was induced by feeding red kidney beans, i.e., Phaseolus vulgaris, mixed with rat feed in the ratio of 30:70 (study diet) along with administering CCl 4 , mixed with olive oil (1:1; v/v) at 4 ml/kg body weight orally twice a week for trial period of 10 days. Group 1 was healthy control; Group 2 was positive control; Group 3 received silymarin (5 mg/kg b.wt orally) and atropine sulfate (0.1 mg/kg, intra muscular OD); Groups 4 and 5 received 50 % ethanolic extract of T. cordifolia at 100 and 200 mg/kg body weight orally, respectively. Results revealed that Group 3 rats restored fecal consistencies from 3 rd day onwards while in Groups 4 and 5, fecal consistency restored from 7 th and 8 th day onwards, respectively. Significant increase in liver enzyme activities due to CCl 4 toxicity was restored well in Group 3, followed by the group receiving T. cordifolia at 200 mg/kg. No significant changes were noticed in blood urea nitrogen, creatinine and total bilirubin level within all groups. Oxidative stress indices like lipid peroxida tion (3.03 ± 0.1 2 nmol MDA/ mg protein) and glutathione (6 .46 ± 0.3 8 mol/mg protein) were significantly improved in Group 3, followed by group receiving T. cordifolia at 200 mg, whereas superoxide dismutase (8.13 ± 0.1 U/mg protein) and catalase (7.94 ± 0.3 U/mg protein) were improved better in Group 3, followed by Group 5 receiving T. cordifolia at 100 mg. Histopathological study of liver revealed the changes like hepatic degeneration, vacuolation, and dissolution of cytoplasm in negative control group. Silymarin treated group revealed normalcy in hepatic cord with mild vacuolar changes. T. cordifolia at 200 mg/kg treatment group revealed only mild fatty changes in liver. This study revealed the dose-dependent hepatoprotectant and antidiarrhoeal effect of T. cordifolia in experimental rats.

Assessment of Anti-Hyperglycemic and Anti-Oxidant Activities of Tinospora Cordifolia & Juglans Regia Composite Extract in STZ Induced Diabetes in Wistar Rats

“…The extract of Tinospora cordifolia produced protective effects in the kidney against lead toxicity (Sharma and Pandey, 2010). The extract of Tinospora cordifolia (100mg/kg) changed in the centrilobular region and vacuolar congestion and graded 1-2 by hepatoprotective system (Gurav et al, 2017). Pancreas section of STZ induced diabetes rats showed damaged pancreatic islets cells and the extract of Tinospora cordifolia comparatively less degeneration of Islets of Langerhans and degranulation (Sreshta et al, 2018) which was also similar to our inding.…”