Evaluation of human adipose-derived mesenchymal stromal cell Toll-like receptor priming and effects on interaction with prostate cancer cells

Rivera-Cruz, Cosette M.; Figueiredo, Marxa L.

doi:10.1016/j.jcyt.2022.09.009

Cited by 7 publications

(5 citation statements)

References 67 publications

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“…Similar observations have been made for ASCs, which, depending on TLR3 or TLR4 stimuli, could behave as immunomodulatory or inflammatory cells, respectively [104].…”

Section: Role Of Toll-like Receptorssupporting

confidence: 77%

Pathogenic Role of Adipose Tissue-Derived Mesenchymal Stem Cells in Obesity and Obesity-Related Inflammatory Diseases

Pestel

Blangero

Eljaafari

2023

Cells

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Adipose tissue-derived mesenchymal stem cells (ASCs) are adult stem cells, endowed with self-renewal, multipotent capacities, and immunomodulatory properties, as mesenchymal stem cells (MSCs) from other origins. However, in a pathological context, ASCs like MSCs can exhibit pro-inflammatory properties and attract inflammatory immune cells at their neighborhood. Subsequently, this creates an inflammatory microenvironment leading to ASCs’ or MSCs’ dysfunctions. One such example is given by obesity where adipogenesis is impaired and insulin resistance is initiated. These opposite properties have led to the classification of MSCs into two categories defined as pro-inflammatory ASC1 or anti-inflammatory ASC2, in which plasticity depends on the micro-environmental stimuli. The aim of this review is to (i) highlight the pathogenic role of ASCs during obesity and obesity-related inflammatory diseases, such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory bowel disease, and cancer; and (ii) describe some of the mechanisms leading to ASCs dysfunctions. Thus, the role of soluble factors, adhesion molecules; TLRs, Th17, and Th22 cells; γδ T cells; and immune checkpoint overexpression will be addressed.

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“…Similar observations have been made for ASCs, which, depending on TLR3 or TLR4 stimuli, could behave as immunomodulatory or inflammatory cells, respectively [104].…”

Section: Role Of Toll-like Receptorssupporting

confidence: 77%

Pathogenic Role of Adipose Tissue-Derived Mesenchymal Stem Cells in Obesity and Obesity-Related Inflammatory Diseases

Pestel

Blangero

Eljaafari

2023

Cells

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“…The cohabitation of ASCs with pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α, IFN-ɤ, IL-6, IL-1β, and/or IL-17 can potentiate their effectiveness for inflammatory diseases [ 53 , 100 , 101 ]. Numerous investigators (Table 1 ) assessed the immunogenicity and/or immunosuppressive properties of hASCs after treatment with pro-inflammatory cytokine(s) [ 6 , 13 , 14 , 22 , 27 , 61 , 67 , 85 , 93 , 102 – 121 ] or TLR agonist (s) [ 122 – 129 ].…”

Section: Asc Priming/licensing/preconditioningmentioning

confidence: 99%

“…Numerous investigators (Table 1 ) assessed the immunogenicity and/or immunosuppressive properties of hASCs after treatment with pro-inflammatory cytokine(s) [ 6 , 13 , 14 , 22 , 27 , 61 , 67 , 85 , 93 , 102 – 121 ] or TLR agonist (s) [ 122 – 129 ].…”

Section: Asc Priming/licensing/preconditioningmentioning

confidence: 99%

Factors Defining Human Adipose Stem/Stromal Cell Immunomodulation in Vitro

Mahmoud,

Abdel-Rasheed,

Galal

et al. 2023

Stem Cell Rev and Rep

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Human adipose tissue-derived stem/stromal cells (hASCs) are adult multipotent mesenchymal stem/stromal cells with immunomodulatory capacities. Here, we present up-to-date knowledge on the impact of different experimental and donor-related factors on hASC immunoregulatory functions in vitro. The experimental determinants include the immunological status of hASCs relative to target immune cells, contact vs. contactless interaction, and oxygen tension. Factors such as the ratio of hASCs to immune cells, the cellular context, the immune cell activation status, and coculture duration are also discussed. Conditioning of hASCs with different approaches before interaction with immune cells, hASC culture in xenogenic or xenofree culture medium, hASC culture in two-dimension vs. three-dimension with biomaterials, and the hASC passage number are among the experimental parameters that greatly may impact the hASC immunosuppressive potential in vitro, thus, they are also considered. Moreover, the influence of donor-related characteristics such as age, sex, and health status on hASC immunomodulation in vitro is reviewed. By analysis of the literature studies, most of the indicated determinants have been investigated in broad non-standardized ranges, so the results are not univocal. Clear conclusions cannot be drawn for the fine-tuned scenarios of many important factors to set a standard hASC immunopotency assay. Such variability needs to be carefully considered in further standardized research. Importantly, field experts’ opinions may help to make it clearer. Graphical Abstract Parameters that promote ASC immunosuppression on immune cells. Activation of immune cells induces their proliferation and differentiation and presence of ASCs modulates/suppresses such consequences. Augmented immunosuppressive effects of ASCs can be introduced in direct contact with the immune cells and via complementing the repeatedly reported experimental settings (texts in grey shapes). Abbreviations: ASCs: adipose tissue-derived stem/stromal cells, IFN-ɤ: Interferon gamma, MLR: Mixed lymphocyte reaction, TNF: Tumor necrosis factor.

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“…TLR3/4 priming of MSCs may reprogram them toward an anti-tumour phenotype and modulate the immune-mediated killing of tumour cells by up-regulating pro-inflammatory factors. It is linked to the activation of immune cells by MSCs in vitro and the retardation of tumour cells [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Immunogenic cell death mediated TLR3/4-activated MSCs in U87 GBM cell line

Emami Meybodi,

Moradi Moraddahande,

Dehghani Firoozabadi

2024

Heliyon

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Evaluation of human adipose-derived mesenchymal stromal cell Toll-like receptor priming and effects on interaction with prostate cancer cells

Cited by 7 publications

References 67 publications

Pathogenic Role of Adipose Tissue-Derived Mesenchymal Stem Cells in Obesity and Obesity-Related Inflammatory Diseases

Pathogenic Role of Adipose Tissue-Derived Mesenchymal Stem Cells in Obesity and Obesity-Related Inflammatory Diseases

Factors Defining Human Adipose Stem/Stromal Cell Immunomodulation in Vitro

Immunogenic cell death mediated TLR3/4-activated MSCs in U87 GBM cell line

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