The taxonomic position of a Gram-negative, non-motile, oxidase negative and catalase positive strain, A648 T , isolated from a hexachlorocyclohexane (HCH) dump site located in Lucknow, India, was ascertained by using a polyphasic approach. A comparative analysis of a partial sequence of the rpoB gene and the 16S rRNA gene sequence revealed that strain A648T belonged to the , were found to be less than 8 %. The major cellular fatty acids of strain A648T were 18 : 1v9c (19.6 %), summed feature 3 (15.9 %), 16 : 0 (10.6 %) and 12 : 0 (6.4 %). The DNA G+C content was 40.4 mol%. The polar lipid profile of strain A648 T indicated the presence of diphosphatidylglycerol, phosphatidylethanolamine, followed by phosphatidylglycerol and phosphatidylcholine. The predominant polyamine of strain A648 T was 1,3-diaminopropane and moderate amounts of putrescine, spermidine and spermine were also detected. The respiratory quinone consisted of ubiquinone with nine isoprene units (Q-9). On the basis of DNA-DNA hybridization, phenotypic characteristics and chemotaxonomic and phylogenetic comparisons with other members of the genus Acinetobacter, strain A648 T is found to be a novel species of the genus Acinetobacter, for which the name Acinetobacter indicus sp. nov. is proposed. The type strain is A648 T (5DSM 25388 T 5CCM 7832 T ).