Evaluation of Hepatoprotective effect of hydroalcoholic extract of Momordica Charantia leaves in carbon tetrachloride induced liver toxicity in Wistar rats

Abstract

Liver carries out a variety of physiological functions and protects against damaging drugs and chemicals. Herbs have been shown to play a major role in the management of various liver disorders. Due to the lack of effective liver protective medication in modern medicine, several herbal options for the treatment of liver diseases in Ayurveda are suggested. In this current study, we evaluated the hepatoprotective action of Momordica charantia leaf extract in comparison to Liv-52, a standard hepatoprotective drug. In Wistar rats, hepatotoxicity was induced by administering carbon tetrachloride (CCl4) 1ml / kg body weight subcutaneously on alternate days for a week in a suspension of liquid paraffin. Rats were grouped into 5 groups with group I as control, group II - CCl4 treatment only, group III receiving a mixture of Liv-52 orally (5 ml / kg) and CCl4, and group IV and group V receiving Momordica charantia leaf extract administered orally to rats at doses of 100 and 200 mg / kg respectively, together with CCl4 for 1 week.. Indices of liver functions (lipid profile) were evaluated in the serum of the rats. Animals were sacrificed after the study period and liver tissue was isolated for histopathological changes. The mean results for groups I to V for SGOT levels in IU/L were: 53.57 ± 1.19, 167.72 ± 5.57, 54.72 ± 0.83, 69.41 ± 2.35 and 60.72 ± 1.5 respectively; for SGPT in IU/L were 37.00 ± 1.77, 118.16 ± 2.91, 61.41 ± 1.25, 47.92 ± 1.71 and 58.59 ± 1.81 respectively; for ALP in IU/L were 165.44 ± 4.84, 281.33 ± 7.11, 206 ± 6.95, 190.62±5.47 and 188.86 ± 2.5 respectively and for total bilirubin levels in mg/dl were 0.71± 0.66, 1.57 ± 0.1, 0.80 ± 0.20.88 ± 0.02 & 0.77 ± 0.03 respectively. The findings from this study showed a decrease in the liver enzymes and therefore suggests protective activity of Momordica charantia leaf extract against CCl4 induced hepatic toxicity.

Medicinal herbs and their metabolites with biological potential to protect and combat liver toxicity and its disorders: A review

“… [ 157 ] Momordica charantia Linn. (Cucurbitac-eae) Hydro-alcoholic extract of Leaves Rats Carbon tetrachloride 100 & 200 mg/kg Total bilirubin↓, ALP↓, SGPT↓, SGOT↓ Antimicrobial [ 158 ] Momordica dioica (Cucurbita-ceae) Ethanolic and aqueous extract of Leaves Rats Carbon tetrachloride 200 mg/kg AST↓, ALT↓, ALP↓, total bilirubin (TB)↓ Used in head trouble, urinary calculi, tridosha, fever, asthma, bronchitis, high cough and piles. Aphrodisiac and anthelmintic.…”

Ethnomedicinal study and in vitro validation of medicinal plants used for treating Jaundice in Zuru emirate of Kebbi State, Nigeria

“…The leaf decoction is used to treat diabetes and malaria in Nigeria (Ediriweera et al, 2017) Pulp extract combined with lupeol Induced hepatoprotective effect against 7, 12dimethylbenzanthracene induced liver alteration in Swiss albino mice (Shah et al, 2010) Studies have reported the lack of toxic effect although minimal toxic effect on seed kernel has been reported (Derese et al, 2017) Momordica charantia L It is used to treat jaundice in India. It is also used to treat hepatitis (Grover and Yadav, 2004) Hydroalcoholic leaf extract induced hepatoprotective effect against CCl4 induced hepatotoxicity in rats (Moharir et al, 2019) The plant is relatively safe as no toxicity is reported in experimental animals when administered at low dose for up to 2 months (Grover and Yadav, 2004) Moringa oleifera Lam.…”

Đánh Giá Độc Tính Cấp Và Tác Dụng Bảo Vệ Gan Của Cao Cồn Vỏ Thân Núc Nác Thu Hái Tại Thái Nguyên

“…Mặc dù y học hiện đại đã đạt được những bước tiến lớn, song phương pháp điều trị cũng như lựa chọn thuốc điều trị các bệnh về gan hiện vẫn còn nhiều hạn chế. Bên cạnh đó, một số thuốc có nguồn gốc hóa dược dùng trong điều trị bệnh gan cũng có khả năng gây tổn thương gan [1], [2]. Vì vậy, các nghiên cứu về dược liệu có tác dụng dự phòng và điều trị bệnh gan là vấn đề quan trọng và cần thiết.…”