Evaluation of human and microbial DNA content in subgingival plaque samples collected by paper points or curette

Chemomechanical preparation influences the microbial community and the levels of LPS, LTA and cytokines in combined endodontic‐periodontal lesions: A clinical study

“…In the PP, there was a higher prevalence of Desulfobulbus, Streptococcus, Parvimonas, Enterococcus, Fusobacterium, Actinomyces, Tannerella, Filifactor, and Peptostreptococcus. Moreover, species such as Parvimonas micra, Enterococcus faecalis, Filifactor alocis, Streptococcus constellatus, Streptococcus sanguinis, and Peptostreptococcaceae [XI][G‐9] [Eubacterium] brachy predominated, agreeing with previous studies 22,36,42,43 …”

Increased Levels of Dissolved Titanium Are Associated With Peri‐Implantitis – A Cross‐Sectional Study

“…Curets were used for sampling because they are considered superior to paper strips as a means of collecting bacterial DNA for quantitative analysis. 24 This approach was found to be robust for the analyses performed in the study because: 1) a significantly higher quantity of submucosal plaque was collected from sites with peri-implantitis than expected due to deeper PDs and disease activity at those sites; and 2) titanium levels in the healthy group were very close to zero, confirming the use of the curets did not lead to interference with the implant surface. Previous work has recorded the immunogenic effect on the participation of the products of titanium corrosion in amplifying host inflammatory response and enhancing bone loss in peri-implantitis lesions by means of an increased release of proinflammatory cytokines, 15 TNF-a, 11 IL-1b, 14 and RANKL 17 by host cells in the presence of titanium products.…”

“…The current study used submucosal plaque as a non‐invasive and easily accessible media for collection and subsequent quantification of relative amounts of dissolved titanium. Curets were used for sampling because they are considered superior to paper strips as a means of collecting bacterial DNA for quantitative analysis 24 . This approach was found to be robust for the analyses performed in the study because: 1) a significantly higher quantity of submucosal plaque was collected from sites with peri‐implantitis than expected due to deeper PDs and disease activity at those sites; and 2) titanium levels in the healthy group were very close to zero, confirming the use of the curets did not lead to interference with the implant surface.…”

Investigation of a Novel Predictive Biomarker Profile for the Outcome of Periodontal Treatment

“…However, F. nucleatum was included as a positive internal control because it would be expected to be present in all samples because it acts as a “bridging” species in the oral biofilm 31,32 . Moreover, plaque samples were collected using curets, which others have shown to yield higher levels of bacterial DNA than paper points 32,33 …”