Evaluation of<i>In Vivo</i>‐Derived Bovine Embryos

Jahnke, Marianna M.; West, James K.; Youngs, Curtis R.

doi:10.1002/9781118833971.ch79

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…Embryos produced in this manner are called in vivo derived (IVD) embryos. Harvested IVD embryos are evaluated (Jahnke et al 2015), and those deemed to be viable (an average of nearly seven IVD embryos per donor) are transferred individually into the uterus of a synchronized recipient female. The degree of synchrony of estrus between donor and recipient females plays an important role in the establishment of an ET pregnancy, and the aforementioned methods for synchronization of estrus and ovulation are routinely used in the ET industry.…”

Section: Embryo Transfer Embryo Transfer Is a Reproductivementioning

confidence: 99%

Modern Reproductive Technologies to Improve Cattle Production

Youngs

2016

Ceiba

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Abstract. Cattle contribute significantly to the global supply of animal-derived proteins which are an important part of a well-balanced human diet. With the human population increasing by 1 billion people every 13 years, there will be an estimated 9.6 billion people on planet Earth by the year 2050. To attain global food security the amount of available food will need to double between now and then. Enhancing reproductive efficiency is a prerequisite for boosting production of meat and milk from cattle, and, fortunately, an arsenal of modern reproductive technologies is available to assist with that effort. The objective of this manuscript is to provide an overview of reproductive biotechnologies that can bolster the efficient production of meat and milk from cattle. Protocols for synchronization of estrus and synchronization of ovulation facilitate more efficient artificial insemination using conventional or sexsorted semen. In vivo and in vitro production of preimplantation embryos from genetically superior females enable creation of multiple offspring with high production potential. Biochemical and ultrasonographic methods for pregnancy testing identify non-pregnant females that can be re-mated or sold to prevent wastage of valuable feed resources. Somatic cell nuclear transfer is used to create copies of highly productive animals, and genome editing of zygotes provides a novel opportunity to selectively enhance the genetic makeup of cattle for the benefit of animal and human health. Wise use of these reproductive technologies will increase food production from cattle and will help alleviate world hunger.Key words: Embryo transfer, in vitro fertilization, pregnancy testing, sperm sexing, synchronization of estrus. Tecnologías Reproductivas Modernas para Mejorar la Producción PecuariaResumen. El ganado contribuye significativamente al suministro global de proteínas de origen animal que son una parte importante de una dieta humana bien balanceada. Con el incremento de la población a un ritmo de mil millones de personas cada 13 años, habrá aproximadamente 9.6 mil millones de personas en el año 2050. Para alcanzar una seguridad alimentaria global, la cantidad de comida disponible deberá ser el doble entre la actual y la de esta fecha futura. Mejorar la eficiencia reproductiva es un prerrequisito para aumentar la producción de carne y leche, y afortunadamente, un arsenal de tecnologías reproductivas modernas está disponibles para asistir con este esfuerzo. El objetivo de este artículo es proveer un resumen de las biotecnologías reproductivas que pueden mejorar la eficiencia de producción de carne y leche. Con los protocolos para la sincronización del celo y de la ovulación se logra una inseminación artificial más eficiente usando semen convencional o sexado. La producción in vitro e in vivo de embriones a partir de hembras genéticamente superiores ayuda a la creación de crías múltiples con un alto potencial de producción. Los métodos bioquímicos y ultrasonográficos identifican de hembras vacías que pueden volver ...

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Section: Embryo Transfer Embryo Transfer Is a Reproductivementioning

confidence: 99%

Modern Reproductive Technologies to Improve Cattle Production

Youngs

2016

Ceiba

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Effect of embryo quality and developmental stages on pregnancy rate during fresh embryo transfer in beef heifers

Erdem

Karaşahi̇n

Alkan

et al. 2020

Trop Anim Health Prod

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Evaluation of pig oocyte in vitro maturation and fertilization using three gonadotropin-based hormonal compounds

Santiago-Rodriguez

Álvarez-Guerrero

García-González

et al. 2021

Asian Pacific Journal of Reproduction

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Objective: To evaluate the effect of human chorionic gonadotropins (hCG) and equine chorionic gonadotropins (eCG) on in vitro gilt oocyte maturation and embryonic development, using frozen semen for fertilization. Methods: Two independent experiments (6 replicates each) were carried out to evaluate gilt oocyte maturation, and fertilization and embryonic development by using ovaries from a local abattoir. Totally, 712 oocytes were randomly distributed in four-well dishes to receive Novormon (eCG 5.0 IU), PG600 (eCG 5.0 IU and hCG 2.5 IU), Chorulon (hCG 5.0 IU), or no hormones. Oocytes were incubated with 5% CO2, 95% air and saturation humidity at 39 °C for 44 h. Maturation of the oocytes to metaphase II was assessed by using the aceto-orcein technique. In addition, 741 oocytes were used and randomly distributed in four-well dishes, and then oocyte maturation was carried out as mentioned, but matured oocytes were washed and placed in fertilization medium with frozen-thawed sperm. Gametes were co-incubated for 7 h, and then washed and placed in development medium, and incubated for further 7 days, at which time embryonic development was evaluated. Fertilization and embryo development media were not supplemented with the studied hormones. Results: Novormon (eCG) and PG600 (eCG+hCG) treatments significantly improved the percentages of metaphase II oocytes compared to the control group (P<0.05). Furthermore, a significant increase was also observed in the young blastocyst stage between the control group and the PG600 treatment group (P<0.05). Conclusions: Hormonal products Novormon (eCG) and PG600 (eCG+hCG) can obtain the highest percentages of in vitro maturation in gilt oocytes; however, this effect is not transferred to fertilization rates.

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Evaluation ofIn Vivo‐Derived Bovine Embryos

Cited by 3 publications

References 17 publications

Modern Reproductive Technologies to Improve Cattle Production

Modern Reproductive Technologies to Improve Cattle Production

Effect of embryo quality and developmental stages on pregnancy rate during fresh embryo transfer in beef heifers

Evaluation of pig oocyte in vitro maturation and fertilization using three gonadotropin-based hormonal compounds

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