Evaluation of Hiaureus TM  Coagulase  Confirmation Kit in Identification of  Staphylococcus aureus

Subramanian, Arunagiri; Chitalia, Vidushi; Bangera, Kushminda; Vaidya, Shashikant; Warke, Rajas V.; Chowdhary, Abhay; Deshmukh, Ranjana

doi:10.7860/jcdr/2017/24021.9265

Cited by 5 publications

(5 citation statements)

References 25 publications

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“…They also recommended that tube coagulase test should be performed on clinical microbiology laboratory so that we can correctly differentiate Both sensitivity and specificity of slide coagulase test were lower than that of Pastorex Staph plus agglutination test, thereby making the latter a more reliable test. [26]. In contrast to their finding, our study showed that Mannitol Fermentation test has lower specificity (90.3%) than sensitivity (96.8%).…”

Section: Fig 3 Species Wise Distribution Ofcontrasting

confidence: 99%

Evaluation of Pastorex Staph Plus Rapid Agglutination Test to Confirm Staphylococcus aureus Isolated from Clinical Specimens in a Tertiary Care Teaching Hospital

Das¹,

Gupta²,

Chakraborty³

et al. 2018

JAMMR

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Background: Staphylococcus aureus is one of the most common pathogen isolated. Accurate and rapid identification of S. aureus and MRSA is of great importance for proper care of the patient. Keeping this in mind, we had used Pastorex Staph plus rapid agglutination test to confirm S. aureus. Aim: To evaluate Pastorex Staph plus rapid agglutination test for confirming Staphylococcus aureus isolated from clinical specimens. Study Design: Cross-sectional type. Methodology: This study was conducted in a tertiary care hospital in Eastern India. It included all clinical samples, where Staphylococcus spp. was isolated. The phenotypic confirmation of the Original Research Article

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Section: Fig 3 Species Wise Distribution Ofcontrasting

confidence: 99%

Evaluation of Pastorex Staph Plus Rapid Agglutination Test to Confirm Staphylococcus aureus Isolated from Clinical Specimens in a Tertiary Care Teaching Hospital

Das¹,

Gupta²,

Chakraborty³

et al. 2018

JAMMR

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“…In MSA media, the S. epidermidis grow as a pink-color colony. In contrast, S. aureus grows as a yellow-color colony surrounded by a yellow halo on the pink-colored MSA medium (Subramanian et al, 2017). In addition, a Staphylococcus Agar 110 media was used to select particular species that belonged to S. aureus because of pigment formation during the bacteria growth.…”

Section: Discussionmentioning

confidence: 99%

Presence of Antibiotic-Resistant in Staphylococcal Subclinical Mastitis in Several Regencies of East Java, Indonesia

Widianingrum¹,

Khasanah

Addy

2022

Trop. Anim. Sci. J.

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Staphylococcal mastitis has been reported as a serious dairy disease in various regions around the world. The occurrence of resistant strains in Staphylococcus species to antibiotics has triggered alternative treatment substituting antibiotic usage on the global scene. This study aimed to investigate the presence of antibiotic-resistant genes in Staphylococcal subclinical-mastitis cases present in several regencies of East Java Province, Indonesia. A total of 592 quarter milk samples were collected from 62 farms in the region with high dairy cattle populations in Lumajang,

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“…32 And considering the sensitivity and specificity of Mannitol Salt Agar to be 97.86% and 100% respectively. 33 The estimated sample size for true prevalence was 243 all over Western Chitwan. Samples from separate quarters were collected in the labelled universal sample container.…”

Section: Sampling Methods and Sample Size Determinationmentioning

confidence: 99%

Prevalence and Risk Factors of Staphylococcal Subclinical Mastitis in Dairy Animals of Chitwan, Nepal

Tiwari¹,

Subedi²,

Bhandari³

et al. 2022

J. Pure Appl. Microbiol.

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Subclinical mastitis (SCM), the asymptomatic inflammation of breast tissue, is the most common form of mastitis in livestock. SCM prevalence and risk factors in dairy animals in Western Chitwan, Bagmati Province, Nepal, were the primary objectives of this study. Out of 243 dairy animals, 104 representing 42.8% were positive for SCM in the California mastitis test (CMT) in the study area. At the quarter level, out of 972 active quarters tested for SCM, 188 (19.3%) were positive to CMT test. The prevalence of Staphylococcal SCM was 39.92% (97/243) and 18.21% (177/972) at animal level and quarter level, respectively. Coagulase Negative Staphylococcus (CNS) (46.33%) was the most prevalent Staphylococcus to cause SCM at quarter level. While at animal level, SCM due to occurrence of both S. aureus and CNS (36.08%) in an individual was more common. High susceptibility towards Amikacin, Ceftriaxone and Gentamicin was seen against both isolates. Low resistance against Amikacin and Ceftriaxone was seen against both isolates. Ciprofloxacin (41.2% vs 27.4%) and Gentamicin (37.8% vs 23.2%) were more resistance towards CNS whereas Enrofloxacin (41.1% vs 25.2%) and Tetracycline (36.8% vs 15.1%) were more resistant towards S. aureus. Older aged, multiparous and late lactating animals had the highest prevalence of staphylococcal SCM i.e., 58.1%, 56% and 52.6%, respectively. The study concludes that there is a high prevalence of Staphylococcal SCM in Western Chitwan, Nepal. CNS is the most common mastitis pathogen. Increased antimicrobial resistance to S. aureus and CNS could be the result of the indiscriminate use of antibiotic drugs without an antibiotic susceptibility test (AST). This study emphasizes the importance of ongoing antibiotic surveillance, excellent farm and animal hygiene, and suitable housing and feeding management.

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Evaluation of Hiaureus TM Coagulase Confirmation Kit in Identification of Staphylococcus aureus

Cited by 5 publications

References 25 publications

Evaluation of Pastorex Staph Plus Rapid Agglutination Test to Confirm Staphylococcus aureus Isolated from Clinical Specimens in a Tertiary Care Teaching Hospital

Evaluation of Pastorex Staph Plus Rapid Agglutination Test to Confirm Staphylococcus aureus Isolated from Clinical Specimens in a Tertiary Care Teaching Hospital

Presence of Antibiotic-Resistant in Staphylococcal Subclinical Mastitis in Several Regencies of East Java, Indonesia

Prevalence and Risk Factors of Staphylococcal Subclinical Mastitis in Dairy Animals of Chitwan, Nepal

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