Evaluation of CD109, PCP4 and SEMA3D genes for their association with Ovine Johne’s disease in Turkish sheep

Yaman, Yalçın; Aymaz, Ramazan; Keleş, Murat; Bay, Veysel; Hatipoğlu, Ecem; Kaptan, Cuneyt; Başkurt, Atilla; Yılmaz, Onur; Heaton, Michael P.

doi:10.1080/10495398.2021.1887880

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…Another gene significantly associated with OsHV-1 mortality encodes CD109 of the thioester-containing proteins superfamily, which plays important roles in activating T-cells and endothelial cells (93)(94)(95), affecting cell proliferation, cell death and other processes by negatively regulating transforming growth factor beta (TGF-beta) signaling pathway (96). The gene encoding CD109 was associated mortality from Ovine Johne's disease in Turkish sheep (97). Therefore, variations near CD109 may affect OsHV-1 resistance through its regulation of cell proliferation via TGF-beta signaling pathway, affecting OsHV-1 pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas

Yao

Guan

et al. 2022

Front. Immunol.

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The Ostreid herpesvirus 1 (OsHV-1) is a lethal pathogen of the Pacific oyster (Crassostrea gigas), an important aquaculture species. To understand the genetic architecture of the defense against the pathogen, we studied genomic variations associated with herpesvirus-caused mortalities by pooled whole-genome resequencing of before and after-mortality larval samples as well as dead and surviving adults from a viral challenge. Analysis of the resequencing data identified 5,271 SNPs and 1,883 genomic regions covering 3,111 genes in larvae, and 18,692 SNPs and 28,314 regions covering 4,863 genes in adults that were significantly associated with herpesvirus-caused mortalities. Only 1,653 of the implicated genes were shared by larvae and adults, suggesting that the antiviral response or resistance in larvae and adults involves different sets of genes or differentiated members of expanded gene families. Combined analyses with previous transcriptomic data from challenge experiments revealed that transcription of many mortality-associated genes was also significantly upregulated by herpesvirus infection confirming their importance in antiviral response. Key immune response genes especially those encoding antiviral receptors such as TLRs and RLRs displayed strong association between variation in regulatory region and herpesvirus-caused mortality, suggesting they may confer resistance through transcriptional modulation. These results point to previously undescribed genetic mechanisms for disease resistance at different developmental stages and provide candidate polymorphisms and genes that are valuable for understanding antiviral immune responses and breeding for herpesvirus resistance.

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Section: Discussionmentioning

confidence: 99%

Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas

Yao

Guan

et al. 2022

Front. Immunol.

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“…It is clear that in molecular genetic studies, the cell types used for DNA extraction directly affects FAW, which has caused ethical concerns. In scientific reports, permission is required from local or global ethical councils/committees before collection of samples for molecular genetic studies conducted on different farm animals such as cattle, goats, sheep, and donkeys (Guang-Xin et al ., 2019; Öner et al ., 2019; Yatkın et al ., 2020; Yaman et al ., 2021).…”

Section: Direct Contributions Of Molecular Genetics To Fawmentioning

confidence: 99%

Direct and indirect contributions of molecular genetics to farm animal welfare: a review

Demir

Bilginer

Balcıoğlu

et al. 2021

Anim. Health. Res. Rev.

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Since domestication, farm animals have played a key role to increase the prosperity of humankind, while animal welfare (AW) is debated even today. This paper aims to comprehensively review the contributions of developing molecular genetics to farm animal welfare (FAW) and to raise awareness among both scientists and farmers about AW. Welfare is a complex trait affected by genetic structure and environmental factors. Therefore, the best welfare status can be achieved not only to enhance environmental factors such as management and feeding practices, but also the genetic structure of animals must be improved. In this regard, advances in molecular genetics have made great contributions to improve the genetic structure of farm animals, which has increased AW. Today, by sequencing and/or molecular markers, genetic diseases may be detected and eliminated in local herds. Additionally, genes related to diseases or adaptations are investigated by molecular techniques, and the frequencies of desired genotypes are increased in farm animals to keep welfare at an optimized level. Furthermore, stress on animals can be reduced with DNA extraction from stool and feather samples which reduces physical contact between animals and veterinarians. Together with molecular genetics, advances in genome editing tools and biotechnology are promising to improve FAW in the future.

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Retrospective Single Nucleotide Polymorphism Analysis of Host Resistance and Susceptibility to Ovine Johne’s Disease Using Restored FFPE DNA

Kravitz,

Liao,

Morota

et al. 2024

IJMS

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Johne’s disease (JD), also known as paratuberculosis, is a chronic, untreatable gastroenteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP) infection. Evidence for host genetic resistance to disease progression exists, although it is limited due to the extended incubation period (years) and diagnostic challenges. To overcome this, previously restored formalin-fixed paraffin embedded tissue (FFPE) DNA from archived FFPE tissue cassettes was utilized for a novel retrospective case-control genome-wide association study (GWAS) on ovine JD. Samples from known MAP-infected flocks with ante- and postmortem diagnostic data were used. Cases (N = 9) had evidence of tissue infection, compared to controls (N = 25) without evidence of tissue infection despite positive antemortem diagnostics. A genome-wide efficient mixed model analysis (GEMMA) to conduct a GWAS using restored FFPE DNA SNP results from the Illumina Ovine SNP50 Bead Chip, identified 10 SNPs reaching genome-wide significance of p < 1 × 10−6 on chromosomes 1, 3, 4, 24, and 26. Pathway analysis using PANTHER and the Kyoto Encyclopedia of Genes and Genomes (KEGG) was completed on 45 genes found within 1 Mb of significant SNPs. Our work provides a framework for the novel use of archived FFPE tissues for animal genetic studies in complex diseases and further evidence for a genetic association in JD.

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Evaluation of CD109, PCP4 and SEMA3D genes for their association with Ovine Johne’s disease in Turkish sheep

Cited by 3 publications

References 24 publications

Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas

Pooled resequencing of larvae and adults reveals genomic variations associated with Ostreid herpesvirus 1 resistance in the Pacific oyster Crassostrea gigas

Direct and indirect contributions of molecular genetics to farm animal welfare: a review

Retrospective Single Nucleotide Polymorphism Analysis of Host Resistance and Susceptibility to Ovine Johne’s Disease Using Restored FFPE DNA

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