Evaluation of<i>In Vivo</i>Wound Healing Activity of<i>Bacopa monniera</i>on Different Wound Model in Rats

Murthy, S Mahadeva; Gautam, Meenu; Goel, Shalini; Purohit, Vinay; Sharma, Hemlata; Goel, Raj Kumar

doi:10.1155/2013/972028

Cited by 154 publications

(123 citation statements)

References 32 publications

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“…Further, acute inflammatory marker myeloperoxidase was estimated by homogenizing granulation tissue in 0.5% hexadecyl trimethyl ammonium bromide (HTAB, Sigma-Aldrich, Co., St. Louis, MO, USA) with 50 mM potassium phosphate buffer (pH 6). The homogenate was freeze-thawed three times; sonicated for 10 s followed by cool centrifugation at 4°C for 45 min at 14,000 Â g. The supernatant was then used for evaluation of Myeloperoxidase (MPO) as per standard procedure (Murthy et al, 2013).…”

Section: Biochemical Estimationsmentioning

confidence: 99%

Systematic investigation of ethanolic extract from Leea macrophylla : Implications in wound healing

Joshi

Pandey

et al. 2016

Journal of Ethnopharmacology

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Section: Biochemical Estimationsmentioning

confidence: 99%

Systematic investigation of ethanolic extract from Leea macrophylla : Implications in wound healing

Joshi

Pandey

et al. 2016

Journal of Ethnopharmacology

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“…Connective tissue parameter such as hydroxyproline content in granulation tissue was also determined by standard methods. 48 Part of the granulation tissue was collected in phosphate-buffered saline for the estimation of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT).…”

Section: Dead Space Wound Modelmentioning

confidence: 99%

Evaluation of Antioxidant, Toxicological and wound healing Properties of Hibiscus rosa-sinensis L. (Malvaceae) ethanolic leaves extract on different Experimental animal models

Mondal¹,

Ghosh²,

Sagar³

et al. 2016

IJPER

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Objective: The present research article was conducted to study the toxicity profile, antioxidant potential and wound healing activity of ethanolic leaves extract of Hibiscus rosa-sinensis L. (ELEHR). Materials and Methods: In-vitro antioxidant properties were assessed using DPPH radical scavenging activity, Nitric oxide scavenging activity, and superoxide radical scavenging activity. Acute toxicity was performed to study the general behavioural pattern of mice and sub-acute toxicity studies were performed to study the toxic effects of the test extract on different biochemical and haematological parameters, body and organ weight and histopathology of liver, pancreas and kidney. Wound healing properties was evaluated using excision, incision, dead space and burn wound model. Results: Strong antioxidant activity was recorded in a concentration dependant manner for ELEHR in all three models. The toxicological evaluation of ELEHR revealed that it has a reasonable safety profile as there was no severe alteration. In the evaluation of wound healing properties, ELEHR treated group showed an enhanced wound contraction rate and epithelisation period in both excision and burn wound models, whereas in incision wound model wound breaking strength was significantly increased in extract treated group compared to control. Histology of the skin of healed excised wound also showed restoration to normal architecture in extract treated group. Wound healing activity was further substantiated by enhanced antioxidants and connective tissue markers which were estimated in dead space wound model. Conclusion: Thus ELEHR proved to be effective in wound healing which may be because of strong antioxidant properties and also possesses reasonable safety profile.

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“…Hexosamines are components of glycosaminoglycans which constitute the basis of the newly formed extracellular matrix. Increased hexosamines levels in MZL‐ointment treated group compared to controls displayed stabilization of collagen fibres by increasing electrostatic and ionic bonds with it (Murthy et al, ; Shetty, Udupa, & Udupa, ).…”

Section: Discussionmentioning

confidence: 99%

In vivo wound healing potential and HPLC-PDA-ESI-MS profiling ofZizyphus lotusL. (Desf.) leaves methanol extract

et al. 2018

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This study was performed to evaluate the wound healing potential and antioxidant effect of methanol extract of Zizyphus lotus leaves (MZL), and to identify its phenolic profile by HPLC‐PDA‐ESI‐MS. Wound healing activity was assessed in vivo using excision model. Biochemical and histopathological analyses were conducted. In vitro antioxidant potential of MZL was tested using 2,2‐diphenyl‐1‐picrylhydrazyl and β‐carotene bleaching methods. HPLC‐PDA‐ESI‐MS was used for the phenolic profiling. Animals treated with MZL ointment showed a significant reduction in epithelialization time and high contraction rate compared to controls. Proteins, hydroxyproline, and hexosamines contents of ointment‐treated animals were higher than controls. Marked cross‐linking of collagen fibers and lack of inflammatory cells were observed in the granulation tissue of the ointment‐treated animals. Chemical profiling of MZL revealed that quercetin‐3‐O‐rutinoside and catechin are the major constituents. Phenolic compounds of the methanol extract of Zizyphus lotus leaves may be engaged in the acceleration of the healing process activity. Practical application In Mediterranean region, like Algeria, Zizyphus lotus is used in nutrition, health, and cosmetics. Several parts of this plant are employed to treat urinary troubles, diabetes, skin infections, fever, diarrhea, insomnia, bronchitis, and hypoglycemia. There is no scientific paper investigating the phenolic composition of Z. lotus leaves using a mass spectrometry coupled high‐performance liquid chromatography, as well as examining their wound healing potential. Results from this study revealed the presence of many flavonoids exhibiting important antioxidant and wound healing potentials through specific mechanisms. Z. lotus leaves are a source of pharmacologically active compounds with benefits for human nutrition, health‐promoting, and disease preventing.

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Evaluation ofIn VivoWound Healing Activity ofBacopa monnieraon Different Wound Model in Rats

Cited by 154 publications

References 32 publications

Systematic investigation of ethanolic extract from Leea macrophylla : Implications in wound healing

Systematic investigation of ethanolic extract from Leea macrophylla : Implications in wound healing

Evaluation of Antioxidant, Toxicological and wound healing Properties of Hibiscus rosa-sinensis L. (Malvaceae) ethanolic leaves extract on different Experimental animal models

In vivo wound healing potential and HPLC-PDA-ESI-MS profiling ofZizyphus lotusL. (Desf.) leaves methanol extract

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